Innovative domestic product of “Емкарвак” – vaccine against blacleg at the market of veterinary immunobiological facilities


  • P. Boyko Institute of Veterinary Medicine of NAAS, Kyiv, Ukraine https://orcid.org/0000-0001-6022-1874
  • B. Busol National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”, Kharkov, Ukraine
  • L. Akymenko State Scientific Control Institute of Biotechnology and Microorganism, Kyiv, Ukraine
  • O. Boyko Experimental Station of Epizootology of the Institute of Veterinary Medicine of the National Academy of Sciences of Ukraine, Rivne, Ukraine https://orcid.org/0000-0003-4552-5348
  • B. Kurtiak Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnology Lviv, Ukraine http://orcid.org/0000-0001-8401-5928
  • O. Assory Sumy biological state factory, Sumy, Ukraine
Keywords: Clostridium chauvoe, blacleg, local strain, constructing of vaccine, immunogenicity, protective capacity, virulence

Abstract

The results over the test of new domestic vaccine against to the blackleg – “ЕМКАРВАК” was  brought. The local productive strain C. chauvoei of Л– 7 has high immunogenicity, protective  capacity and  create  immunity duration over 9 months. The corresponding changes in regulation technological process was enable to use  of the newest data in relation to immunogenicity capacity of C. chauvoei. In particular, duration of accumulation of microbial mass of C. chauvoei in a reactor diminished from 36–42 hours to 16–18 hour, as an adsorbent is used aerosils of brand of А-300 (a production is Germany) from the calculation of 3 ± 0.1mg/sm3 culture; the supernatants was recovered by concentration of liquid after inactivation. The microbial mass of vaccine is 3.5 ± 0.3×1093. The tests of vaccine of “ЕМКАРВАК” showed that preparation was sterile, inactivated, harmless (a bioassay is on guinea-pigs), owns high antigen (the level of antibodies for the instilled animals presented 1:828 ± 257 on a 21th day after inoculation) and immunogenicity activity. The field tests of vaccine of “ЕМКАРВАК” showed  that a vaccine owns high antigenicity, providing even in 12 months after her inoculation  high level of specific antibodies (the level for the instilled animals presented 1:32 ± 6.0 against 1:11 ± 3.6 for control animals). The productive tests of the productive-experienced series of vaccine of  “ЕМКАРВАК’ conducted in the conditions of 22 unhappy economies of the Volyn’ area on young cattle. Rejections of general (oppression, increase of temperature, loss of appetite and others like that) or changes of local character (slight swelling in place inoculation, increase of regional lymphatic knots) it was not educed. Thus, the vaccine of “ЕМКАРВАК” in the conditions of the field test appeared harmless and immunogenicity, able to support tense immunity at the receptive contingent of cattle during 9 months (term of supervision).

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Published
2019-12-14
How to Cite
Boyko, P., Busol, B., Akymenko, L., Boyko, O., Kurtiak, B., & Assory, O. (2019). Innovative domestic product of “Емкарвак” – vaccine against blacleg at the market of veterinary immunobiological facilities. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 21(96), 19-27. https://doi.org/10.32718/nvlvet9604