Determination of the activity of fungicides against pathogens of dermatomycoses in domestic animals


  • I. M. Kushnir State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine https://orcid.org/0000-0001-6117-9735
  • V. I. Kushnir State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine https://orcid.org/0000-0002-9374-2454
  • B. V. Gutyj Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, Ukraine https://orcid.org/0000-0002-5971-8776
  • I. S. Semen State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine https://orcid.org/0000-0003-1162-3847
  • S. D. Murska State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine https://orcid.org/0000-0001-8394-1858
  • G. V. Kolodiy State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
  • U. Z. Berbeka State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
Keywords: chlorhexidine digluconate, ketonazole, microscopic fungi, epidermatophytes, mycoses

Abstract

The aim of the study was to identify and identify the causative agents of dermatomycosis in dogs and cats and to determine their sensitivity to solutions of ketonazole and chlorhexidine digluconate, which are part of the drug Skinhard. This is a spray for external use, manufactured by PJSC “Halychpharm”. For laboratory diagnosis of dermatomycoses, samples from the affected parts of the skin and fur of domestic animals were used. To isolate a pure culture of fungi were sown on selective media: wort agar, agar Saburo, Chapek. The optimal cultivation regime for pathogenic fungi was 20–25 °С. Isolated fungal cultures were identified by the appearance and shape of colonies, their consistency, color, ability to grow at 37 °C, microscopic structure, in particular - the nature of mycelial branching and the presence of septa, location of conidiophores, spores and other signs. A microbiological examination of the affected areas of the skin of dogs and cats was performed to detect microscopic fungi. The following fungi were isolated from dogs affected by mycoses: Candida spp., Aspergillus niger, Epidermophyton spp., Microsporum spp., Mucor spp., Trichophyton spp., Mallasseria spp., Sporotrich spp, Candida albicans, and from cats: Microspor Candida spp., Spototrich spp., Rhizorus spp., Fusarium spp. Trichophyton spp. Isolated microscopic fungi of the genus Malassezia spp. belong to superficial mycoses (keratomycoses) and affect the superficial layers of skin and hair. Epidermatophytes: Trichophyton spp., Epidermophyton spp., Microsporum spp. affect the epidermis, skin and coat. In addition, opportunistic fungi of the genus Aspergillus spp, Mucor spp., Rhizorus spp. and Fusarium spp. Chlorhexidine digluconate was found to be highly active against dermatophytes (Trichophyton spp., Microsporum spp.), Less active against fungi of the genus Candida and less active against Aspergillus spp, Mucor spp., Rhizorus spp, Fusarium spp. Ketonazole is highly active against dermatophytes (Trichophyton spp., Epidermophyton spp., Microsporum spp.), Yeast (Malassezia spp., Candida spp.,) And opportunistic fungi (Aspergillus spp., Mucor spp., Rhizorus spp.).

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Abstract views: 159
PDF Downloads: 107
Published
2020-10-28
How to Cite
Kushnir, I., Kushnir, V., Gutyj, B., Semen, I., Murska, S., Kolodiy, G., & Berbeka, U. (2020). Determination of the activity of fungicides against pathogens of dermatomycoses in domestic animals. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 22(99), 20-23. https://doi.org/10.32718/nvlvet9903

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