Chronic toxicity of the Iron (IV) clathrochelate complexes for white rats


Keywords: hexahydrazide clathrochelate, toxicology, body weight, morphological parameters of blood, biochemical parameters of blood serum

Abstract

The article presents the results of studies of chronic toxicity of the Iron in the rare unconventional valence IV. During long-term use of the Iron(IV) clathrochelate complexes for white rats we have established the dynamics of the body weight of rats, the relative coefficients of mass of the internal organs, the content of hemoglobin and morphological parameters of blood, biochemical parameters of blood serum of animals of this species. The daily drinking of solution of the Iron(IV) clathrochelate complexes at the doses of 500 and 1000 mg/kg b. w. resulted in a decrease in body weight, an increase in the relative indices of masses of liver and kidney, and a decrease in the relative indices of masses of  spleen and heart on 30th day. The hemoglobin content in the blood of rats of the experimental group was less than the control indicator by 3–47% (P < 0.05), which is evidence of inhibition of its synthesis under the influence of the Iron (IV) clathrochelatе. Changes in the morphological composition of the blood were characterized by marked leukocytopenia. The use of rats of a solution of the Iron (IV) clathrochelate complexes at doses of 500 and 1000 mg/kg b. w. caused the development of hypoproteinemia, hypercreatinemia and hyperurinemia. Iron(IV) clathrochelate complexes reduced alaninaminotransferase activity in the serum of rats of both experimental groups by 1580% (P < 0.05); aspartataminotransferase activity increased significantly by day 10 and decreased by day 30; the activity of alkaline phosphatase was independent of the doses of the drug during the experimental period. The content of Calcium total, Phosphor inorganic and Iron in the serum of rats of the experimental groups was at the level of indicators in the animals of the control group. Consequently, comprehensive studies of the effects of solution of the Iron (IV) clathrochelate complexes at doses of 500 and 1000 mg/kg b. w. were performed for the first time with long-term administration to white rats, which revealed the main patterns of metabolic disorders and physiological functions.

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References

Dukhnitsky, V.B., Derkach, I.M., Plutenko, M.O., Fritsky, I.O., & Derkach, S.S. (2018). Vyznachennja paramet-riv gostroi toksychnosti ferumu (IV) na bilyh myshah. Ukrainian Journal of Ecology, 8(2), 308–312. doi: 10.15421/2018_343 (in Ukrainian).

Dukhnitsky, V.B., Derkach, I.M., Plutenko, M.O., Fritsky, I.O., & Derkach, S.S. (2019). Cumulative properties of Iron(IV) clathrochelate in rats. Visnyk PDAA, 2, 238−246.

Derkach, I. (2017). Suchasni tendencii na vitchyznjano-mu rynku ferumvmisnyh preparativ dlja tvaryn. Naukovyj visnyk Lvivskogo nacionalnogo univer-sytetu veterynarnoi medycyny ta biotehnologij imeni S.Z. Gzhyckogo, 19(78), 23–25. doi: 10.15421/nvlvet7805 (in Ukrainian).

Commission of the European Communities: Council Directive of 18 December 1986 on the Lows, regulat-ing the Application of Principles of Good Laboratory Practice and the Verification of Their Applications for Tests on Chemical Substances (87/18/EEC) (1991). The Rules Governing Medicinal Products in the Euro-pean Community, 1, 145–146.

Cui, J., Li, Y., Yu, P., Zhan, Q., Wang, J., Chi, Y., & Wang, P. (2018). A novel low molecular weight Enteromorpha polysaccharide-iron (III) complex and its effect on rats with iron deficiency anemia (IDA). International journal of biological macromolecules, 108, 412–418. doi: 10.1016/j.ijbiomac.2017.12.033.

England, J. Bigelow, О., Katherine, M., Heuvelen, V., Farquhar, E., Martinho, M., Meier, K., Frisch, J., Münck, E., & Que, L. (2014). An ultra-stable oxoiron (IV) complex and its blue conjugate base. Chemical Science, 3, 1204–1215. doi: 10.1039/C3SC52755G.

Groves, J.T. (2006). High-valent iron in chemical and biological oxidations. Journal of Inorganic Biochemis-try, 100(4), 434−447. doi: 10.1016/j.jinorgbio.2006.01.012.

Kim, J.C., Wilcock, P., & Bedford, M.R. (2018). Iron sta-tus of piglets and impact of phytase superdosing on iron physiology: A review. Animal Feed

Science and Technology, 235, 8–14. doi: 10.1016/j.anifeedsci.2017.11.001.

Kotsiumbas, I.Ya. (2006). Doklinichni doslidzhennja veterynarnyh likars'kyh zasobiv. L'viv. Triada pljus (in Ukrainian).

Lipiński, P., Starzyński, R., Canonne-Hergaux, F., Tudek, B., Oliński, R., Kowalczyk, P., Dziaman, T., Thibaudeau, O., Gralak, M.A., Smuda, E., Woliński, J., Usińska, A., & Zabielski, R. (2010). Benefits and Risks of Iron Supplementation in Anemic Neonatal Pigs. American journal of hematology, 177(3), 1233–1243. doi: 10.2353/ajpath.2010.091020.

Maes, D., Steyaert, M., Vanderhaeghe, C., López Rodríguez, A., de Jong, E., Del Pozo Sacristán, R., Vangroenweghe, F., & Dewulf, J. (2011). Comparison of oral versus parenteral iron supplementation on the health and productivity of piglets. Veterinary record, 168, 188. doi: 10.1136/vr.c7033.

Streyl, K., Carlstron, J., Dantos, E., Mendoza, R., Islas, J.A., & Bhushan, C. (2015). Field Evaluation of the Effectiveness of an Oral Toltrazuril and Iron Combi-nation (Baycox® Iron) in Maintaining Weaning Weight by Preventing Coccidiosis and Anaemia in Neonatal Piglets, 114(1), 193–200. doi: 10.1007/s00436-015-4525-9.

Todoriuk, V.B., Hunchak, V.M., Gutyj, B.V., Gufriy, D.F., Hariv, I.I., Khomyk, R.I., & Vasiv, R.O. (2018). Pre-clinical research of the experimental preparation “Ferosel T”. Ukrainian Journal of Veterinary and Ag-ricultural Sciences, 1(1), 3−9. doi: 10.15421/ujvas1-1.01.

Tomyn, S., Shylin, S.I., Bykov, D., Ksenofontov, V., Gumienna-Kontecka, E., Bon, V., & Fritsky, I.O. (2017) Indefinitely stable iron (IV) cage complexes formed in water by air oxidation. Nature Communications, 8, 1−8. doi: 10.1038/ncomms14099.

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Published
2019-11-02
How to Cite
Dukhnitsky, V., Derkach, I., Derkach, S., Fritsky, I., & Plutenko, M. (2019). Chronic toxicity of the Iron (IV) clathrochelate complexes for white rats. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 21(95), 15-21. https://doi.org/10.32718/nvlvet9503