The influence of long-term drinking of citric acid on the organism of F0 female rats
The influence of long-term drinking of Citric acid on the organism of female rats was studied by the investigation of morphological and biochemical indices and organ mass coefficients. The study was performed on 10 laboratory female rats F0, which were organized in groups (5 – control, 5 – experimental) 2–2.5 months aged, with body mass 110–120 g. The animals of the experimental group received aqueous solution of Citric acid (С6Н12О6 х Н2О, “ch.p.”) in the dose 0.8 mg/kg b. m. during the physiologic and puberty periods, fertilization and pregnancy. Animals of both groups had constant access to the drinking water. The received results of studies of red cells in the experimental group are comparative to the control group animals indicate the tendency to decrease the number of red blood cells by 6%, hemoglobin in the blood on 22.7%, average hemoglobin concentration in hemoglobin by 25%, average hemoglobin content in the erythrocyte on 20.9%, average hemoglobin volume on 4% against the background of an increase of hematocrit value on 5.4%. The experimental data obtained indicate a probable increase in the number of leukocytes in the blood of the experimental group of animals on 42.4%, and a tendency for a 2-folf increase in eosinophils. The results of biochemical studies of serum indicate minor differences between serum iron content (increase on 5.6%) in experimental and control animals groups. Also, the received results indicate the tendency of an increase by 3.3% of serum trasferrin on the background of decrease of total (by 3.6%) and unsaturated (by 7.6%) Fe-binding ability of blood. Animals receiving Citric acid found a tendency to increase, in terms of indicators of ALT activity – by 18.9% and alkaline phosphatase by 13.2% comparing with the control group of animals. The blood of female rats showed significantly higher urea level of 72.5% and a lower creatinine level of 19.6%. The content of average molecular weight substances in the serum of female rats of the experimental group under the influence of Citric acid is less than in animals of the control group on 2.8%. Studies of the individual organs status with the determination of their weight and mass coefficients revealed that against a background to the coefficients of the control group of female rats the weight of the liver increased likely by 16.1%. In the future, we plan to investigate the effect of long-term drinking of Citric acid on the organism of F1 rats.
Dmytruk, I.V. (2008). Riststymuliuiucha ta antystresova diia lymonnoi i burshtynovoi kyslot ta probiotyku “Probiol-L” v hodivli molodniaku svynei: avtoref. dys. na zdobuttia naukovoho stupenia kand. s-h. nauk: spets. 06.02.02 “Hodivlia tvaryn i tekhnolohiia kormiv”. Lviv; Lvivskyi nats. universytet veterynar-noi medytsyny ta biotekhnolohii imeni S.Z. Gzhytskoho (in Ukrainian).
EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed) (2015). Scientific Opinion on the safety and efficacy of citric acid when used as a technological additive (acidity regulator) for all animal species. EFSA Journal 2015, 13(2), 4010. doi: 10.2903/j.efsa.2015.4010.
European convention for the protection of vertebrate animals used for experim. and other scientific purpos-es (1986). Coun. of Europe, Strasbourg, 53. https://rm.coe.int/168007a67b.
Fales, V.M., Khivrych, O.V., & Lytvynenko, A.M. (2009). Analiz vyrobnytstva ta zastosuvannia lymonnoi kysloty. Kharchova promyslovist, 8, 91–94 (in Ukrainian).
Gabrijeljan, N.I., Levickij, Je.R., & Dmitriev, A.A. (1985). Skriningovyj metod opredelenija srednih molekul v biologicheskih zhidkostjah: [metod. rekomendacii]. M. (in Russian).
Kondrahin, I.P., Kurilov, N.V., Malahov, A.G., Arhipov, V.A., Belov, A.D., Beljakov, I.M., Blinov, N.I., Korob-ov, A.V., Frolova, L.A., & Sevast'janova, N.A. (1985). Klinicheskaja laboratornaja diagnostika v veterinarii. M.: Agropromizdat (in Russian).
Kostenko, V.M., Dmytruk, I.V., & Nechyporuk, Yu.I. (2004). Produktyvnist ta hematolohichni pokaznyky teliat ta porosiat pry zghodovuvanni yim lymonnoi ta yantarnoi kyslot. Naukovyi visnyk LNAVM im. S.Z. Hzhytskoho, 6, 72–79 (in Ukrainian).
Kostenko, V.M., Dmytruk, I.V., Nechyporuk, Yu.I., & Sukhovukha, S.M. (2008). Efektyvnist vykorystannia lymonnoi ta burshtynovoi kyslot, probiotykiv ta yikh sumishok, yak riststymuliuiuchykh ta imunozakhystnykh bezpechnykh dobavok v hodivli tvaryn. Zbirnyk naukovykh prats VDAU, 34, 230–233 (in Ukrainian).
Kotsiumbas, I.Ya. (2006). Doklinichni doslidzhennia veterynarnykh likarskykh zasobiv: Dovidnyk. Lviv: Triada plius (in Ukrainian).
Nagoba, B.S., Wadher, B.J., & Selkar, S.P. (2011). Citric Acid Treatment of Chronic Wounds in Animals. In-ternational Journal of Animal and Veterinary Ad-vances, 3(1), 26–28. https://pdfs.semanticscholar.org/ dbff/abfbecf42bfb3f1bd7f19ab048c1934cbcd0.pdf.
Novinjuk, L.V. (2009). Citraty – bezopasnye nutrienty. Pishhevye ingredienty: syr'e, dobavki, 1, 70–71 (in Russian).
Ostapyuk, A.Y., & Gutyj, B.V. (2019). Influence of cad-mium sulfate at different doses on the functional state of the liver of laying chicken. Scientific Messen-ger of Lviv National University of Veterinary Medi-cine and Biotechnologies. Series: Veterinary sciences, 21(94), 103–108. doi: 10.32718/nvlvet9419.
Rylskyi, O.F., & Sherstoboieva, O.V. (2010). Lymonna kyslota – chynnyk zberezhennia bioriznomanittia prokariot za umov zabrudnennia. Ahroekolohichnyi zhurnal, 1, 56–60 (in Ukrainian).
Sakhanda, I.V. (2014). Preparaty Hermaniiu ta yikh zastosuvannia v medytsyni. Ukrainskyi naukovo-medychnyi molodizhnyi zhurnal, 4(84), 83–86. http://nbuv.gov.ua/UJRN/Unmmj_2014_4_19 (in Ukrainian).
Tesarivska, U.I., Fedoruk, R.S., & Shumska, M.I. (2016). Reproduktyvna funktsiia samok shchuriv F1 i postna-talnyi rozvytok shchureniat F2 za dii riznykh doz na-nohermaniiu tsytratu. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii imeni S.Z. Gzhytskoho, 18(3), 124–130. doi: 10.15421/nvlvet7128 (in Ukrainian).
Tesarivskaja, U.I., Fedoruk, R.S., Hrabko, M.I., & Shumskaja, M.V. (2016). Jembrional'naja toksichnost' i zhiznesposobnost' priploda samok krys pri vypaivanii im limonnoj kisloty. Collection of works of Scientific Symposium with International Par-ticipation “Zootechnycal science – an important fac-tor for the european type of the agriculture”. Maxi-movca, 726–730 (in Russian).
Wright, E., & Hughes, R.E. (1976). Some effects of dietary citric acid in small animals. Food and Cosmetics Toxicology, 14(6), 561–564. doi: 10.1016/S0015-6264(76)80009-0.
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