Study of the parameters of acute toxicity of the drug “Devimectin 1 %” with a single subcutaneous injection in white rats
In the study of the drug for injectable use – “Devimectin 1 %”, together with the confirmation of therapeutic properties, it is necessary to determine the LD50 obtained in the study of acute toxicity. The aim of the work was to study the acute toxicity of “Devimectin 1 %” in white rats by injection. To fulfill this goal on the principle of analogues was formed control and three experimental groups of 4 animals each (n = 4). The drug was administered in doses of 5000.0; 10000.0; 20000.0 mg/kg body weight in absolute weight of the drug once subcutaneously in the withers. The control animals were injected subcutaneously with sterile saline 1.0 cm3. After taking into account the results of the previous experiment in the main experiment, 7 experimental groups were formed, whose rats were injected subcutaneously with “Devimectin 1 %” in doses of 5000.0; 7500.0; 10000.0; 12500.0; 15000.0; 17500.0 and 20000.0 mg/kg body weight, as well as a control group to which animals were injected with sterile saline with a volume of 1.0 cm3. There were 6 animals in each group (n = 6). It was found that for the administration of the drug at a dose of 5000 mg / kg body weight, no animal died, for 10000.0 and 20000.0 mg/kg body weight, respectively, one and 4 animals died. Death occurred for 2–6 days depending on the administered dose. In the main experiment with subcutaneous administration of “Devimectin 1 %” at a dose of 5000.0 mg/kg body weight during the 14-day period of the study, no animal died; for the introduction of the drug at a dose of 7500.0 mg/kg killed one animal; for 10000.0 – 2; for 12500.0 and 15000.0 – 3 rats; for 17500.0 – 5 rats and for the introduction of the drug at a dose of 20000.0 mg/kg body weight, all experimental animals died. The death of laboratory animals occurred for 2–6 days depending on the administered dose. According to the results of studies, it was found that the LD50 of the drug “Devimectin 1 %” under the conditions of its single subcutaneous administration to female rats is 12881.20 ± 1390.54 mg/kg, LD10 – 5978.43 mg/kg, LD16 – 7495.68 mg/kg, LD84 – 18266.73 mg/kg, LD90 – 19783.98 mg/kg, LD100 – 20959.49 mg/kg body weight, respectively. Therefore, the drug “Devimectin 1%” when administered subcutaneously can be classified as toxicity class VI – substances relatively harmless (LD50subcut> 4500,0 mg/kg). Further studies will be the next step in pre-registration trials to examine the subacute toxicity of “Devimectin 1 %”.
European convention for the protection of vertebrate animals used for experimental and other scientific purposes (1986). Council of Europe. Strasbourg. URL: https://rm.coe.int/168007a67b.
GOST 12.1.007-76. SSBT. Vrednye veshhestva. Klassifikacija i obshhie trebovanija bezopasnosti. M.: Izd-vo standartov (in Russian).
Gutyj, B., Grymak, Y., Hunchak, V., Mysak, A., Nazaruk, N., Brezvyn, O., Hariv, I., Shcherbatyy, A., Semeniv, B., Bushueva, I., Parchenko, V., & Kaplaushenko, A. (2018). Preclinical searches of the preparation Thireomagnile. Ukrainian Journal of Ecology, 8(1), 688–695. doi: 10.15421/2018_267.
Gutyj, B., Khariv, I., Binkevych, V., Binkevych, O., Levkivska, N., Levkivskyj, D., & Vavrysevich, Y. (2017). Research on acute and chronic toxity of the experimental drug Аmprolinsyl. Regul. Mech. Biosyst., 8(1), 41–45. doi: 10.15421/021708.
Gutyj, B., Paska, M., Levkivska, N., Pelenyo, R., Nazaruk, N., & Guta, Z. (2016). Study of acute and chronic toxicity of ‘injectable mevesel’ investigational drug. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6(2), 174–180. doi: 10.15421/201649.
Karkyshchenko, N. N., & Hrachev, S. V. (2010). Rukovodstvo po laboratornуm zhyvotnуm y alternatyvnуm modeliam v byomedytsynskykh yssledovanyiakh. M.: Profyl (in Russian).
Kotsiumbas, I. Ya. (2005). Doklinichni doslidzhennia veterynarnykh likarskykh zasobiv. Lviv: Triada plius. (in Ukrainian).
Stattia 26 Zakonu Ukrainy № 5456-VI vid 16.10.2012 r. “Pro zakhyst tvaryn vid zhorstokoho povodzhennia”. URL: https://zakon.rada.gov.ua/laws/show/3447-15#Text (in Ukrainian).
Todoriuk, V. B., Hunchak, V. M., Gutyj, B. V., Gufriy, D. F., Hariv, I. I., Khomyk, R. I., & Vasiv, R. O. (2018). Preclinical research of the experimental preparation “Ferosel T”. Ukrainian Journal of Veterinary and Agri-cultural Sciences, 1(1), 3–9. doi: 10.32718/ujvas1-1.01
Zapadniuk, Y. V. (1983). Laboratornye zhyvotnye. Razvedenye, soderzhanye, yspolzovanye v ek-sperymente. K.: Vyshcha shkola (in Ukrainian).
Abstract views: 15 PDF Downloads: 11