Influence of the preparation “Bendamin” on the indicators of antioxidant protection of rat myocardium in experimental modeling of heart failure
The aim of this study was to investigate the effect of Bendamin on the antioxidant status of rats in experimental doxorubicin-induced cardiomyopathy. The studies were performed on white sexually mature young male of Vistar line rats weighing 180–200 g, which were kept on a standard diet of the Institute Vivarium of the State Research Institute of Veterinary Drugs and Feed Additives. Animals were divided into 3 groups of 6 animals in each: control group – intact animals; experimental group R1, in which animals were simulated with doxorubicin-induced cardiomyopathy by intraperitoneal administration of doxorubicin at a dose of 2.5 mg/kg 3 times a week for two weeks; experimental group R2, in which animals were injected with the drug “Bendamin” at a dose of 20 mg/kg after injection of doxorubicin. The data obtained indicate that the introduction into the experimental animals of the experimental group of doxorubicin is accompanied by intensification in the myocardium of the processes of free radical oxidation. The increase in diene conjugates was found to be 32.7% and TBK-active products increased by 37.6% compared to the control group of rats. Doxorubicin was also found to cause inhibition of the glutathione system of the animal's antioxidant protection after administration to rats. After administration of the drug “Bendamin”, rats of the second experimental group were suppressed with lipid peroxidation under the conditions of doxorubicin intoxication. In the rats of the second experimental group, the level of intermediates and end products is likely to decrease, so in the rat myocardial homogenate the level of diene conjugates decreased by 16.8% and the level of TBA-active products by 20.8% compared to the first experimental group. who had clinical signs of cardiomyopathy caused by doxorubicin administration. The use of the drug “Bendamin” in rats of the second experimental group contributed to the enhancement of the enzymatic and non-enzymatic element of the antioxidant system, protecting the structural and functional integrity of cell bioembranes. The results of the studies enrich the pharmacological characteristics of the drug “Bendamin”, indicate its sufficiently clear protective effect on the myocardium in experimental doxorubicin cardiomyopathy and is a convincing proof of the feasibility of the drug in veterinary practice.
Oldach, M.S., Ueda, Y., Ontiveros, E.S., Fousse, S.L., Harris, S.P., & Stern, J.A. (2019). Cardiac Effects of a Single Dose of Pimobendan in Cats With Hypertrophic Cardiomyopathy; A Randomized, Placebo-Controlled, Crossover Study. Front Vet Sci., 6, 15. doi: 10.3389/fvets.2019.00015.
Tjostheim, S.S., Kellihan, H.B., Grint, K.A., & Stepien, R.L. (2019). Effect of sildenafil and pimobendan on intracardiac heartworm infections in four dogs. J Vet Cardiol., 23, 96–103. doi: 10.1016/j.jvc.2019.02.001.
Undhad, V.V., Fefar, D.T., Jivani, B.M., Gupta, H., Ghodasara, D.J., Joshi, B.P. & Prajapati, K.S. (2012). Cardiac troponin: an emerging cardiac biomarker in animal health. Vet. World, 5, 508–511. doi: 10.5455/vetworld.2012.508-511.
Varcholyak, I.S., & Gutyi, B.V. (2019). Determination of the chronic toxicity of preparation “Bendamin” on laboratory animals. Theoretical and Applied Veteri-nary Medicine, 7(2), 63‒68. doi: 10.32819/2019.71011.
Varkholiak, I.S. (2016). The usage of medicines at the cardiovascular pathologies in dogs and cats. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 18, 3(71), 261–265. https://nvlvet.com.ua/index.php/ journal/article/view/974.
Varkholiak, I.S., & Gutyj, B.V. (2018). Determination of acute toxicity of “Bendamin” drug in laboratory animals. Scientific Messenger of Lviv National Uni-versity of Veterinary Medicine and Biotechnologies, 20(92), 209–212. doi: 10.32718/nvlvet9243.
Varkholiak, I.S., & Gutyj, B.V. (2019). The degree of cumulation of the “Bendamin” drug in the body of white rats. Scientific Messenger of Lviv National Uni-versity of Veterinary Medicine and Biotechnologies. Series: Veterinary sciences, 21(94), 82–85. doi: 10.32718/nvlvet9415.
Vlizlo, V.V., Fedoruk, R.S., & Raty`ch, I.B. (2012). Laboratorni metody` doslidzhen` u biologiyi, tvary`nny`cztvi ta vetery`narnij medy`cy`ni: dovidny`k. L`viv: Spolom (in Ukrainian).
Yata, M., Kooistra, H.S., & Beijerink, N.J. (2019). Cardiorenal and endocrine effects of synthetic canine BNP1-32 in dogs with compensated congestive heart failure caused by myxomatous mitral valve disease. J Vet Intern Med., 33(2), 462–470. doi: 10.1111/jvim.15416.
Zhulikova, O.A. (2016). Monitoring rasprostranenija serdechno-sosudistyh zabolevanij sredi koshek i sobak v g. Blagoveshhensk amurskoj oblasti. Dal'-nevostochnyj agrarnyj vestnik, 2(38), 49–56. https://cyberleninka.ru/article/n/monitoring-rasprostraneniya-serdechno-sosudistyh-zabolevaniy-sredi-koshek-i-sobak-v-g-blagoveschensk-amurskoy-oblasti (in Russian).
Abstract views: 121 PDF Downloads: 75