The degree of cumulation of the “Bendamin” drug in the body of white rats
Repeated introduction into the body of an animal of a medicinal substance can lead to its accumulation in the tissues of the body. Therefore, knowledge of the cumulative properties of substances or conditions that may lead to cumulations, are especially important for understanding the pathogenesis of intoxication, because this phenomenon often lies at the basis of acute and chronic poisoning. The purpose of the work was to study the degree of cumulation of the “Bendamin” preparation in the body of white rats. The properties of the cumulative drug were studied in 12 white rats weighing 150–160 g. Rats were divided into 2 groups: control and experimental. To determine the cumulative properties of Bendamin, it was administered to laboratory animals starting at a dose of 0.1 DL50, with a sequential increase in the dose of 1.5 times every 4 days. It was found that the cumulative coefficient of Bendamin was 8.31 units, indicating that the drug does not exhibit cumulative effects. In determining the mass coefficients of the internal organs in the animals of the experimental group, the tendency to increase the weight and liver mass coefficients was determined, respectively, by 5.2 and 5.1%. The weight ratio of the heart of the animals of the experimental group increased by 5.7% relative to the control group. After studying the morphological parameters of blood of rats at 24 days of the experiment for studying the cumulative properties of the “Bendamin” drug, an increase in the number of erythrocytes to 6.13 ± 0.27 T/L, white blood cells to 8.42 ± 0.54 G/L and hemoglobin up to 132.4 ± 2.44 g/l. In the analysis of leukocyte profile in rats, there was a decrease in the number of eosinophils and monocytes, respectively, at 1.1 and 1.3% relative to the control group. The number of lymphocytes in the blood of the experimental group of rats increased by 1.5%, while neutrophils by 0.9% compared to the control. When studying the level of total protein, its small increase was determined by 5.2%. The activity of aminotransferases in the blood serum of experimental rats on the 24th day of the experiment for the study of cumulative properties of the drug also increased by 13 and 8% relative to the control group. Also, creatinine and total bilirubin levels in their rat blood were decreased by 3.1 and 8.8%, respectively.
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