The content of total protein and protein fractions in cows during pregnancy and their diagnostic value


Keywords: pregnancy, protein, albumins, globulins, uterine subinvolution

Abstract

The aim of the article was to study the content of total protein and protein fractions in the blood of cows during pregnancy and determine their diagnostic value. The studies were performed as follows. Within 7 days after insemination on the principle of analogues selected a group of 100 cows. Pregnancy was diagnosed by rectal examination of cows 60 days after insemination. In experimental animals, starting from the 25th to the 29th day after insemination, blood samples for morphological and biochemical studies were taken monthly before delivery. The total protein content in the serum was determined by the biuret reaction, protein fractions – nephelometric method. Depending on the course of labor and the postpartum period, cows were divided into two groups. The first group included 30 animals with a physiological course of the postpartum period. The second group was formed of 30 cows with subinvolution of the uterus. According to this goal, the dynamics of total protein, albumin, alpha-, beta- and gammaglobulins in the blood of cows with physiological course of pregnancy and prone to the development of subinvolution of the uterus from the first to 9 months of pregnancy. It was found that in clinically healthy cows the onset of pregnancy is characterized by a high (0.89) albumin-globulin ratio, which in the fourth month of pregnancy decreases to 0.78, in the fifth or sixth months increases to 0.85, followed by a decrease to 0.78 before childbirth. In cows prone to uterine subinvolution, a significant difference in albumin content was found in the first month of pregnancy (47.07 ± 1.21 vs. 41.02 ± 1.26 %). In the third to fifth months of pregnancy, there was an increase in the proportion of albumin in the protein to 44.62 ± 0.88 % and a new decrease in the seventh to ninth months to 41.96 ± 0.3 %. In the first month of pregnancy, the albumin-globulin ratio was 0.7, with a tendency to increase in the fifth to sixth months of pregnancy to 0.8, and decrease to 0.72 before calving. Analyzing the dynamics of alpha-globulins in clinically healthy cows, we noted two peaks of decrease in their percentage - in the third month of pregnancy (from 16.02 ± 0.08 to 15.14 ± 0.06 %) and in the fifth month , 96 ± 0.48 %) and a consistent increase in the seventh to ninth months of pregnancy (up to 17.22 ± 0.13 %). And in cows prone to the development of uterine subinvolution, with a slightly higher percentage at the beginning of pregnancy (16.02 ± 0.08 to 17.08 ± 0.05 %), we did not notice significant changes in the percentage of this globulin, except for a significant increase in seventh to ninth month of pregnancy (up to 18.01 ± 0.24 – 19.01 ± 0.32 %). It is proposed to use the albumin-globulin ratio at 6, 7, 8, 9 months of gestation as a prognostic factor in the detection of cows prone to uterine subinvolution.

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References

Borshch, O. O., Gutyj, B. V., Sobolev, O. I., Borshch, O. V., Ruban, S. Yu., Bilkevich, V. V., Dutka, V. R., Chernenko, O. M., Zhelavskyi, M. M., & Nahirniak, T. (2020). Adaptation strategy of different cow genotypes to the voluntary milking system. Ukrainian Journal of Ecology, 10(1), 145–150. doi: 10.15421/2020_23.

Grymak, Y., Skoromna, O., Stadnytska, O., Sobolev, O., Gutyj, B., Shalovylo, S., Hachak, Y., Grabovska, O., Bushueva, I., Denys, G., Hudyma, V., Pakholkiv, N., Jarochovich, I., Nahirniak, T., Pavliv, O., Farionik, Т., & Bratyuk, V. (2020). Influence of “Thireomagnile” and “Thyrioton” preparations on the antioxidant sta-tus of pregnant cows. Ukrainian Journal of Ecology, 10(1), 122–126. doi: 10.15421/2020_19.

Kejik, C., & Maskova, A. (1989). Termovizni mereni povrchovych teplot vemen vprubehu strojnio dojeni. Zemed techn, 4, 305–307.

Klosova, X. G., Bushueva, I. V., Parchenko, V. V., Shcherbyna, R. O., Samura, T. О., Gubenko, I. Ya., Gutyj, B. V., & Khariv, I. I. (2019). Trifuzol Supposi-tories Usage Results On The Course Of Endometrial Inflammatory Processes In Cows. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 10(1), 1215−1223. URL: https://www.rjpbcs.com/pdf/ 2019_10(1)/[157].pdf.

Kondrahin, I. P. (1998). Polimorbidnost' vnutrennej patologii. Vіsnik Bіlocerkіvs'kogo derzhavnogo agrar-nogo unіversitetu, 5(1), 79–83 (in Russian).

Kondrahin, I. P. (2005). Izuchenie sochetannyh vnutren-nih boleznej zhivotnyh prioritetnoe nauchnoe naprav-lenie. Veterinarija, 1, 44–48 (in Russian).

Kondrahin, I. P., Arhipov, A. V., & Lechenko, V. I. (2004). Metody veterinarnoj klinicheskoj laboratornoj diag-nostiki. M.: KolosS (in Russian).

Kucherenko, M. Ie., Babeniuk, Yu. D., & Voibitskyi, V. M. (2001). Suchasni metody biokhimichnykh doslidzhen. K.: Fitosotsiotsentr (in Ukrainian).

Kulyaba, O., Stybel, V., Gutyj, B., Turko, I., Peleno, R., Turko, Ya., Golovach, P., Vishchur, V., Prijma, O., Mazur, I., Dutka, V., Todoriuk, V., Golub, O. Dmytriv, O., Oseredchuk, R. (2019). Effect of experimental fascioliasis on the protein synthesis function of cow liver. Ukrainian Journal of Ecology, 9(4), 612–615. URL: https://www.ujecology.com/abstract/effect-of-experimental-fascioliasis-on-the-protein-synthesis-function-of-cow-liver-44972.html.

Lakin, G. F. (1990). Biometrija. M.: Vyssh. shk. (in Rus-sian).

Lenec, I. A. (1989). Diagnostika nezaraznyh boleznej zhivotnyh s primeneniem vychislitel'noj tehniki. M.: Agropromizdat (in Russian).

Malinowski, E., & Kaczmarowski, M. (2003). Zatrzymanie lozyska u krow. Med. Veter., 59(5), 378–381.

Mazur, N. P., Fedorovych, V. V., Fedorovych, E. I., Fedo-rovych, O. V., Bodnar, P. V., Gutyj, B. V., Kuziv, M. I., Kuziv, N. M., Orikhivskyi, T. V., Grabovska, O. S., Denys, H. H., Stakhiv, N. P., Hudyma, V. Yu., & Pakholkiv, N. I. (2020). Effect of morphological and biochemical blood composition on milk yield in Sim-mental breed cows of different production types. Ukrainian Journal of Ecology, 10(2), 61–67. doi: 10.15421/2020_110.

Temnyi, M. (2005). Polietiolohichna prychyna neplidnosti koriv – realnist sohodennia. Veterynarna medytsyna Ukrainy, 9, 27–28 (in Ukrainian).

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Published
2020-10-28
How to Cite
Stravsky, Y., Boltyk, N., Sachuk, R., Serheyev, V., & Rushchynska, T. (2020). The content of total protein and protein fractions in cows during pregnancy and their diagnostic value. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 22(99), 198-202. https://doi.org/10.32718/nvlvet9930