Parameters of chronic toxicity of the solution for intrauterine use “Yodozol”
The study of the chronic toxicity of the solution for intrauterine use in the form of aerosol – “Yodosol”, is a mandatory stage of preclinical study of the drug, which is a new development of PE «Biopharm» and the Experimental Station of epizootology of the Institute of Infectious Diseases of NAAN. In a scientific experiment on laboratory animals, it is possible to evaluate the safety of the drug at different times of use, to determine the area of toxic action and doses that do not cause adverse health effects. Therefore, the purpose of the study was to study the chronic toxicity of the drug “Yodosol”, in particular the determination of tolerant and toxic doses for rodents. The experiments were carried out in accordance with the requirements for biomedical research, the selection of analogues for testing and control, ensuring the same conditions of feeding and retention, as well as accounting for results. “Yodosol” is a light yellow foamy liquid with 1 ml containing 5 mg iodine and 10 mg potassium iodide. The drug is used for the prevention and treatment of postpartum intrauterine infections in cows, pigs, sheep and goats (endometritis, pyrometers, cervicitis, vaginitis, delayed litter caused by iodine-sensitive microorganisms), after delivery of rhododerma, caesarean delivery and caesarean delivery. The drug has antimicrobial, anti-inflammatory and analgesic effects, improves proliferative processes in the tissues of the genital organs, helps to reduce the time of recovery of animals. The drug is used according to the guideline, after its livestock products are used without restrictions. No significant deviations in the behavior of the experimental animals were observed in the studies of chronic toxicity of the drug “Yodosol”. Visually noted only a slight inhibition of the rats of the experimental group, which received a 10-fold overdose. According to biochemical studies, in the group, compared with the control animals, there was a slight deviation of the indicators of protein metabolism and activity of hepatospecific enzymes: growth of total protein, alkaline phosphatase and aspartateaminotransferase. However, such changes in the blood parameters of the experimental animals were short-lived and restorative, characterizing the study drug as low-toxic. All studies conducted to determine the parameters of chronic toxicity of the solution for intrauterine use of “Yodosol” included in the registration materials of the medicinal product.
Gutyj, B.V. (2014). Doslidzhennia toksychnosti preparatu “Mevesel-iniektsiinyi” v khronichnomu doslidi [Study of toxicity of the drug “Mevsel-injection” in the chronic experiment] Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii imeni S.Z. Gzhytskoho – Scientific Bulletin of the S.Z. Gzhytskiy Lviv National Universi-ty of Veterinary Medicine and Biotechnology, 16, 2(59), 214–217 (in Ukrainian).
Katsaraba, O.A., Stefanyk, V.Y., Kostyshyn, Ye.Ye., Sachuk, R.N., & Kulinich, O.V. (2018). Application of intrauterine aerosol preparation for the treatment of cows with postpartum complications. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. 20(87), 55–59. doi: 10.15421/nvlvet8711.
Kosenko, M.V., Malik, O.G., & Kotsyumbas, I.Ya. (1997). Toksikologіchnij kontrol' novih zasobіv zahistu tvarin: metodichnі rekomendacіi [Poison control of new meansof protecting animals: guidelines]. Kiev (in Ukrainian).
Kotsyumbas, I.Ya., Malik, O.G., & Patereha I.P. et al. (2006). Doklіnіchnі doslіdzhennja veterinarnih lіkars'-kih zasobіv [Preclinical studies of veterinary medicinal products]. Lviv: Triadplus (in Ukrainian).
Lakin, H.F. (1990). Biometria [Biometrics]. Moskow: Vuszaichkola (in Russian).
Rozyn, D.H. (1964). Sovremennaia otsenka toksychnosty khlorproyzvodnukh uhlerodov zhyrnoho riada po heksenalovomu testu na belukh myshakh Farmakolohyia і toksykolohyia. [Current toxicity assessment of fatty chlorine derivatives of hexagonal test in white mice], 5, 613–614 (in Ukraini-an).
Rylova M.L. (1964). Metodu yssledovanyia khronycheskoho deistvyia vrednukh faktorov sredu v eksperymente [in Russian].
Sachuk, R., Kulinich, O., & Katsaraba, O. (2017). Eefficiency of the aerosol intrauterine preparation “Metrazol-bio” in endometritis in cows. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 19(82), 110–113. doi: 10.15421/nvlvet8223.
Sachuk, R.M., Zhigaliuk, S.V., Tverdiy, V.M., Stravsky, Ya.S., Katyukha, S.M., Katsaraba, O.A. et al. (2018). Aerozolnyi preparat sefalosporynovoho riadu dlia profilaktyky ta likuvannia pisliarodovykh vnutrish-nomatkovykh infektsii u tvaryn [Aerosol preparation of cephalosporin series for the prevention and treat-ment of postpartum intrauterine infections in ani-mals]. Ukraine Utility model patent No. 127501 (in Ukrainian).
Stravsky, Y.S. (2017). Diahnostyka, likuvannia ta profil-aktyka akusherskoi patolohii u koriv (metodychni rekomendatsii) [Diagnosis, treatment and prevention of obstetric pathology in cows (guidelines)]. Lviv (in Ukrainian).
Todoriuk, V.B., Hunchak, V.M., Gutyj, B.V., Gufriy, D.F., Hariv, I.I., Khomyk, R.I., & Vasiv, R.O. (2018). Pre-clinical research of the experimental preparation “Ferosel T”. Ukrainian Journal of Veterinary and Ag-ricultural Sciences, 1(1), 3–9. doi: 10.32718/ujvas1-1.01
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