Effect of “Amprolinsyl” and “Amprolium 22%” on morphological indices of blood of turkeys for eumeria invasion


Keywords: Eymeriosis, turkeys, amprolium 22%, Amprolinsyl, hematologic indices

Abstract

The article gives data on the influence of Amprolinsyl and Amprolium 22% on morphological parameters of blood after eumirioic invasion. It has been shown that after application of amprolium 22% for the treatment of turkeys infected with eumeria invasion, gradual normalization of hematological parameters of blood begins. Thus, at the 3rd day of treatment, the number of red blood cells increased, but remained 25.1% lower, and the hemoglobin level increased, but remained at 16.3% lower, compared with clinically healthy poultry. During this period, within the limits of normal values was the value of the color index, the mass of hemoglobin in the erythrocyte and the value of hematocrit. The obtained results indicate a gradual normalization of the erythropoietic function of the bone marrow. However, the large average volume of one erythrocyte is 1.12 ± 0.07 μm against 0.96 ± 0.03 μm³ in the control, and the decrease in the average concentration of hemoglobin in one erythrocyte by 13.3% against the norm indicates an incomplete recovery of the erythropoietic bone marrow function. At day 5 of treatment with amprolium, 22% of red blood cells and hemoglobin levels increased but still remained below physiological values by 12.3% and 6.8%, respectively, compared to control. At the 10th day of the experiment, restoration of hematopoietic function of the bone marrow was observed on physiological values of erythrocytes and hemoglobin content of the blood, as well as blood leukogram. In the treatment of turkeys with "Amprolisyl", at the 5th day, the hematopoietic function of the bone marrow completely normalized, as indicated by the number of red blood cells, hemoglobin content and red blood cell index values. It is known that the values of red blood cells, namely the volume of erythrocytes, the mass and concentration of hemoglobin in them, and the color index objectively reflect the state of hematopoietic function of the bone marrow. The results of our studies indicate that in the turkey infected with imeiriosis and treated with amprolium 22%, clinical recovery occurs at day 5, and the hematopoietic function of the bone marrow is restored spontaneously in 5 days after recovery. In the treatment of turkeys with Amprolinsyl, the restoration of morphological parameters, the normalization of white blood cell leukemogram and the clinical recovery of turkeys arose on the fifth day of treatment. Thus, due to the fact that Amporolinsyl, in addition to the active ingredient, contains sparse fruit of thistle spots, which contain high levels of vitamins A and K, and trace elements – Ferum, Kuprum and Cobalt, which are directly involved in hemopoiesis, therefore, the restoration of hematopoietic function of the bone marrow comes much faster than with the application of amprolium itself 22%.

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References

Batakov, E.A. (2001). Effect of Silibum marianum oil and legalon on lipid peroxidation and liver anti-oxydant systems in rats intoxicated with carbon tetra-chloride. Eksp. Klin. Farmacol., 64(4), 53–55. https://www.ncbi.nlm.nih.gov/pubmed/11589112.

Bohach, M.V., & Taranenko, I.L. (2003). Parazytarni khvoroby indykiv fermerskykh i prysadybnykh hospodarstv pivdnia Ukrainy. Ahrarnyi visnyk Prychornomorۥia, 21, 311–317 (in Ukrainian).

Chekman, I.S., Pohotova, H.A., Nebesna, T.Iu., & Horchakova N.O. (2014). Kvantovo-farmakolohichne doslidzhennia antyoksydantnykh vlastyvostei sylimarynu. Ukrainskyi biofarmatsevtychnyi zhurnal, 2, 24–28. http://nbuv.gov.ua/UJRN/ubfj_2014_2_7 (іn Ukrainian).

Golubtsova, M.V., Stybel, V.V., & Sobolta, A.G. (2018). Influence of disinfectant “Bi-des” on the sporulation on eimery oocyst of chicken. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 20(87), 70–73. doi: 10.15421/nvlvet8714.

Gutyj, B., Hariv, I., Gunchak, V., Sobolta, A., Prijma, O., & Iesina, E. (2018). The influence of “Amprolinsile” and brovitacoccide on the activity of blood serum enzymes by the eumeriosic invasion. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 20(83), 51–55. doi: 10.15421/nvlvet8310.

Gutyj, B., Khariv, I., Binkevych, V., Binkevych, O., Levkivska, N., Levkivskyj, D., & Vavrysevich, Y. (2017). Research on acute and chronic toxity of the experimental drug Аmprolinsyl. Regulatory Mechanisms in Biosystems, 8(1), 41–45. doi: 10.15421/021708.

Gutyj, B., Martyshchuk, T., Bushueva, I., Semeniv, B., Parchenko, V., Kaplaushenko, A., Magrelo, N., Hirkovyy, A., Musiy, L., & Murska, S. (2017). Morphological and biochemical indicators of blood of rats poisoned by carbon tetrachloride and subject to action of liposomal preparation. Regulatory Mechanisms in Biosystems, 8(2), 304–309. doi: 10.15421/021748.

Hariv, M.I., & Gutyj, B.V. (2016). Influence of the liposomal preparation Butaintervite on protein synthesis function in the livers of rats under the influence of carbon tetrachloride poisoning. Visnyk of Dnipropetrovsk University. Biology, medicine, 7(2), 123–126. doi: 10.15421/021622.

Hikino, H., Kiso, G., Wagner, I., & Fiebig, M. (1984). Antihepatotoxic actions of flavonolignans from Silybum marianum fruits. Planta Medica, 50(2), 248–250. doi: 10.1055/s-2007-969690.

Hirkovyi, A.Iu., & Stybel, V.V. (2014). Vplyv imunizatsii na leikotsytarnyi profil ta biokhimichni pokaznyky krovi kurchat, invazovanykh zbudnykamy eimeriozu. Naukovo-tekhnichnyi biuleten Instytutu biolohii tvaryn i Derzhavnoho naukovo-doslidnoho kontrolnoho instytutu vetpreparativ ta kormovykh dobavok, 15(4), 184–187. http://nbuv.gov.ua/UJRN/ Ntbibt_2014_15_4_37 (in Ukrainian).

Hunchak, A.V., Ratych, I.B., Gutyj, B.V., & Paskevych, H.A. (2016). Metabolichna diia yodu v orhanizmi ptytsi za yoho nestachi abo nadlyshku v ratsioni. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii imeni S. Z. Gzhytskoho, 18, 2(67), 70– 76. doi: 10.15421/nvlvet6716 (in Ukrainian).

Khariv, M., & Gutyj, B. (2017). Dynamics of phagocytic activity of neutrophils in rats under oxidative stress and the action of liposomal preparation. The Animal Biology, 19(1), 119–124. doi: 10.15407/ animbiol19.01.119 (in Ukrainian).

Khariv, M., Gutyj, B., Butsyak, V., & Khariv, I. (2016). Hematological indices of rat organisms under conditions of oxidative stress and liposomal preparation action. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6(1), 276–289. doi: 10.15421/201615.

Khariv, M., Gutyj, B., Ohorodnyk, N., Vishchur, O., Khariv, I., Solovodzinska, I., Mudrak, D., Grymak, C., & Bodnar, P. (2017). Activity of the T- and B-system of the cell immunity of animals under conditions of oxidation stress and effects of the liposomal drug. Ukrainian Journal of Ecology, 7(4), 536–541. doi: 10.15421/2017_157.

Khariv, I.I. (2012). Biloksyntyzuvalna funktsiia pechinky v intaktnykh indykiv na tli dii brovitakoktsydu i plodiv roztoropshi pliamystoi. Naukovo-tekhnichnyi biuleten Instytutu biolohii tvaryn i Derzhavnoho naukovo-doslidnoho kontrolnoho instytutu vetpreparativ ta kormovykh dobavok, 13(3–4), 258– 262 (in Ukrainian).

Khariv, M.I., Hutyi, B.V., Vishchur, O.I., & Solovodzinska, I.Ye. (2016). Funktsionalnyi stan pechinky u shchuriv za umov oksydatsiinoho stresu ta dii liposomalnoho preparatu. Naukovi zapysky Ternopilskoho natsionalnoho pedahohichnoho universytetu, 2(66), 76–84 (in Ukrainian).

Kryshtalska, M., Hunchak, V., & Gutyj, B. (2017). Influence of the drug “Trifuzol” on the functional state of the liver in chickens for eymeriozic invasion. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 19(77), 76–79. doi: 10.15421/nvlvet7718.

Martyshuk, T.V., Gutyj, B.V., & Vishchur, O.I. (2016). Level of lipid peroxidation products in the blood of rats under the influence of oxidative stress and under the action of liposomal preparation of “Butaselmevit”, Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6(2), 22–27. doi: 10.15421/201631.

Martyshuk, T.V., Vishchur, O.I., & Hutyi, B.V. (2016). Stan hlutationovoi lanky antyoksydantnoi systemy u krovi shchuriv za umov oksydatsiinoho stresu ta za dii liposomalnoho preparatu “Butaselmevit”. Naukovyi visnyk natsionalnoho universytetu bioresursiv i pryrodokorystuvannia Ukrainy, 234, 135–144 (in Ukrainian).

Pentiuk, N.O. (2010). Antyfibrozna aktyvnist sylimarynu, kandesartanu ta foliievoi kysloty u khvorykh na khronichni hepatyty. Visnyk problem biolohii i medytsyny, 4, 124–130. http://nbuv.gov.ua/UJRN/ Vpbm_2010_4_31 (іn Ukrainian).

Semenko, O.V. (2014). Deiaki osoblyvosti poshyrennia ta zakhodiv borotby z eimeriozom ptytsi. Suchasne ptakhivnytstvo, 8, 7–11. http://nbuv.gov.ua/UJRN/ Sps_2014_8_4 (іn Ukrainian).

Shaker, E., Mahmoud, H., & Mnaa, S. (2010). Silymarin, the antioxidant component and Silybum marianum extracts prevent liver damage. Food and Chemical Toxicology, 48(3), 803–806. doi: 10.1016/j.fct.2009.12.011.

Skottova, N., & Klecman, V. (1998). Silimarin as a potential hypocholes terolaemic drug. Physiological Research, 47(1), 1–7.

Zhao, J., & Agarwan, R. (1999). Tissue distribution of silibinin, the major active constituent of silimarin, in mice and its association with enhancement of phase II enzymes: implication in cancer chemoprevention. Carcinogenesis, 20(11), 2101–2108. doi: 10.1093/carcin/20.11.2101.

Нu, J.N., Fuller, L., & Dougald, L.R. (2000). Anticoccidials interfere with development of protective immunity agains coccidiosis in broilers. J. Appl. Poult., 9(3), 352–358. doi: 10.1093/japr/9.3.352.

Vlizlo, V.V. (2012). Laboratorni metody doslidzhen u biolohiyi, tvarynnytstvi ta veterynarniy medytsyni [Laboratory methods of investigation in biology, stock-breeding and veterinary]. Spolom, Lviv (in Ukrainian).

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Published
2019-07-30
How to Cite
Stybel, V., Gutyj, B., Hariv, I., Slivinska, L., & Prijma, O. (2019). Effect of “Amprolinsyl” and “Amprolium 22%” on morphological indices of blood of turkeys for eumeria invasion. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 21(94), 157-162. https://doi.org/10.32718/nvlvet9429

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