Influence of probiotics “Immunobacterin-D” on biocenoses and development of the gastrointestinal tract of calves

Keywords: rumen, microflora, chewing, digestion, “Immunobacterin-D”


The paper presents the results of an experiment on the use of enzyme-probiotic supplement “Immunobacterin-D” for calves in the first two months of life. Probiotics are used in animal husbandry for the prevention and treatment of infectious diseases, increase immunity, correction of the microflora after antibiotic therapy, prevention of stress, increase growth. The study was conducted in a private farm in the Lyubar district of Zhytomyr region, which kept cattle of different ages. In a randomized controlled trial, two experimental groups of six animals were formed in each on the principle of analogues. One group was a control. Calves of the experimental group from the second day after birth were fed together with colostrum “Immunobacterin-D” at the rate of 3 grams per animal. For the first five days, the enzyme-probiotic supplement was given to the calves once a day during the morning feeding, and in subsequent studies, once every two days for a month. The results of the studies show that no case of diarrhea was registered among calves receiving the enzyme-probiotic supplement “Immunobacterin-D”. Among the animals of the control group, 66.7 % had digestive disorders – dyspepsia. Subsequently, such animals had a lower growth rate. Enzyme-probiotic supplement “Immunobacterin-D” also contains Bacillus Subthilis, Bacillus Licheniformis and enzymes and protease and lipase – it provides a polyvector effect in the digestive tract in general and scarring in particular. It was found that on the fourteenth day of feeding probiotic-containing drug “Immunobacterin-D” to calves of the experimental group, the number of bacteria probably increased by 83.5 %; ciliates – by 65.0 %; endodynomorphs – by 24.3 %. After one month of the experiment, the number of bacteria in the experimental group probably increased by 94.3 %; ciliates – by 40.5 %; endodynomorphs – by 26.7 %, compared with the control (P < 0.001). The obtained data of registration of the first chewing gum in calves prove that the components of the enzyme-probiotic supplement “Immunobacterin-D” accelerate the population of microflora and the development of scar digestion in calves 2.5 times compared to calves in the control group. Prospects for further research are to study the effect of enzyme-probiotic supplement “Immunobacterin-D” on the indicators of scar fermentation of calves.


Download data is not yet available.


Aikman, P. C., Henning, P. H., Humphries, D. J., & Horn, C. H. (2010). Rumen pH and fermentation characteristics in dairy cows supplemented with Megasphaera elsdenii NCIMB 41125 in early lactation. J. Dairy Sci., 94(6), 2840–2849. doi: 10.3168/jds.2010-3783.

Aschenbach, J. R., Zebeli, Q., Patra, A. K., Greco, G., Amasheh, S., & Penner, G. B. (2019) Symposium re-view: The importance of the ruminal epithelial barrier for a healthy and productive cow. J Dairy Sci., 102(2), 1866–1882. doi: 10.3168/jds.2018-15243.

Basso, F. C., Adesogan, A. T., Lara E. C., Rabelo, C. H. S., Berchielli, T. T., Teixeira, I. A. M. A., Siqueira, G. R., & Reis, R. A. (2014). Effects of feeding corn silage in-oculated with microbial additives on the ruminal fer-mentation, microbial protein yield, and growth per-formance of lambs. J Anim Sci., 92(12), 5640–5650. doi: 10.2527/jas.2014-8258.

Diao, Q., Zhang, R., & Tu, Y. (2017). Current research progresses on calf rearing and nutrition in China. J. Integr., 16(2), 2805–2814. doi: 10.1016/S2095-3119(17)61767-2.

Fan, P., Li, L., Rezaei, A., Eslamfam, S., Che, D., & Ma, X. (2015). Metabolites of dietary protein and peptides by intestinal microbes and their impacts on the gut. Curr Protein Pept Sci., 16(7), 646–654. doi: 10.2174/1389203716666150630133657.

Gerritsen, J., Smidt, H., Rijks, G. T., & Vos, W. M. (2011) Intestinal microbiota in human health and disease: the impact of probiotics. Genes Nutrition, 6(3), 209–240. doi: 10.1007/s12263-011-0229-7.

Izuddin, W. I., Humam, A. M., Loh, T. C., Foo, H. L., & Samsudin, A. A. (2020). Dietary Postbiotic Lactoba-cillus plantarum Improves Serum and Ruminal Anti-oxidant Activity and Upregulates Hepatic Antioxidant Enzymes and Ruminal Barrier Function in Post-Weaning Lambs. Antioxidants (Basel, Switzerland), 9(3), 250. doi: 10.3390/antiox9030250.

Izuddin, W. I., Loh, T. C., Samsudin, A. A., Foo, H. L., Humam, A. M., & Shazali, N. (2019). Effects of post-biotic supplementation on growth performance, rumi-nal fermentation and microbial profile, blood metabo-lite and GHR, IGF-1 and MCT-1 gene expression in post-weaning lambs. BMC Vet Res., 15(1), 315. doi: 10.1186/s12917-019-2064-9.

Jia, P., Cui, K., Ma, T., Wan, F., Wang, W., Yang, D., Wang, Y., Guo, B., Zhao, L., & Diao, Q. (2018). Influ-ence of dietary supplementation with Bacillus licheni-formis and Saccharomyces cerevisiae as alternatives to monensin on growth performance, antioxidant, immunity, ruminal fermentation and microbial diver-sity of fattening lambs. Scientific reports, 8(1), 16712. doi: 10.1038/s41598-018-35081-4.

Kong, L., Yang, C., Dong, L., Diao, Q., Si, B., Ma, J., & Tu, Y. (2019). Rumen Fermentation Characteristics in Pre- and Post-Weaning Calves upon Feeding with Mulberry Leaf Flavonoids and Candida tropicalis Individually or in Combination as a Supplement. Animals: an open access journal from MDPI, 9(11), 990. doi: 10.3390/ani9110990.

Larsen, N., Thorsen, L., Kpikpi, E. N., Stuer-Lauridsen, B., Cantor, M. D., Nielsen, B., Brockmann, E., Derkx, P. M. F., & Jespersen, L. (2014). Characterization of Ba-cillus spp. strains for use as probiotic additives in pig feed. Appl Microbiol Biotechnol., 98(3), 1105–1118. doi: 10.1007/s00253-013-5343-6.

Liu, X., Zhao, W., Yu, D., Cheng, J. G., Luo, Y., Wang, Y., Yang, Z. X., Yao, X. P., Wu, S. S., Wang, W. Y., Yang, W., Li, D. Q., & Wu, Y. M. (2019). Effects of compound probiotics on the weight, immunity performance and fecal microbiota of forest musk deer. Scientific reports, 9(1), 19146. doi: 10.1038/s41598-019-55731-5.

Murphy, S. C., & Boor, K. J. (2000). Trouble-shooting sources and causes of high bacteria counts in raw milk. Dairy, Food and Environmental Sanitation, 8, 606–611.

Rautava, S., Luoto, R., Salminen, S., & Isolauri, E. (2012). Microbial contact during pregnancy, intestinal colonization and human disease. Nat Rev Gastroen-terol Hepatol, 9(10), 565–576. doi: 10.1038/nrgastro.2012.144.

Sahadeva, R., Leong, S., & Chua, K. (2011). Survival of commercial probiotic strains to pH and bile. International Food Research Journal, 18, 1515–1522.

Silva, L. D. D., Pereira, O. G., Silva, T. C. D., Valadares Filho, S. C., & Ribeiro, K. G. (2016). Effects of silage crop and dietary crude protein levels on digestibility ruminal fermentation, nitrogen use efficiency, and performance of finishing beef cattle. Anim Feed Sci Technol, 220, 22–33. doi: 10.1016/j.anifeedsci. 2016.07.008.

Soares, M. S., Oliveira, P. S., Debom, G. N., DaSilveira, M. B., Polachini, C. R., Baldissarelli, J., et al. (2017). Chronic administration of methionine and/or methionine sulfoxide alters oxidative stress parameters and ALA-D activity in liver and kidney of young rats. Amino Acids, 49(1), 129–138. doi: 10.1007/s00726-016-2340-y.

Stover, P. J., Durga, J., & Field, M. S. (2017). Folate nutrition and blood–brain barrier dysfunction. Curr Opin Biotechnol. 44, 146–152. doi: 10.1016/j.copbio.2017.01.006.

Sun, P., Wang, J. Q., & Zhang, H. T. (2010). Effects of Bacillus subtilis natto on performance and immune function of preweaning calves. J Dairy Sci., 93(12), 5851–5855. doi: 10.3168/jds.2010-3263.

Tan, J., McKenzie, C., Potamitis, M., Thorburn, A. N., Mackay, C. R., & Macia, L. (2014). The role of short-chain fatty acids in health and disease. Adv Immu-nol., 121, 91–119. doi: 10.1016/B978-0-12-800100-4.00003-9.

Abstract views: 10
PDF Downloads: 10
How to Cite
Rybachuk, Z., Shkromada, O., Predko, A., & Dudchenko, Y. (2020). Influence of probiotics “Immunobacterin-D” on biocenoses and development of the gastrointestinal tract of calves. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 22(98), 22-27.