Effect of sodium selenite and feed additive “Metisevit plus” on morphological parameters of blood of rats at the intoxication of Cadmium and Lead


Keywords: toxicology, Cadmium, Lead, sodium selenite, feed additive “Metisevit plus”

Abstract

Heavy metals, Lead and Cadmium, are hazardous environmental pollutants. In the body of mammals, Cadmium and Lead have a toxic effect on a number of organs and systems. The mechanism of action of these poisons is to inhibit the activity of enzyme systems by blocking carboxyl, amine and SH groups of protein molecules. The aim of the study was to investigate the effect of sodium selenite and feed additive Metisevit plus on the morphological parameters of rat blood by Cadmium and Lead intoxication. The experiments were carried out on 200–220 g male Wistar rats, out of which 3 groups of animals were formed: a control and two experimental animals. Rats in the control and experimental groups were administered 16.6 % aqueous lead acetate at a dose of 100 mg/kg and 0.029 % aqueous cadmium chloride at a dose of 2.0 mg/kg. The rats of the first experimental group additionally used sodium selenite. The rats of the second experimental group additionally used the feed additive Metisevit plus. Lead-cadmium loading in rats was accompanied by erythrocytopenia and leukopenia. The lowest erythrocyte count was in the blood of diseased rats at 21 days, where it decreased by 41.3 %, respectively, compared to the initial values. It was found that hemoglobin level decreased to 90.3 ± 2.63 g/l during Cadmium and Lead intoxication in control rats. At the same time as the decrease in hemoglobin level and the number of erythrocytes in intoxicated rats, an increase in the average hemoglobin content in one erythrocyte of blood was observed up to 20.57 ± 1.20 pg As a result of the development of lead-cadmium intoxication in ill rats, leukopenia was noted, which, in our opinion, is associated with systemic lesions of cadmium and lead in the blood-forming organs of animals that suppress leukopoiesis. It was found that the number of leukocytes in patients of rats of group C decreased by 36.1 % compared with the initial data. The use of sodium selenite and the feed additive Metisevit plus in rats under lead-cadmium loading contributed to the increase in the number of red blood cells and hemoglobin level, as well as the decrease in the number of leukocytes in their blood. The improvement of normalizing effect on the morphological parameters of blood of rats under conditions of loading with heavy metals was shown by the feed additive Metisevit plus in comparison with sodium selenite.

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References

Al-Azemi, M., Omu, F. E., Kehinde, E. O., Anim, J. T., Oriowo, M. A., & Omu, A. E. (2010). Lithium protects against toxic effects of cadmium in the rat testes. J. Assist. Reprod. Genet, 27(8), 469–476. doi: 10.1007/s10815-010-9426-3.

Ali, M. M., Murthy, R. C., & Chandra, S. V. (1986). De-velopmental and longterm neurobehavioral toxicity of low–level in utero Cd exposure in rats. Neurobe-havioral Toxicology and Teratology, 8(5), 463–468. https://www.ncbi.nlm.nih.gov/pubmed/3785508.

El-Shahat, A. E., Gabr, A., Meki, A. R., & Mehana, E. S. (2009). Altered testicular morphology and oxidative stress induced by cadmium in experimental rats and protective effect of simultaneous green tea extract. Int. J. Morphol., 27(3), 757–764. doi: 10.4067/S0717-95022009000300020.

Gutyi, B., Ostapiuk, A., Kachmar, N., Stadnytska, O., Sobolev, O., Binkevych, V., Petryshak, R., Petryshak, O., Kulyaba, O., Naumyuk, A., Nedashkivsky, V., Nedashkivska, N., Magrelo, N., Golodyuk, I., Naza-ruk, N., & Binkevych, O. (2019). The effect of cadmi-um loading on protein synthesis function and func-tional state of laying hens’ liver. Ukrainian Journal of Ecology, 9(3), 221–226. doi: 10.15421/2019_733.

Gutyj, B. V., Gufriy, D. F., Binkevych, V. Y., Vasiv, R. O., Demus, N. V., Leskiv, K. Y., Binkevych, O. M., & Pavliv, O. V. (2018). Influence of cadmium loading on glutathione system of antioxidant protection of the bullocks’bodies. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnolo-gies, 20(92), 34–40. doi: 10.32718/nvlvet9207.

Hutyi, B. V. (2013). Vplyv Meveselu na pokaznyky neenzymnoi systemy antyoksydantnoho zakhystu orhanizmu buhaitsiv za umov kadmiievoho navanta-zhennia. Biolohiia tvaryn, 15(3), 16–21. Rezhym dostupu: http://nbuv.gov.ua/UJRN/bitv_2013_15_3_4 (in Ukrainian).

Hutyi, B. V. (2013). Vplyv meveselu na vmist vitaminiv A i E u krovi bychkiv za umov kadmiievoi intoksykatsii. Naukovyi visnyk Lvivskoho natsional-noho universytetu veterynarnoi medytsyny ta bio-tekhnolohii im. Gzhytskoho, 15, 3(1), 78–82. Rezhym dostupu: http://nbuv.gov.ua/UJRN/nvlnu_2013_15_3(1)__18 (in Ukrainian).

Hutyi, B. V. (2013). Vplyv meveselu ta E-selenu na riven pokaznykiv ne fermentnoi systemy antyoksydant-noho zakhystu orhanizmu buhaitsiv pry kadmiievo-mu navantazhenni. Veterynarna medytsyna, 97, 419–421. Rezhym dostupu: http://nbuv.gov.ua/UJRN/vetmed _2013_97_172 (in Ukrainian).

Hutyi, B. V. (2015). Aktyvnist systemy antyoksydant-noho zakhystu orhanizmu bychkiv za hostroho kad-miievoho toksykozu. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii im. Gzhytskoho, 17, 1(1), 31–36. Rezhym dostupu: http://nbuv.gov.ua/UJRN/nvlnu_ 2015_17_1(1)__8 (in Ukrainian).

Hutyi, B. V., Slobodian, S. O., & Kurylas, L. V. (2019). TU U 10.9 – 00492990-018:2019. Kormova dobavka “Metisevit plius” (in Ukrainian).

Kuras, L. D., & Ersteniuk, H. M. (2019). Pokaznyky ener-hetychnoho obminu v sertsevii tkanyni eksperymen-talnykh tvaryn za umov vplyvu kadmii khlorydu. Medychna ta klinichna khimiia, 21(1), 25–31. Rezhym dostupu: http://nbuv.gov.ua/UJRN/ Medkh_2019_21_1_6 (in Ukrainian).

Lavryshyn, Y. Y., & Gutyj, B. V. (2019). Protein synthe-size function of bulls liver at experimental chronic cadmium toxicity. Scientific Messenger of Lviv Na-tional University of Veterinary Medicine and Biotech-nologies. Series: Veterinary sciences, 21(94), 92–96. doi: 10.32718/nvlvet9417.

Lavryshyn, Y. Y., Gutyj, B. V., Palyadichuk, O. R., & Vishchur, V. Y. (2018). Morphological blood indices of bulls in experimental chronic cadmium toxicosis. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 20(88), 108–114. doi: 10.32718/nvlvet8820.

Lavryshyn, Y. Y., Gutyj, B. V., Paziuk, I. S., Levkivska, N. D., Romanovych, M. S., Drach, M. P., & Lisnyak, O. I. (2019). The effect of cadmium loading on the activity of the enzyme link of the glutathione system of bull organism. Scientific Messenger of Lviv Na-tional University of Veterinary Medicine and Biotech-nologies. Series: Veterinary sciences, 21(95), 107–111. doi: 10.32718/nvlvet9520.

Marushko, Yu. V., Tarynska, O. L., Olefir, T. I., & Fus, S. V. (2010). Kadmii: nakopychennia ta vplyv na orhanizm dytyny. Medychna nauka Ukrainy, 3, 62–67. Rezhym dostupu: http://nbuv.gov.ua/UJRN/nvnmu_ 2010_3_12 (in Ukrainian).

Myslyva, T. M. (2013). Svynets i kadmii u hruntakh ahrolandshaftiv Zhytomyrskoho Polissia. Visnyk Sumskoho natsionalnoho ahrarnoho universytetu. Se-riia: Ahronomiia i biolohiia, 3, 43–50. Rezhym dostupu: http://nbuv.gov.ua/UJRN/Vsna_agro_2013 _3_13 (in Ukrainian).

Myslyva, T. M. (2013). Svynets i kadmii u hruntakh pryrodnykh i ahrolandshaftiv Zhytomyrskoho Polissia. Visnyk Zhytomyrskoho natsionalnoho ahroekolohichnoho universytetu, 1(1), 36–49. Rezhym dostupu: http://nbuv.gov.ua/UJRN/Vzhnau_2013_1(1)__8 (in Ukrainian).

Nazaruk, N., Gutyj, B., & Hufriy, D. (2015). Influence of metifen and vitamix se on the activity of aminotrans-ferases of bulls blood serum at cadmium nitrate load-ing. Scientific Messenger of LNU of Veterinary Medi-cine and Biotechnologies, 17(1), 121–126. Retrieved from https://nvlvet.com.ua/index.php/journal/article/ view/231.

Ostapyuk, A. Y., & Gutyj, B. V. (2018). Influence of cadmium loading on morphological parameters of blood of the Laying Hens. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 20(88), 48–52. doi: 10.32718/nvlvet8808.

Ostapyuk, A. Y., & Gutyj, B. V. (2019). Influence of cadmium sulfate at different doses on the functional state of the liver of laying chicken. Scientific Messen-ger of Lviv National University of Veterinary Medi-cine and Biotechnologies. Series: Veterinary sciences, 21(94), 103–108. doi: 10.32718/nvlvet9419.

Petrynych, V. V., Vlasyk, L. I., & Petrynych, O. A. (2017). Svynets: toksykolohichni, hihiienichni ta biolohichni aspekty. Klinichna ta eksperymentalna patolohiia, 16(2), 97–102. Rezhym dostupu: http://nbuv.gov.ua/UJRN/kep_2017_16_2_22 (in Ukrainian).

Rodríguez, E. M., Bigi, R., Medesani, D. A., Stella, V. S., Greco, L. S. L., Moreno, P. A. R., Monserrat, J. M., Pel-lerano, G. N., & Ansaldo, M. (2001). Acute and chron-ic effects of cadmium on blood homeostasis of an es-tuarine crab, Chasmagnathus granulata, and the modifying effect of salinity. Brazilian Journal of Med-ical and Biological Research, 34(4), 509–518. https://www.ncbi.nlm.nih.gov/pubmed/11285463.

Sachko, R. G., Lesyk, Ja. V., Luchka, I. V., & Nevostruyeva, I. V. (2016). Contents of heavy metals in food, organism and animal products in the Zacar-pathian biogeochemical province. Scientific Messen-ger LNUVMBT named after S. Z. Gzhytskyj, 18, 3(71), 87–90. doi: 10.15421/nvlvet7120.

Salvatori, F., Talassi, CB, Salzgeber, S. A., Sipinosa, H. S., & Bernardi, M. M. (2004). Embryotoxic and long-term effects of cadmium exposure during embryo-genesis in rats. Neurotoxicology and Teratology, 26(5), 673–680. doi: 10.1016/j.ntt.2004.05.001.

Stepanchuk, V. V. (2014). Ontohenetychni osoblyvosti tsyrkadiannykh khronorytmiv vilnoradykalnoho ho-meostazu za umov svyntsevoho otruiennia. Klinichna ta eksperymentalna patolohiia, 13(2), 131–133. Rezhym dostupu: http://nbuv.gov.ua/UJRN/kep_2014 _13_2_33 (in Ukrainian).

Uetani, M., Kobayashi, E., Suwazono, Y., Okubo, Y., Honda, R., Kido, T., & Nogawa, K. (2005). Selenium, Cadmium, Zinc, Copper, and Iron Concentrations in Heart and Aorta of Patients Exposed to Environmen-tal Cadmium. Bulletin of Environmental Contamina-tion and Toxicology, 75(2), 246–250. doi: 10.1007/s00128-005-0744-6.

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Published
2020-05-07
How to Cite
Slobodian, S., Gutyj, B., & Murska, S. (2020). Effect of sodium selenite and feed additive “Metisevit plus” on morphological parameters of blood of rats at the intoxication of Cadmium and Lead. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 22(97), 52-57. https://doi.org/10.32718/nvlvet9710

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