Investigation of the antimicrobial activity of danofoloxacin against bacteria pathogens in goats


  • T. I. Stetsko State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine https://orcid.org/0000-0002-9240-2191
  • Ya. M. Liubenko State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine https://orcid.org/0000-0001-9924-9014
  • V. N. Padovskyi State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
  • L. L. Ostrovska State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
  • O. Yo. Kalinina State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine https://orcid.org/0000-0002-5504-1825
  • O. Z. Balyan State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
Keywords: fluoroquinolones, danofloxacin, goats, respiratory and intestinal infections, bacteria, Streptococcus pneu-moniae, Staphylococcus aureus, Escherichia coli, sensitivity of microorganisms, minimum inhibitory con-centration.

Abstract

Fluoroquinolones are critical antimicrobials for both human and veterinary medicine. Due to their unique mechanism of antimicrobial action and good pharmacokinetic properties, they are often the first choice drugs in the treatment of bacterial infections in animals. The purpose of the investigation was to study the antimicrobial activity of a third-generation fluoroquinolone antibiotic of danofloxacin against bacteria, pathogens of respiratory and intestinal infection in goats. The samples of the nasal outflows (respiratory infection) and fecal masses (intestinal infection) were collected from clinically ill goats for microbiological studies. The sensitivity test of the microflora of the biomaterial, carried out by the disco-diffusion method, showed that the microorganisms of all the samples were sensitive to danofloxacin. Bacteria Streptococcus pneumonia (n = 10), Staphylococcus aureus (n = 4) and Escherichia coli (n = 2) were isolated and identified from nasal exudate samples (n = 10). Pathogenic strains of Escherichia coli were isolated from all faecal samples (n = 12). The degree of bacteriostatic activity of danofloxacin was determined by establishing its minimum inhibitory concentration (MIC) for bacterial isolates by sequential dilutions in a liquid nutrient medium. The average MIC of danofloxacin for Streptococcus pneumoniae isolates was 0.26 ± 0.13 μg/ml and for Staphylococcus aureus isolates – 0.25 ± 0.075 μg/ml. For Escherichia coli strains isolated from faeces of goats suffering from coli infection, the average MIC of danofloxacin was 0.38 ± 0.12 μg/ml (range 0.2 to 0.8 μg/ml). Antimicrobial sensitivity testing have shown a high level of bacteriostatic activity of danofloxacin against bacteria, pathogens of respiratory and intestinal infections in goats. This may be the argument for the use of danofloxacin-based chemotherapeutic agents in the treatment of bacterial infections in goats, especially for the empirical approach to therapy.

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Published
2020-05-07
How to Cite
Stetsko, T., Liubenko, Y., Padovskyi, V., Ostrovska, L., Kalinina, O., & Balyan, O. (2020). Investigation of the antimicrobial activity of danofoloxacin against bacteria pathogens in goats. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 22(97), 74-78. https://doi.org/10.32718/nvlvet9713