Physiological state of the broiler chickens organism on the background of combined stress and additive of humic acids and probiotic

Keywords: broiler chickens, vaccination, cold stress, adaptation, humic acids, probiotic


The cultivation of broiler chickens is cost-effective given the high reproductive quality and growth rate of young growth at an early age. However, the management of the industry involves a number of technological operations that cause excessive stress on the adaptive systems, decrease the immunobiological reactivity of the bird organism, the development of stress and cause a decline in egg and meat productivity. Under these conditions, it is necessary to strengthen and stimulate the resistance of the body of the bird, to determine ways to eliminate the development of stressful states by providing full feeding. Therefore, the purpose of the study was to determine the morpho-functional status of the body of broiler chickens against the background of combined stress when included in the diet of humic acids “Reasil Humic Vet”, “Reasil Humic Health” and probiotics “Laktin”. For the 13th day of life, all livestock in a clinically healthy poultry were subjected to combined stress – revaccination (intranasal against Newcastle disease) + cold stress (for 60 min by conditioning the room and reducing the vivarium temperature by 5 °C). The study material was sampled 3 days after exposure to stress (corresponding to the anxiety stage), at 13, 20, and 26 days after exposure to stress (which accounted for the various stages of resistance stage development). It is established that the morpho-functional status of the body of broiler chickens on the background of combined stress is characterized at the stage of anxiety by reducing the concentration of hemoglobin and hematocrit by an average of 16.6 %, increasing the number of eosinophils and pseudo-eosinophils; at various stages of development of the stage of resistance – decrease in the number of red blood cells and hemoglobin concentration by 3.4–6.5 % against the background of increase of hematocritic value by 37.0 %, increase in the number of leukocytes by 17.2 % with a slight stabilization of respiratory and protective function of blood due to 26 days after stress. Influencing the formation of adaptive reactions of the body of broiler chickens of the experimental groups in the conditions of development of adaptation syndrome positive influence of the use of new biostimulants of natural origin “Reasil Humic Vet”, “Laktin” and “Reasil Humic Health” is shown, which is shown by the increase of hemoglobin concentration on average 13.0 % (P < 0.05), leukocyte count by 25.4 % (P < 0.05) due to lymphocytes 1.1 times (P < 0.05).


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Osti, R., BhattaraiII, D., & Zhou, D. (2017). Climatic Variation: Effects on Stress Levels, Feed Intake, and Bodyweight of Broilers. Brazilian Journal of Poultry Science, 19(3), 345–350. doi: 10.1590/1806-9061-2017-0494.

Chen, N. N., Liu, B., Xiong, P. W., Guo, Y., He, J. N., Hou, C. C., Ma, L. X., & Yu, D. Y. (2018). Safety evaluation of zinc methionine in laying hens: Effects on laying performance, clinical blood parameters, organ development, and histopathology. Poultry Science, 97(4), 1120–1126. doi: 10.3382/ps/pex400.

Fisinin, V. I., & Kavtarashvili, A. Sh. (2015). Heat stress in poultry: methods and techniques for prevention and alleviation (review). Agricultural BIology, 50(4), 431–443. doi: 10.15389/agrobiology.2015.4.431.

Stojanovskij, V. G., Krogh, A. O. & Kolomіiеts, І. A. (2018). Adaptacіja stanu nespecifіchnoї rezistentnostі organіzmu kachok v umovah stresu pri vkljuchennі v racіon probіotichnih dobavok. Naukovyi visnyk LNUVMB imeni S.Z. Gzhytskoho, 20(87), 32–38. doi: 10.15421/nvlvet8706 (in Ukrainian).

Pavlova, I. (2015). Effect of probiotics on doxycycline disposition in gastro-intestinal tract of poultry. Bulgarian Journal of Veterinary Medicine, 18(3), 248–257. doi: 10.15547/bjvm.908.

Stojanovskij, V. G., Kolomіес, І. A., Garmata, L. S., & Kamracka, O. І. (2018). Zmіni morfofunkcіonalnogo stanu organіv endokrinnoі ta іmunnoі sistem perepelіv promislovogo viroshhuvannja za dіі stresu. Fіzіologіchnij zhurnal, 64(1), 25–33. doi: 10.15407/fz64.01.025 (in Ukrainian).

Olubodun, J., Zulkifli, I., Hair-Bejo, M., Kasim, A., & Soleimani, A. F. (2015). Physiological response of glutamine and glutamic acid supplemented broiler chickens to heat stress. European Poultry Science, 79–82. doi: 10.1399/eps.2015.87.

Gutyj, B, Leskiv, K., Shcherbatyy, A., Pritsak, V., Fedorovych, V., Fedorovych, O., Rusyn, V., & Kolomiiets, I. (2017). The influence of Metisevit on biochemical and morphological indicators of blood of piglets under nitrate loading. Regul. Mech. Biosyst. 8(3), 427–432. doi: 10.15421/021766.

Kapustian, A., Cherno, N., Stankevich, G., Kolomiіets, I., Matsjuk, O., Musiy, L. & Slyvka, I. (2018). Determination of the enzyme destruction rational mode of biomass autolysate of lactic acid bacteria. Eastern-European Journal of Enterprise Technologies, 1/11 (91), 63–68. doi: 10.15587/1729-4061.2018.120877.

Vlizlo, V. V., Fedoruk, R. S. & Ratіch, I. B. (2012). Laboratornі metodi doslіdzhen u bіologіі, tvarinnictvі ta veterinarnіj medicinі: dovіdnik (in Ukrainian).

Mohammed, A. A., Jacobs, J. A., Murugesan, G. R., & Cheng, H. W. (2018). Effect of dietary synbiotic supplement on behavioral patterns and growth performance of broiler chickens reared under heat stress. Poultry Science, 97(4), 1101–1108. doi: 10.3382/ps/pex421.

Nguyen, P. H., Greene, E., Donoghue, A., Huff, G., Clark, F. D., & Dridi, S. (2016). A new insight into cold stress in poultry production. Adv. Food. Technol. Nutr. Sci. Open. J., 2(1), 1–2. doi: 10.17140/AFTNSOJ-2-124.

Hangalapura, B. N., Nieuwland, M. G. B., Buyse, J., Kemp, B., & Parmentier, H. K. (2004). Effect of duration of cold stress on plasma adrenal and thyroid hormone levels and immune responses in chicken lines divergently selected for antibody responses. Poult. Sci., 83(10), 1644–1649. doi: 10.1093/ps/83.10.1644.

Varkholiak, I., & Gutyj, B. (2020). The influence of the preparation “Bendamin” on the morphological and biochemical indices of blood of rats in experimental modeling of heart failure. Ukrainian Journal of Veterinary and Agricultural Sciences, 3(1), 38–41. doi: 10.32718/ujvas3-1.07.

Tsiouris, V., Georgopoulou, I., Batzios, C., Pappaioannou, N., Ducatelle R., & Fortomaris P. (2015). The effect of cold stress on the pathogenesis of necrotic enteritis in broiler chicks, Avian Pathology, 44(6), 430–435, doi: 10.1080/03079457.2015.1083094.

Qureshi, S., Khan, H. M., Mir, M. S., Raja, T. A., Khan, A. A., Ali, H., & Adil, S. (2018). Effect of Cold Stress and Various Suitable Remedies on Performance of Broiler Chicken. J. World Poult. Res., 8(3), 66–73.

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Stoyanovskyy, V., Shevchuk, M., & Kolomiiets, I. (2020). Physiological state of the broiler chickens organism on the background of combined stress and additive of humic acids and probiotic. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 22(97), 157-161.