Nonspecific reactivity of the rabbits organism when exposed to cysticercosis


Keywords: phagocytic, lysozyme and bactericidal activity, CIC, cysticercosis invasion, Cysticercus pisiformis, rabbit.

Abstract

One of the actual rabbits breeding problem is the reduction of their resistance, which is caused by the spread of individual invasive diseases, especially cysticercosis, which is caused by the larval Cysticercus pisiformis stage of the mature cestode Taenia pisiformis. The study was conducted on 38 male rabbits age 34 months, weighing 3.54.0 kg of the Californian breed, selected by analogy. Animal were separated into two groups: healthy animals (control group) and sick animals (research group). After blood collection, slaughter was conducted and the presence of cysticercus cysts was visually determined in the organisms of the rabbits. Control animals received a balanced standard granular feed and water without limit; research group in addition to the standard granulated feed with water received flaccid hay. The animals were kept in mesh single-tier cages in the room, according to the current veterinary and sanitary standards. The definition of phagocytic activity of neutrophils was carried out with the addition of standardized to 2500000000/ml suspension of daily culture of E. coli 055K59№3912/41. The bactericidal activity of blood serum was determined by the method Smirnova A.V. and Kuzmina T.A. in relation to the E. coli microbial test-culture 055K59№3912/41. The serum lysozyme activity was determined by the Nephelometric method using the Dorofachuk V.G. method to the microbial test culture Micrococcus luteus ATSS9341. Circulating immune complexes were determined using polyethylene glycol in borate buffer (pH 8.4). It was found that in blood of sick animals phagocytic activity is lower than in blood of healthy ones (respectively, 41.82 ± 1.51% versus 47.38 ± 1.10%, P < 0.01). The main cells involved in phagocytosis are leukocytes, in particular neutrophils and eosinophils. A low indicator of phagocytic activity shows depressed phagocytosis in the organism of animals suffering from cysticercosis of rabbits. The phagocytic number in the blood of rabbits of the experimental group was significantly lower by 0.51 units (P < 0.05) as compared to the control group and correlated with the index of phagocytic activity. An important element of immunity are indicators of bactericidal and lysozyme activity of blood serum. Low bactericidal activity of serum by 12.88% (P < 0.01) and lysozyme activity in rabbits with Cysticercus pisiformis also indicates a weakening of the factors of nonspecific natural resistance of the organism. Analyzing the level of circulating immune complexes, we found a high level of medium (9.14 ± 0.40 vs 5.58 ± 0.50) and small (9.21 ± 1.35 vs 4.64 ± 0.68) CIC for cysticercosis, respectively, 1.64 (P < 0.01) and 1.98 times (P < 0.001) against the control. This indicates the inhibition of the immunobiological activity in the organism of rabbits as a result of the combination of specific antibodies with the products of the exchange of helminths.

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References

Blach-Olszewska, Z., & Jerzy, L. (2007). Mechanisms of over-activated innate immune system regulation in autoimmune and neurodegenerative disorders. Neuropsychiatr Dis Treat, 3(3), 365–372. https://www.ncbi.nlm.nih.gov/pubmed/19300567.

Das, A., Gupta, M.K., & Saxena, R.K. (2004). Enhanced activation of mouse NK cells by IL2 in the presence of circulating immune complexes. Сurrent science, 87(6), 780–783.

Dasso, J.F., & Howell, M.D. (1997). Neonatal appendectomy impairs mucosal immunity in rabbits. Cellular Immunology. 182(1), 29–37. doi: 10.1006/cimm.1997.1216.

Dorofejchuk, V.G. (1968). Lizocimnaja aktivnost' syvorotki krovi. Laboratornoe delo, 1, 28–34 (in Rus-sian).

Drouet-Viard, F.I., & Fortun-Lamothe, L. (2002). The organisation and functioning of the immune system. particular features of the rabbit. Review. World Rabbit Science. 10, 15–23. doi: 10.4995/wrs.2002.472.

Franci, O., Amici, A., Margarit, R., Merendino, N., & Piccolella, E. (1996). Influence of thermal and dietary stress on immune response of rabbits. Journal of Animal Science, 74(7), 1523–1529. doi: 10.2527/1996.7471523x.

Haitov, R.M., & Pinegin, B.V. (2005). Sovremennye predstavlenija o zashhite organizma protiv infekcii. Immunologija, 1, 61–64 (in Russian).

Jeklova, E., Leva, L., Kudlackova, H., & Faldyna, M. (2007). Functional development of immune response in rabbits. Veterinary Immunology and Immunopathology, 118(3–4), 221–228. doi: 10.1016/j.vetimm.2007.05.003.

Khariv, I., Gutyj, B., Hunchak, V., Slobodyuk, N., Vynyarska, A., Sobolta, A., Todoriuk, V., & Seniv, R. (2017). The influence of brovitatoxide in conjunction with milk thistle fruits on the immune system of turkeys for eimeriozic invasion. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 19(73), 163–168. doi: 10.15421/nvlvet7334.

Khariv, M., Gutyj, B., Ohorodnyk, N., Vishchur, O., Khariv, I., Solovodzinska, I., Mudrak, D., Grymak, C., & Bodnar, P. (2017). Activity of the T- and B-system of the cell immunity of animals under conditions of oxidation stress and effects of the liposomal drug. Ukrainian Journal of Ecology, 7(4), 536–541. doi: 10.15421/2017_157.

Khariv, M.I., & Hutyi, B.V. (2017). Dynamika fahot-sytarnoi aktyvnosti neitrofiliv u shchuriv za umov oksydatsiinoho stresu ta dii liposomalnoho preparatu. Biolohiia tvaryn, 19(1), 119–124. doi: 10.15407/animbiol19.01.119 (in Ukrainian).

Kosenko, M., Kotsiumbas, I., & Kosenko, Yu. (2004). Kontrol vplyvu veterynarnykh likarskykh zasobiv na stan imunitetu tvaryn. Veterynarna medytsyna, 1, 43–45 (in Ukrainian).

Lapovets, L.Ye., Lutsyk, B.D., Lebed, H.B., & Akimova, V.M. (2008). Posibnyk z laboratornoi imunolohii. Lviv (in Ukrainian).

Mannapova, R.R., & Podushkina, M.A. (2001). Imunna-ja sistema pushnyh zverej i krolikov. Sovremennye imunnomorfologicheskie problemy razvitija zhivotnyh pri associativnyh infekcionno-invazionnyh zabolevanijah i ispol'zovanie dlja ih profilaktiki bio-logicheski aktivnyh produktov pchelovodstva. M. Sbornik nauchnyh trudov, 296–311 (in Russian).

Maslianko, R.P. (1999). Osnovy imunolohii. Lviv (in Ukrainian).

Maslianko, R.P., & Kravtsiv, Yu.R. (2007). Funktsionalna aktyvnist neitrofilnykh hranulotsytiv u protyinfektsi-inomu zakhysti tvaryn. Naukovyi visnyk LNUVM ta BT. 9(33), 185–193 (in Ukrainian).

Maslianko, R.P., Kurtiak, B.M., & Pundiak, T.O. (2011). Imunorehuliatsiia v systemi mikroflora shlunkovo-kyshkovyi trakt. Naukovyi visnyk LNUVM ta BT imeni S.Z. Gzhytskoho, 13, 4(50), 268–275 (in Ukrainian).

Maslianko, R.P., Oleksiuk, I.I., & Padovskyi, A.I. (2001). Metodychni rekomendatsii dlia otsinky ta kontroliu imunnoho statusu tvaryn. vyznachennia faktoriv nespetsyfichnoi rezystentnosti, klitynnykh i humoral-nykh mekhanizmiv imunitetu proty infektsiinykh zakhvoriuvan. Lviv (in Ukrainian).

Parahonskij, A.P. (2006). Cirkulirujushhie immunnye kompleksy na pozdnih stadijah saharnogo diabeta. Sovremennye naukoemkie tehnologii, 1, 79–89 (in Russian).

Perederij, V.G., Zemskov, A.M., & Bychkov, N.G. (1995). Immunnyi status, principy ego ocenki j korrekcii im-munnyh narushenij. Kiev (in Russian).

Vlizlo, V.V., Fedoruk, R.S., & Ratych, I.B. (2012). La-boratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarnii medytsyni. Dovidnyk. za red. Vlizla, V.V. Lviv. SPOLOM (in Ukrainian).

Vishur, O.I., Hutyi, B.V., Hufrii, P.F., & Khariv, I.I. (2015). Imunnyi status, sposoby opinky i metopy korektsii u teliat rannoho viku. Monohrafiia. Lviv, SPODOM (in Ukrainian).

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Published
2019-07-30
How to Cite
Duda, Y. (2019). Nonspecific reactivity of the rabbits organism when exposed to cysticercosis. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 21(94), 132-135. https://doi.org/10.32718/nvlvet9424