Influence of medication “Membranostabil” on expression of immunoreceptor proteins in small intestine of ruminants during the period of formation of colostral immunity
The influence of medication “Membranostabil” on the indexes of content of proteins with MM of 33, 40, and 47 kDa in the plasmolemma of enterocytes of jejunum of new-born calves during the period from birth till the age of 24-hours was investigated. The research was conducted on calves (males) of Ukrainian black-and-white breed during the spring. Throughout the trial period, the calves of the control and experimental groups were kept on a standard diet for newborn calves, colostrum. In addition, calves of the experimental group got the medication “Membranostabil” based on the soybean lecithin. Proteins of plasmolemma of enterocytes of the jejunum of calves were isolated from the membrane fraction of lysates and examined by electrophoresis. Electrophoretic separation of proteins of plasmolemma of the jejunum enterocytes of newborn calves revealed proteins with MM of 37, 40 and 43 kDa on electrophoregram. The data of expression of immunoreceptor proteins with MM of 37, 40, 43 kDa and the dynamics of their changes depending on the time after birth in calves of the control and experimental groups were analyzed. The decrease in the total content of expression of proteins with MM of 37, 40, and 43 kDa in the membrane fraction of lysates of enterocytes of the jejunum of calves at the age of 6 hours compared to the newborn ones was demonstrated. The levels of expression of each immunoreceptor protein are considered with their comparative analysis between the individual fractions in the cytolemma of the enterocytes of the jejunum of newborn calves, which according to their molecular weights correspond to MM isoforms of FcγRIII/CD32. The data obtained showed a significantly higher expression of level of proteins with molecular mass of 37 kDa on the apical membrane of enterocytes, compared with proteins with MM of 40 and 43 kDa, both at birth of calves and at the age of 6 and 24 hours. Based on the data obtained, proteins with MM of 37, 40, and 43 kDa of plasmolemma of the jejunum enterocytes undergo significant changes in the process of postnatal ontogeny in cattle. These changes indicate the synthesis of these proteins in the enterocytes themselves in the process of formation of colostral immunity in newborn calves under the influence of the medication “Membranostabil” used by this research group, compared with calves of the control group. It is shown that the application of medication “Membranostabil” for the new-born calves activates the transport of immunoglobulins of colostrum in the intestine of these animals, assists the increasing of level of colostral immunity, and prevents the occurrence of digestive disorders. Findings of this research correlate with the results of clinical observation in newborn calves.
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