Investigation of the antimicrobial activity of danofoloxacin against bacteria pathogens in goats
Fluoroquinolones are critical antimicrobials for both human and veterinary medicine. Due to their unique mechanism of antimicrobial action and good pharmacokinetic properties, they are often the first choice drugs in the treatment of bacterial infections in animals. The purpose of the investigation was to study the antimicrobial activity of a third-generation fluoroquinolone antibiotic of danofloxacin against bacteria, pathogens of respiratory and intestinal infection in goats. The samples of the nasal outflows (respiratory infection) and fecal masses (intestinal infection) were collected from clinically ill goats for microbiological studies. The sensitivity test of the microflora of the biomaterial, carried out by the disco-diffusion method, showed that the microorganisms of all the samples were sensitive to danofloxacin. Bacteria Streptococcus pneumonia (n = 10), Staphylococcus aureus (n = 4) and Escherichia coli (n = 2) were isolated and identified from nasal exudate samples (n = 10). Pathogenic strains of Escherichia coli were isolated from all faecal samples (n = 12). The degree of bacteriostatic activity of danofloxacin was determined by establishing its minimum inhibitory concentration (MIC) for bacterial isolates by sequential dilutions in a liquid nutrient medium. The average MIC of danofloxacin for Streptococcus pneumoniae isolates was 0.26 ± 0.13 μg/ml and for Staphylococcus aureus isolates – 0.25 ± 0.075 μg/ml. For Escherichia coli strains isolated from faeces of goats suffering from coli infection, the average MIC of danofloxacin was 0.38 ± 0.12 μg/ml (range 0.2 to 0.8 μg/ml). Antimicrobial sensitivity testing have shown a high level of bacteriostatic activity of danofloxacin against bacteria, pathogens of respiratory and intestinal infections in goats. This may be the argument for the use of danofloxacin-based chemotherapeutic agents in the treatment of bacterial infections in goats, especially for the empirical approach to therapy.
Boothe, D. M. (2006). Principles of antimicrobial therapy. Vet Clin North Am Small Anim Pract, 36(5), 1003-47. doi: 10.1016/j.cvsm.2006.07.002.
Clifford, K., Desai, D., Prazeres da Costa, C., Meyer, H., Klohe, K., Winkler, A. S., Rahman, T., Islame, T., & Zamana, M. H. (2018). Antimicrobial resistance in livestock and poor quality veterinary medicines. Bull World Health Organ, 96(9), 662–664. doi: 10.2471/BLT.18.209585.
Cruz, A. D., Lopes C. A. D. M., Modolo, J. R., & Gottschalk, A. F. (1997). Comparative ''in vitro'' study on the susceptibility and emergence of mutants resistant to danofloxacin and ciprofloxacin among Staphylococcus aureus isolated from bovine mastitis. Revista de Microbiologia. São Paulo, Soc Brasileira Microbiologia, 28(1), 61–64. http://hdl.handle.net/11449/32806.
Duhamel, G. E., Moxley, R. A., Maddox, C. W., & Erickson, E. D. (1992). Enteric infection of a goat with enterohemorrhagic Escherichia coli. J Vet Diagn Invest, 4(2), 197–200. doi: 10.1177/104063879200400218.
Grobbel, M., Lubke-Becker, A., Wieler, L. H., Froyman, R., Friederichs, S., & Filios, S. (2007). Comparative quantification of the in vitro activity of veterinary fluoroquinolones. Vet Microbiol, 124(1–2), 73–81. doi: 10.1016/j.vetmic.2007.03.017.
Hooper, D. C., & Wolfson, J. S. (1993). Mechanism of quinolone action and bacterial killing. In “Quinolone Antimicrobial Agents”. Amer. Soc. For Microbiol., Washington, 482–512.
Marín, P., Escudero, E., Fernández-Varón, E., Cárceles, C. M., Corrales, J.C., Gómez-Martín, A., Martínez, I. (2010). Short communication: Fluoroquinolone susceptibility of Staphylococcus aureus strains isolated from caprine clinical mastitis in southeast Spain. J Dairy Sci, 93(11), 5243–5245. doi: 10.3168/jds.2010-3345.
National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals, informational supplement. (2004). NCCLS document M31-S1 (ISBN 1- 56238-534-8), p. 12.
Oros, J., Fernandez, A., Rodriguez, J. L., & Poveda, J. B. (1997). Bacteria associated with enzootic pneumonia in goats. Journal of Veterinary Medicine series B, 44(1-10), 99–104. doi: 10.1111/j.1439-0450.1997.tb00955.x.
Ozawa, M., Baba, K., Shimizu, Y., & Asai, T. (2010). Comparison of in vitro activities and pharmacokinetics/pharmacodynamics estimations of veterinary fluoroquinolones against avian pathogenic Escherichia coli isolates. Microb. Drug Resist, 16, 327–332. doi: 10.1089/mdr.2010.0024.
Paphitou, N. I. (2013). Antimicrobial resistance: action to combat the rising microbial challenges. Int J Antimicrob Agents, 42, 25–28. doi: 10.1016/j.ijantimicag.2013.04.007.
Resolution No. XXVIII. OIE LIST OF ANTIMICROBIAL AGENTS OF VETERINARY IMPORTANCE, adopted by International Committee of OIE on 24 May (2007).
Serrano-Rodríguez, J. M., Cárceles-García, C., Cárceles-Rodríguez, C.M., Gabarda, M.L., Serrano-Caballero, J. M., & Fernández-Varón, E. (2017). Susceptibility and PK/PD relationships of Staphylococcus aureus strains from ovine and caprine with clinical mastitis against five veterinary fluoroquinolones. Vet Rec., 180(15), 376. doi: 10.1136/vr.103964.
Smith, L. T., & Lewin, C. S. (1988). Chemistry and mechanisms of action of the quinolone antibacterials. In “Quinolones”, Ed. Andriole V., Acaem. Press, 23–82.
Stetsko, T. I. (2005). Rezystentnist do ftorkhinoloniv: pokhodzhennia, evoliutsiia, klinichne znachennia ta shliakhy podolannia. Biolohiia tvaryn, 7(1–2), 51–63 (in Ukrainian).
Stetsko, T. I. (2008). Zasady efektyvnoi antybiotyko-terapii u veterynarnii medytsyni. Veterynarna biotekhnolohiia, 13(1), 194–200 (in Ukrainian).
WHO (2011). Critically Important Antimicrobials for human medicine publication. http://www.who.int/ foodsafety/publications/antimicrobials-third/en.
Yakovlev, S. V. (1999). Mesto ftorhinolonov v lechenii bakterialnyih infektsiy. Antibiotiki i himioterapiya, 44(12), 27–30 (in Russian).
Yakovlev, V. P. (1993). Farmakokinetika ftorhinolonov. Antibiotiki i himioterapiya, 38(6), 66–78 (in Russian).
Yoshimura, H., Takagi, M., Ishimura, M., & Endoh, Y. S. (2002). Comparative in vitro activity of 16 antimicrobial agents against Actinobacillus pleuropneumoniae. Vet Res Commun, 26(1), 11–19. doi: 10.1023/a:1013397419995.
Zhao, S., Maurer, J. J., Hubert, S., DeVillena, J. F., McDermott, P. F., Meng, J., Ayers, S., English, L., & White, D. G. (2005). Antimicrobial susceptibility and molecular characterization of avian pathogenic Escherichia coli isolates. Vet. Microbiol., 107, 215–224. doi: 10.1016/j.vetmic.2005.01.021.
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