Influence of protein-mineral feed additive from marine aquatic organisms on growth intensity and nonspecific resistance of broiler chickens under different microclimate conditions

Keywords: broiler chickens, aquatic organisms, mussels, red algae, haemoglobin, erythrocytes, bactericidal lysozyme activity, microclimate parameters


The conducted research was aimed at determination of the impact produced by feeding protein and mineral feed additive produced out of the primary processing aquatic organisms wastes: sea mussels, red algae as well as of the sea water upon the productivity and non-specific resistance of broiler chickens raised in conditions of the normative and non-normative characteristics of the broiler house microclimate. The feed additive was applied to 20–42 day broiler chickens of “Ross 308” cross. The studied broilers were clinically healthy. Throughout the entire experiment, a series of the sanitary and hygienic microclimate parameters were determined, such as temperature, humidity, rate of changes as well as bacterial contamination of air, content of ammonia and carbon dioxide in air, and illumination of the broiler house. The house temperature was measured every day with the aid of a common spirit-in glass thermometer. Air humidity was established with the aid of an August psychrometer, air draft speed, harmful gas concentrations and illumination indicators were measured in compliance with the generally accepted methods. Bacterial contamination was determined with the use of the method of microorganism precipitation on a solid breeding ground placed in Petri dishes followed by a count of the bacterial colonies per 1 m3. The blood analysis included determination of haemoglobin, erythrocytes and leukocytes. In the blood serum, the lysozyme activity (LABS) and bactericidal activity (BABS) were determined. It was established that enriching the basic ration with the protein and mineral additive in quantity of 7 % in addition to the feed mass under conditions of the normative microclimate produced a positive effect on the growth intensity, livability and non-specific resistance indices of the broiler chickens. Thus, the live weight of broilers was reliably greater by 4.7 % and the livability equalled 100 %. The haemoglobin content was reliably greater by 7.6 %, erythrocytes – by 11.5 %, BABS – by 34.5 % and LABS – by 35.9 % as compared with the control group of broiler chickens. At the same time, when the studied feed additive was fed to broiler chickens kept in the microclimate conditions that did not meet the normative requirements, the reliable difference to the control indices was not established. Hence, the research results have proved that application of the protein and mineral feed additive is effective under the optimal microclimate conditions. High figures of livability and growth intensity of broiler chickens are based on a high resistance which is being formed provided the optimal microclimate and application of feed additives have been provided.


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Beski S. S. M., Swick R. A., & Iji, P. A. (2015). Specialized protein products in broiler chicken nutrition: A review. Animal Nutrition, 1(2), 47–53. doi: 10.1016/j.aninu.2015.05.005.

Bogatko, N., Bogatko, L., Salata, V., Semaniuk, V., Ser-dioucov, J., & Schyrevuch, G. (2017). Veterinary-sanitary control of safety and quality of meat prod-ucts. Scientific Messenger of LNU of Veterinary Med-icine and Biotechnologies. Series: Veterinary Sciences, 19(73), 7–10. doi: 10.15421/nvlvet7302.

Bueno, J. P. R., Nascimento, M. R. B. M., Martins, J. M. S., Marchini, C. F. P., Gotardo, L. R. M., Sousa, G. M. R., Mundim, A. V., Guimarães, E. C., & Rinaldi, F. P. (2017). Effect of age and cyclical heat stress on the serum biochemical profile of broiler chickens Influência da idade e do estresse cíclico de calor no perfil bioquímico sérico em frangos de corte. Semina: Ciências Agrárias, Londrina, 38(3), 1383–1392. doi: 10.32819/2020.81009.

Buğdaycı, K. E., Gümüş, H., Oğuz, M. N., Karakaş Oğuz, F., & Gülle, İ. (2019). Effects of Mediterranean Mus-sel Shell (Mytilus galloprovincialis) on Performance and Egg Quality in Laying Quails. Acta Vet Eurasia, 45, 22–29. doi: 10.26650/actavet.2019.18010.

de Jesus Raposo, M. F., de Morais, A. M., & de Morais, R. M. (2015). Marine polysaccharides from algae with potential biomedical applications. Marine Drugs, 13, 2967–3028. doi: 10.3390/md13052967.

Demchuk, M. V., Chorny`j, M. V., Zaxarenko, M. O., & Vy`sokos, M. P. (2006). Gigiyena tvary`n: pidruchny`k. Xarkiv: Espada (in Ukrainian).

Gómez-Ordóñez, E., Jiménez-Escrig, A., & Rupérez, P. (2012). Effect of the red seaweed Mastocarpus stella-tus intake on lipid metabolism and antioxidant status in healthy Wistar rats. Food Chemistry. 135, 806–811. doi: 10.1016/j.foodchem.2012.04.138.

Hrabčáková, P., Voslářová, E., Bedáňová, I., Pištěková, V., Chloupek, J., & Večerek, V. (2014). Haematological and biochemical parameters during the laying period in common pheasant hens housed in enhanced cages. ScientificWorldJournal, 2014, 364602. doi: 10.1155/2014/364602.

Ikusika, O. O., Mpendulo, C. T., Zindove, T. J., & Okoh, A. I. (2019). Fossil shell flour in livestock production: A Review. Animals (Basel), 9(3), 70. doi: 10.3390/ani9030070.

Jonsson, L., & Elwinger, K. (2009). Mussel meal as a re-placement for fish meal in feeds for organic poultry – a pilot short term study. Acta Agriculturae Scandina-vica – Section A. Animal Science, 59, 22–27. doi: 10.1080/09064700902730158.

Kovbasenko, V. M., & Dronova, N. I. (2008). Sposib oderzhannya kormovoyi dobavky` z mors`ky`x gidrobiontiv dlya pty`ci: pat. 34634 Ukrayina. u4200808275; zayavl. 19.06.2008; opubl. 11.08.2008; Byul. 15, 4 (in Ukrainian).

Kovbasenko V. M., & Karajvan N. I. (2009). Sposib oderzhannya kormovoyi dobavky` z mors`ky`x gidrobiontiv dlya pty`ci: pat. 42687 Ukrayina. u 4200903402; zayavl. 09.04.2009; opubl. 10.07.2009; Byul. 13, 4 (in Ukrainian).

Kukhtyn, M., Salata, V., Berhilevych, O., Malimon, Z., Tsvihun, A., Gutyj, B., & Horiuk, Y. (2020). Evalua-tion of storage methods of beef by microbiological and chemical indicators. Potravinarstvo Slovak Journal of Food Sciences, 14, 602–611. doi: 10.5219/1381.

McLaughlan, C., Rose, P., David, C., & Aldridge, D. C. (2014). Making the Best of a Pest: The potential for using invasive zebra mussel (Dreissena polymorpha) biomass as a supplement to commercial chicken feed. Environmental Management, 54, 1102–1109. doi: 10.1007/s00267-014-0335-6.

Morris, J. P., Backeljau, T., & Chapelle, G. (2019). Shells from aquaculture: a valuable biomaterial, not a nui-sance waste product. Reviews in Aquaculture, 11, 42–57. doi: 10.1111/raq.12225.

Oso, A. O., Idowu, A. A., & Niameh, O. T. (2011). Growth response, nutrient and mineral retention, bone mineralisation and walking ability of broiler chickens fed with dietary inclusion of various unconventional mineral sources. Journal of Animal Physiology and Animal Nutrition, 95(4), 461–467. doi: 10.1111/j.1439-0396.2010.01073.x.

Souza, B. W. S., Cerqueira, M. A., Bourbon, A. I., Pinhei-ro, A. C., Martins, J. T., Teixeira, J. A., Coimbra, M., & Vicente A. (2012). Chemical characterization and an-tioxidant activity of sulfated polysaccharide from the red seaweed Gracilaria birdiae. Food Hydrocolloids, 27, 287–292. doi: 10.1016/j.foodhyd.2011.10.005.

Świątkiewicz, S., & Arczewska-Wlosek, A. (2012). Bone quality characteristics and performance in broiler chickens fed diets supplemented with organic acids. Czech Journal of Animal Science, 57(4), 193–205. doi: 10.17221/6004-cjas.

Szabó, A., Mézes, M., Horn, P., Sütő, Z., Bázár, G. Y., & Romvári, R. (2005). Developmental dynamics of some blood biochemical parameters in the growing turkey (Meleagris gallopavo). Acta Veterinaria Hun-garica, 53(4), 397–409. doi: 10.1556/AVet.53.2005.4.1.

Ventura, M. V. A., & da Silva, R. M. (2019). Bone problems caused by the deficiency of calcium and phosphorus in the feeding of broilers. Biomedical Journal of Scientific & Technical Research, 16(4), 12223–12226. doi: 10.26717/BJSTR.2019.16.002886.

Vlizlo, V. V., Fedoruk, R. S., & Raty`ch, I. B. (2012). Laboratorni metody` doslidzhennya u biologiyi, tvary`nny`cztvi ta vetery`narnij medy`cy`ni: dovidny`k. L`viv: Spolom (in Ukrainian).

Xing, R., Yang, H., Wang, X., Yu, H., Liu, S., & Li, P. (2020). Effects of calcium source and calcium level on growth performance, immune organ indexes, se-rum components, intestinal microbiota, and intestinal morphology of broiler chickens. The Journal of Ap-plied Poultry Research, 29(1), 106–120. doi: 10.3382/japr/pfz033.

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How to Cite
Dankevych, N., & Salata, V. (2020). Influence of protein-mineral feed additive from marine aquatic organisms on growth intensity and nonspecific resistance of broiler chickens under different microclimate conditions. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 22(99), 155-160.

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