Prevalence of the nematodes Eustrongylides exisus Jägerskiöld, 1909, – larvae (Nematoda: Dioctophymatidae) infection in the Rutilis rutilis, linnaeus 1758 and the seasonal dynamics of the infection in the waters of the Dnipro-Buh estuari in southern Ukrai

Keywords: Eustrongylides excisus, roach, prevalence, seasonal dynamics.


In this study, we determined the prevalence and seasonal dynamic of the infection of the Rutilis rutilis, Lіnnaeus 1758 with the nematode Eustrongylides excisus. The fish was caught in the waters of the Dnipro-Buh estuary in seven sample collection sites between 2016 and 2019. They all underwent ichthyopathological examination. Parasites, found during examination, undergone microscopy to determine their taxonomy. Overall 595 specimens were obtained. The mean prevalence of infection over entire study period (2016–2019) was 17.4 %. Ichthyopathological examination of the roach caught in the waters of the Dnipro-Buh estuary revealed the infection with larvae E. excises nematode. The parasitic larvae were found in the muscles of the ventral abdominal wall of the roach. There was more than two-fold difference in the prevalence of infection between sample collection sites – from 12 % in site 5 near Sofiivka to 28 % in site 3 near the village Oleksandrivka over the study period in 2016–2019. The total prevalence of infection in roaches in the Dnipro-Buh estuary was 17.4 %. Seasonal changes were not statistically significant and ranged from 12.8 % in summer to 20.1 % in spring. Annual changes suggested a possible upward trend, but more observations are needed to confirm it. Аnnual changes were not statistically significant. The prevalence of infection changed throughout the year: there were two peaks in spring (20.1 %) and autumn (18.3 %). In summer (12.8 %) and winter (15.1 %) the prevalence of infection was lower. Morphologically, the E. excisus larvae found in roaches were different compared to those found in predatory fishes: they had more pale color and were smaller in length. E. excisus is a prevalent infection of roach in Dnipro-Buh estuary. Considering seemingly increasing prevalence of E.excisus infection, further studies are required to understand a pathophysiology of E.excisus infection  in mammals and humans, since data remain limited.


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Bauer, O. (1987). The keys to the freshwater fish para-sites. Nauka, Moscow. V. 3 (in Russian).

Barros, L. A., Tortelly, R., Pinto, R. M., & Gomes, D. C. (2004). Effects of experimental infections with larvae of Eustrongylides ignotus Jäegerskiold, 1909 and Contracaecum multipapillatum (Drasche, 1882) Baylis, 1920 in rabbits. Arq. Bras. Med. Vet. Zootec., 56(3), 2004. doi: 10.1590/S0102-09352004000300007.

Bikhovskaya-Pavlovskaya, I. E. (1985). Parasitesof fish: Study Guide. Nauka, Leningrad (in Russian).

Branciari, R., Ranucci, D., Miraglia, D., Valiani, A., Vero-nesi, F., & Urbani, E. (2016). Occurrence of parasites of the genus Eustrongylides spp. (Nematoda: Diocto-phymatidae) in fish caught in Trasimeno lake, Italy. Italian Jour. of Food Safety, 5(4), 6130. doi: 10.4081/ijfs.2016.6130.

Chernova, T. N. (1975). Seasonal Changes in the Parasitofauna of Pike and Roach of the Lakes Paleostomi and Dzhapan. Proceedings of the All-Union Scientific Research Institute of Fisheries and Oceanography. Volume CV, 108–120 (in Russian).

Deardorff, T. L., & Oversreet, R. M. (1991). Seafood-transmitted zoonoses in the United States: the fishes, the dishes, and the worms. In: Microbiology of Marine Food Products (ed. by D.R. Ward & C.R. Hackney), New York, 211–265.

Fedorov, N. M., Firsov, N. F., & Soloviev, N. A. (2014). Veterinary and sanitary examination in river perch with Eustrongylidosis. Veterinary pathology, 3–4, 68–73 (in Russian).

Honcharov, S. L., Soroka, N. M., Pashkevich, I. Y., Duboviy, A. O., & Bondar, A. O. (2018). Infection of Predatory Fish with Larvae of Eustrongylides excisus (Nematoda, Dioctophymatidae) in the Delta of the Dnipro River and the Dnipro-Buh Estuary in Southern Ukraine. Vestnik Zoologii, 52(2), 137–144. doi: 10.2478/vzoo-2018-0015.

Guerin, P. F., Marapendi, S., Grail, S. L. (1982). Intestinal perforation caused by larval Eustrongylides. Morb. Mort. Week. Rep., 31, 383–389.

Korniychuk, Y. M., Pronkina, N. V., & Belofastova, I. P. (2008). Nematode Fauna of the round goby Apollo-nia (Neogobius) melanostomus in the Black Sea and Sea of Azov. Ecology of the sea. 76, 17–22. 4811.

Köse, S. (2010). Evaluation of Seafood Safety Health Hazards for Traditional Fish Products: Preventive Measures and Monitoring Issues. Turk J. Fish Aquat Sci., 10(1), 139–160. doi: 10.4194/trjfas.2010.0120.

Lichtenfels, J. R., & Stroup, C. F. (1985). Eustrongylides sp. (Nematoda: Dioctophymatoidea): First Report of an Invertebrate Host (Oligochaeta: Tubificidae) in North America. Proc. Helminthol. Soc. Wash, 52(2), 320–323. 0018-0130/52/320.pdf.

Ljubojevica, D., Novakov, N., Djordjevicc, V., Rados-avljevicd, V., Pelica, M., & Cirkovic, M. (2015). Poten-tial parasitic hazards for humans in fish meat. Proce-dia Food Science, 5, 172–175. doi: 10.1016/j.profoo.2015.09.049.

Melo, F. T., Melo, C. S., & Nacimento, L. C. (2015). Mor-phological characterization of Eustrongylides sp. Lar-vae (Nematoda, Dioctophymatoidea) parasite of Rhinella marina (Amphibia: Bufonidae) from Eastern Amazonia. Braz. J. Vet. Parasitol., 25(2), 7–12. doi: 10.1590/S1984-29612016024.

Metin, S., Diden, B. I., & Boyci, Y. O. (2014). Occurrence of Eustrongylides excisus, Jägerskiöld, 1909 – larvae (Nematoda: Dioctophymatidae) in Pikeperch (Sander lucioperca) in Lake Egirdir. Egirdir Su Ṻrṻnleri Facṻltesi Dergisi, 10(1), 20–24.

Moshu, A. (2014). Helminths of fish ponds between the rivers Dniester and Prut, potentially dangerous to hu-man health. Kishineu: Eco-Tiras (in Russian).

Narr, L. L., O’Donell, J. G., Libster, B., Alessi, P., & Abra-ham, D. (1996). Eustrongylidiasis – a parasitic infection acquired by eating live minnows. J. Am Ost Assoc., 96(7), 400–402.

Noei, M. R., Ibragimov, S., & Sattari, M. (2015). Parasitic worms of the Persian sturgeon, Acipenser persicus Bo-rodin, 1897 from the southwestern shores of the Cas-pian Sea. Iranian Jour. of Ichthyology, 2(4), 287–295.

Novakov, N., Bjelic-Čabrilo, O., Ćircovic, M., Jubojevic, D., & Lujic, J. (2013). Eustrongylidosis of European Catfish (Siluris glanis). Bulg. J. Agric. Sci., 19(1), 72–76.

Pazooki, J., Masoumian, M., Yahyazadeh, M., & Abbasi, J. (2007) Metazoan Parasites from Freshwater Fishes of Northwest Iran. J. Agric. Sci. Technol., 9, 25–33.

Sinyaeva, D. M. (2014). Potentially dangerous fish nema-todes in our region: Modern problems of teaching and research in biology at Ukrainian universities. Materials of the First All-Ukrainian Scientific and Practical Con-ference young scientists and students with interna-tional participation, October 8–9, 2014, Dneprope-trovsk, Ukraine, 170–172 (in Ukrainian).

Shirazian, D., Schiller E. L., Glaser, C. A., & Vonderfecht, S. L. (1984). Pathology of larval Eustrongylides in the rabbit. J. Parasitol., 70(5), 803–806.

Soylu, E. (2013). Metazoan Parasites of Perch Perca fluviatilis L. From Lake Sığırcı, Ipsala, Turkey. Paki-stan J. Zool., 45(1), 2013, 47–52.

Spalding, M. G., & Forrester, D. J. (1993). Pathgenesis of Eustrongylides ignotus (Nematoda: Diocto-phymatidae) in Ciconiiformes, Journal of Wildlife Diseases, 29(2), 250–260. doi: 10.7589/0090-3558-29.2.250.

Wittner, M., Turner, J. W., Jacquette, G., Ash, L. R., Salgo, M. P., & Tanovitz, H. B. (1989). Eustrongylidiasis – a parasitic infection acquired by eating sushi. New Engl. J. Med. 320, 1124–1126. doi: 10.1056/NEJM198904273201706.

Yesipova, N. B. (2013). The spread of parasitic nema-todes in fish Eustrongylides excisus Zaporozhye (Dnipro) reservoir. Modern probl. of theor. and pract. ichthyology: materials VI International Ichthyological Scien. and Pract. Conf. Ternopil., 86–88 (in Russian).

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Honcharov, S. (2020). Prevalence of the nematodes Eustrongylides exisus Jägerskiöld, 1909, – larvae (Nematoda: Dioctophymatidae) infection in the Rutilis rutilis, linnaeus 1758 and the seasonal dynamics of the infection in the waters of the Dnipro-Buh estuari in southern Ukrai. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 22(97), 31-38.