Protein synthesize function of bulls liver at experimental chronic cadmium toxicity


Keywords: toxicology, cadmium, bulls, blood, proteins, albumins, globulins

Abstract

The article presents the results of studies on the influence of cadmium load on the body of young cattle. The adverse effect of Cadmium on the liver causes a wide range of pathological changes at various levels of its organization. The purpose of the work was to investigate protein synthesize function on the liver of bulls for experimental chronic cadmium toxicity. The research was carried out on the basis of a farm at the village Ivanivtsi, Zhydachiv district of Lviv region, on 10 bulls of six months old, Ukrainian black-and-white milk breed, which were formed in 2 groups of 5 animals in each: control and experimental. Bulls of the control group were in the usual diet. Bulls of the experimental group were fed with forage with cadmium chloride at a dose of 0.04 mg/kg body weight of the animal. The research lasted for 30 days. Protein synthesize function of the liver was determined by the level of serum total protein and protein fractions. It was established that when feeding cows with feed of cadmium chloride in a dose of 0.04 mg/kg of body weight of an animal for 30 days in animals suppressed protein synthesize function of the liver, which manifests itself by lowering the total protein of blood. It reached the lowest level at the 20th day of the experiment, where, accordingly, it fluctuated within the limits of 61.9 ± 1.14 g/l. Reducing the level of total protein in the blood of the bulls of the experimental group under cadmium loading was due to lowering the level of albumin. At day 20 of the experiment, the level of albumin in the blood of animals in the experimental group decreased by 16.5%. On the background of general hypoproteinemia there is a significant imbalance between albumins and globulins in serum of diseased animals. It was established that the level of globulins increased in the blood of Bulls, which were under cadmium load. Increasing the level of globulins in blood serum of bulls reflects the intensity of inflammatory processes in their body for cadmium loading.

Downloads

Download data is not yet available.

References

Ali, M.M., Murthy, R.C., & Chandra, S.V. (1986). Developmental and longterm neurobehavioral toxicity of low–level in utero Cd exposure in rats. Neurobehavioral Toxicology and Teratology, 8(5), 463–468. https://www.ncbi.nlm.nih.gov/pubmed/3785508.

Cherkashina, D.V., & Petrenko, A.Y. (2006). Hepatopro-tective effect of fetal tissue cytosol and its thermosta-ble fraction in rats with carbon tetrachloride-induced hepatitis. B. Exp. Biol. Med., 141(4), 544–547. doi: 10.1007/s10517-006-0216-y.

Fregoneze, J.B., Marinho, C.A., Soares, T., Castro, L., Sarmento, C., Cunha, M., Gonzalez, V., Oliveira, P., Nascimento, T., Luz, C.P., Santana, Jr. P., De-Oliveira, I.R., & e-Castro-e-Silva, E. (1997). Lead (Pb2+) and cadmium (Cd2+) inhibit the dipsogenic action of cen-tral beta-adrenergic stimulation by isoproterenol. Bra-zilian Journal of Medical and Biological Research, 30(3), 419–423. doi: 10.1590/S0100-879X1997000300018.

Gutyj, B., Grymak, Y., Drach, M., Bilyk, O., Matsjuk, O., Magrelo, N., Zmiya, M., & Katsaraba, O. (2017). The impact of endogenous intoxication on biochemical indicators of blood of pregnant cows. Regulatory Mechanisms in Biosystems, 8(3), 438–443. doi: 10.15421/021768.

Gutyj, B., Hufriy, D., Binkevych, V., Vischur, V., Binkevych, O., & Kurlyak, I. (2015). The changes of biochemical and morphological indices of rats’ blood under chronic cadmium toxicosis. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 17(3), 120–123. Retrieved from https://nvlvet.com.ua/index.php/journal/article/view/531.

Gutyj, B.V., Gufrij, D., Binkevych, V., Binkevych, O., Kurlyak, I., & Sobolta, A. (2015). Influence of Mevesel & E-selenium on level of intermediate and final products of lipid peroxidation in bulls’ blood after cadmium loading. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 17(1), 190–194. Retrieved from https://nvlvet.com.ua/ index.php/journal/article/view/292.

Gutyj, B.V., Binkevych, V., & Binkevych, O. (2016). Hematological changes of rats after cadmium toxicosis. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 18(1), 165–167. Retrieved from https://nvlvet.com.ua/index.php/ journal/article/view/110.

Gutyj, B.V., Murs'ka, S.D., Gufrij, D.F., Hariv, I.I., Lev-kivs'ka, N.D., Nazaruk, N.V., Gajdjuk, M.B., Pryjma, O.B., Bilyk, O.Ja., & Guta, Z.A. (2016). Influence of cadmium loading on the state of the antioxidant sys-tem in the organism of bulls. Visnyk of Dniprope-trovsk University. Biology, ecology, 24(1), 96–102. doi: 10.15421/011611.

Gutyj, B., Martyshchuk, T., Bushueva, I., Semeniv, B., Parchenko, V., Kaplaushenko, A., Magrelo, N., Hirkovyy, A., Musiy, L., & Murska, S. (2017). Mor-phological and biochemical indicators of blood of rats poisoned by carbon tetrachloride and subject to ac-tion of liposomal preparation. Regulatory Mecha-nisms in Biosystems, 8(2), 304–309. doi: 10.15421/021748.

Gutyj, B., Grymak, Y., Drach, M., Bilyk, O., Matsjuk, O., Magrelo, N., Zmiya, M., & Katsaraba, O. (2017). The impact of endogenous intoxication on biochemical indicators of blood of pregnant cows. Regulatory Mechanisms in Biosystems, 8(3), 438–443. doi: 10.15421/021768.

Hariv, M.I., & Gutyj, B.V. (2016). Vplyv liposomalnoho preparatu Butaintervit na proteinsyntezuvalnu funktsiiu pechinky shchuriv za otruiennia tetrakhlor-metanom [Influence of the liposomal preparation Bu-taintervite on protein synthesis function in the livers of rats under the influence of carbon tetrachloride poi-soning]. Visnyk of Dnipropetrovsk University. Biolo-gy, medicine, 7(2), 123–126. doi: 10.15421/021622 (in Ukrainian).

Honskyy, Ya.I., Yastremskaya, S.O., & Boychuk, B.R. (2001). Vikovi osoblyvostsi porushennya peroksyd-noho okyslennya lipidiv i aktyvnosti enerhozab-ezpechuvalnyh fermentiv pry kadmiyeviy intoksykatsiyi [Age features breach of lipid peroxida-tion and activity of enzymes in utility cadmium intox-ication] Medichna chimiya – Medical Chemistry, 3(1), 16–19 (in Ukrainian).

Hutyi, B.V. (2013). Riven pokaznykiv nefermentnoi systemy antyoksydantnoho zakhystu orhanizmu bychkiv za umov kadmiievoho navantazhennia. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii im. Gzhytskoho, 15, 1(4), 40–45. Rezhym dostupu: http://nbuv.gov.ua/UJRN/nvlnu_2013_15_1(4)__10 (in Ukrainian).

Hutyi, B.V. (2013). Vmist vitaminiv A i E u krovi bychkiv za umov kadmiievoi intoksykatsii. Visnyk Sumskoho natsionalnoho ahrarnoho universytetu. Seriia: Veterynarna medytsyna, 2, 31–33. Rezhym dostupu: http://nbuv.gov.ua/UJRN/Vsna_vet_2013_2_10 (in Ukrainian).

Hutyi, B.V. (2013). Vplyv E-selenu na aktyvnist hlutationovoi systemy antyoksydantnoho zakhystu orhanizmu buhaitsiv pry kadmiievomu navantazhenni. Visnyk Sumskoho natsionalnoho ahrarnoho universytetu. Seriia: Veterynarna medytsyna. 9, 70–73. Rezhym dostupu: http://nbuv.gov.ua/UJRN/ Vsna_vet_2013_9_22 (in Ukrainian).

Hutyi, B.V. (2013). Vplyv E-selenu na vmist vitaminiv A i E u krovi bychkiv za umov kadmiievoi intoksykatsii. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii im. Gzhytskoho, 15, 3(3), 311–314. Rezhym dostupu: http://nbuv.gov.ua/UJRN/nvlnu_2013_15_3(3)__55 (in Ukrainian).

Hutyi, B.V. (2013). Vplyv khlorydu kadmiiu u riznykh dozakh na aktyvnist aminotransferaz syrovatky krovi buhaitsiv. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii im. Gzhytskoho, 15, 1(1), 49–52. Rezhym dostupu: http://nbuv.gov.ua/UJRN/nvlnu_2013_15_1(1)__11 (in Ukrainian).

Hutyi, B.V. (2013). Vplyv khlorydu kadmiiu u toksychnykh dozakh na hlutationovu systemu antyoksydantnoho zakhystu orhanizmu bychkiv. Veterynarna biotekhnolohiia, 22, 112–116. Rezhym dostupu: http://nbuv.gov.ua/UJRN/vbtb_2013_22_23 (in Ukrainian).

Hutyi, B.V. (2013). Vplyv Meveselu na pokaznyky neenzymnoi systemy antyoksydantnoho zakhystu orhanizmu buhaitsiv za umov kadmiievoho navantazhennia. Biolohiia tvaryn, 15(3), 16–21. Rezhym dostupu: http://nbuv.gov.ua/UJRN/bitv_2013_15_3_4 (in Ukrainian).

Hutyi, B.V. (2013). Vplyv meveselu na vmist vitaminiv A i E u krovi bychkiv za umov kadmiievoi intoksykatsii. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii im. Gzhytskoho, 15, 3(1), 78–82. Rezhym dostupu: http://nbuv.gov.ua/UJRN/nvlnu_2013_15_3(1)__18 (in Ukrainian).

Hutyi, B.V. (2013). Vplyv meveselu ta E-selenu na riven pokaznykiv ne fermentnoi systemy antyoksydantnoho zakhystu orhanizmu buhaitsiv pry kadmiievomu navantazhenni. Veterynarna medytsyna, 97, 419–421. Rezhym dostupu: http://nbuv.gov.ua/UJRN/ vetmed_2013_97_172 (in Ukrainian).

Khariv, M., Gutyj, B., Butsyak, V., & Khariv, I. (2016). Hematological indices of rat organisms under condi-tions of oxidative stress and liposomal preparation ac-tion. Biological Bulletin of Bogdan Chmelnitskiy Meli-topol State Pedagogical University. 6 (1), 276–289. doi: 10.15421/201615.

Lavryshyn, Y.Y., Gutyj, B.V., Palyadichuk, O.R., & Vishchur, V.Y. (2018). Morphological blood indices of bulls in experimental chronic cadmium toxicosis. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 20(88), 108–114. doi: 10.32718/nvlvet8820.

Liu, J., Qian, S.Y., Guo, Q., Jiang, J., Waalkes, M.P., Mason, R.P., & Kadiiska, M.B. (2008). Cadmium generates reactive oxygen- and carbon-centered radicalspecies in rats: Insights from in vivo spin-trappingstudies. Free Radic Biol Med., 45(4), 475–481. doi: 10.1016/j.freeradbiomed.2008.04.041.

Lu, J., Jin, T., Nordberg, G., & Nordberg, M. (2005). Metal-lothionein gene expression in peripheral lympho-cytes and renal dysfunction in a population envi-ronmentally exposed to cadmium. Toxicol Appl Pharmacol 206(2), 150–156. doi: 10.1016/j.taap.2004.12.015.

Martyshuk, T.V., Gutyj, B.V., & Vishchur, O.I. (2016). Level of lipid peroxidation products in the blood of rats under the influence of oxidative stress and under the action of liposomal preparation of ‘‘Bu-taselmevit’’, Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6 (2), 22–27. doi: 10.15421/201631.

Nazaruk, N., Gutyj, B.V., & Hufriy, D. (2015). Influence of metifen and vitamix se on the activity of aminotransferases of bulls blood serum at cadmium nitrate loading. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 17(1), 121–126. Retrieved from https://nvlvet.com.ua/index.php/ journal/article/view/231.

Pavan Kumar, G., & Prasad, M.N.V. (2004). Cadmium-Inducible Proteins in Ceratophyllum demersum L. (a Fresh Water Macrophyte): Toxicity Bioassays and Relevance to Cadmium Detoxification. Bulletin of Environmental Contamination and Toxicology, 73(1), 174–181. doi: 10.1007/s00128-004-0410-4.

Peng, L., Wang, X., Huo, X., Xu, X., Lin, K., Zhang, J., Huang, Y., & Wu, K. (2015). Blood cadmium bur-den and the risk of nasopharyngeal carcinoma: a case–control study in Chinese Chaoshan population. Environmental Science and Pollution Research, 22(16), 12323–12331. doi: 10.1007/s11356-015-4533-4.

Rodríguez, E.M., Bigi, R., Medesani, D.A., Stella, V.S., Greco, L.S.L., Moreno, P.A.R., Monserrat, J.M., Pel-lerano, G.N., & Ansaldo, M. (2001). Acute and chronic effects of cadmium on blood homeostasis of an estu-arine crab, Chasmagnathus granulata, and the modi-fying effect of salinity. Brazilian Journal of Medical and Biological Research. 34(4), 509–518. https://www.ncbi.nlm.nih.gov/pubmed/11285463.

Salvatori, F., Talassi, CB, Salzgeber, S.A., Sipinosa, H.S., & Bernardi, M.M. (2004). Embryotoxic and long-term effects of cadmium exposure during embryogenesis in rats. Neurotoxicology and Teratology, 26(5), 673–680. doi: 10.1016/j.ntt.2004.05.001.

Uetani, M., Kobayashi, E., Suwazono, Y., Okubo, Y., Honda, R., Kido, T., & Nogawa, K. (2005). Selenium, Cadmium, Zinc, Copper, and Iron Concentrations in Heart and Aorta of Patients Exposed to Environmen-tal Cadmium. Bulletin of Environmental Contamina-tion and Toxicology, 75(2), 246–250. doi: 10.1007/s00128-005-0744-6.

Vlizlo, V.V. (2012). Laboratorni metody doslidzhen u biolohiyi, tvarynnytstvi ta veterynarniy medytsyni [Laboratory methods of investigation in biology, stock-breeding and veterinary]. Spolom, Lviv (in Ukrainian).

Abstract views: 0
PDF Downloads: 0
Published
2019-07-30
How to Cite
Lavryshyn, Y., & Gutyj, B. (2019). Protein synthesize function of bulls liver at experimental chronic cadmium toxicity. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 21(94), 92-96. https://doi.org/10.32718/nvlvet9417

Most read articles by the same author(s)

1 2 > >>