State of the organism of the same year scaly carp infected by Eudiplozoon nipponicum


Keywords: the same year scaly carps, Eudiplozoon nipponicum, intensity and extensiveness of invasion, blood, hema-tological parameters, proteinblood composition, natural resistance, peroxide lipid oxidation, antioxidant system

Abstract

One of the least studied monogeneses doses, for today, there is diplopic zoonosis of carp. Previously, it was believed that there is only one fish parasitize bothrium – Diplozoon paradoxum. However, in recent years it has been discovered and described already more than 10 species of pathogens of diplozoons, in particular Eudiplozoon nipponicum. Outbreaks of this disease are increasingly beginning to be recorded in fish ponds farms of the western region of Ukraine. Moreover, more often than in others in the amount of this parasite occurs in young fish. In view of the above, the purpose of our research was to investigate the degree of infestation of the same year scaly carp with the mentioned ectoparasite and study the changes that occur in morphological and biochemical composition of blood, intensity of peroxide processes, as well as the state of antioxidant and immune systems in invasive fish. The research was carried out in the garden and fish farms of the State Enterprise “Rybhosp"Galitskyi” (renamed LLC “Rybgosp “Burshtynskyi”) Ivano-Frankivsk region and FG “Dobrotvir fish factory” of Lviv region with the same year scaly carp. Two experiments were selected for the experiment groups of the same yaer of scaly carp (control – non-invasive fish and experimental – invasive with Eudiplozoon nipponicum) in 6 specimens of fish in each group of weights bodies 45–47 g. In experimental fish they were studied their infestation with ectoparasites, as well hematological parameters, protein content of the blood, peroxide intensity processes, the state of antioxidant and immune systems of non-invasive and invasive specimens. During the parasitological examination of both experimental fish farms was detected infestation in scaly carp caused by ectoparasites Epidipalous nipponicum. In May there was a pick of infected the same year scaly carps (2.87 and 3.10 eggs/fish). Infected fish, compared to non-invasive fish, there were significant changes in hematological parameters. In particular, in the blood of parasites affected by fish the number of erythrocytes decreased (P < 0.001), the hemoglobin content (P < 0.001) and hematocrit and increased number of leucocyte (P < 0.001).The infected scaly carp the above-mentioned parasite pressed by the protein-synthesizing function of the organism, as indicated a high (P < 0.001) reduction in total protein, albumin and α-globulins in the blood serum, there is a depression in them humoral links of non-specific resistance and antioxidant system activity protection, and the content of products of peroxide oxidation of lipids in their hepatopancreas significantly increases.

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References

Bykhovskaya-Pavlovskaya, E.I. (1985). Parazity ryb. Rukovodstvo po izucheniyu. L.: Nauka (in Russian).

Davyidov, O.N., & Temnihanov, Yu.D. (2004). Bolezni presnovodnyih ryib. K.: Vetinform (in Russian).

Goncharov, S. (2019). The morphological changes in the blood of the predatory fish species with eustrongylidosis. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. Series: Veterinary sciences, 21(93), 15–20. doi: 10.32718/nvlvet9303.

Grynevych, N. (2018). Тhe dynamics of the physiological state changes and hematological indicators of the rainbow trout during the launch of the biofilter in the installations of the closed water utilization. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 20(83), 19–24. doi: 10.15421/nvlvet8304.

Kondrahin, I.P., Kurilova, N.V., & Malahov, A.G. (1985). Klinicheskaja laboratornaja diagnostika v veterinari: spravochnoe izdanie. M.: Agropromizdat (in Russian).

Vlizlo, V.V. (2012). Laboratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarnii medytsyni. Dovidnyk [za red. d. vet. n., prof., akademika NAAN V.V. Vlizla] Lviv, SPOLOM (іn Ukrainian).

Lakin, G.F. (1990). Biometrija. M.: Vysshaja shkola (in Russian).

Loboiko, Yu., Barylo, B., & Krushelnytska, O. (2017). Determination of the aminotransferase activity in tissues of infected with ectoparasites yearling carp. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 19(79), 17–21. doi: 10.15421/nvlvet7904.

Martyshuk, T.V., Gutyj, B.V., & Vishchur, O.I. (2016). Level of lipid peroxidation products in the blood of rats under the influence of oxidative stress and under the action of liposomal preparation of “Bu-taselmevit”. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6(2), 22–27. doi: 10.15421/201631.

Mauer, G. (1971). Disk-elektroforez. Teorija i praktika jelektroforeza v poliakrilamidnom gele. Moskva: Mir (in Russian).

Bauer, O.N. (1987). Opredelitel parazitov presnovodnyih ryib faunyi SSSR: v 3 t. pod red. O.N. Bauera. L.: Nauka, 2, 584 (in Russian).

Roudnický, P., Vorel, J., Ilgová, J., Benovics, M., Norek, A., Jedličková, L., Mikeš, L., Potěšil, D., Zdráhal, Z., Dvořák, J., Gelnar, M., & Kašný, M. (2018) Identification et caractérisation partielle d'un nouvelle serpine d’Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea). Parasite, 25, 61. doi: 10.1051/parasite/2018062.

Stybel, V., & Fedorovych, O. (2015) Histolohichna ta histokhimichna kharakterystyka kontaktnoi adhezii “parazyt-khaziain” pry monohenoidozakh koropa. Tvarynnytstvo Ukrainy, 5, 34–38 (іn Ukrainian).

Tushnytska, N.Y. (2009) Stan imunnoi systemy i metabolichnyi profil krovi koropa pry zakhvoriuvanniakh krasnukhoiu i riznykh sposobakh yoho likuvannia. PhD [thesis]. Lviv (іn Ukrainian).

Valigurová, A., Hodová, I., Sonnek, R., Koubková, B., & Gelnar, M. (2011) Eudiplozoon nipponicum in focus: monogenean exhibiting a highly specialized adaptation for ectoparasitic lifestyle. Parasitol Res., 108(2), 383–394. doi: 10.1007/s00436-010-2077-6.

Zurawski, T.H., Mousley, A., Mair, G. R., Brennan, G. P., Maule, A. G., Gelnar, M., & Halton, D.W. (2001). Immunomicroscopical observations on the nervous system of adult Eudiplozoon nipponicum (Monogenea: Diplozoidae). International Journal for Parasitology, 31(8), 783–792. doi: 10.1016/S0020-7519(01)00192-8.

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Published
2019-07-30
How to Cite
Fedorovych, O., & Gutyj, B. (2019). State of the organism of the same year scaly carp infected by Eudiplozoon nipponicum. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 21(94), 146-151. https://doi.org/10.32718/nvlvet9427

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