The effect of bendamine on the intensity of lipid peroxidation and the activity of the antioxidant defense system of blood in rats in experimental doxorubicin-induced cardiomyopathy
Experimental studies obtained in recent years indicate the urgency of developing drugs of complex action to prevent the development of cardiovascular disease in dogs. This requires a much deeper study of the pathogenesis of heart failure, including cardiomyopathy. The aim of the study was the effect of bendamine on the intensity of lipid peroxidation and the activity of the antioxidant defense system of blood in rats in experimental doxorubicin-induced cardiomyopathy. To reproduce heart failure in rats, an experimental model was used by intraperitoneal administration of doxorubicin at a dose of 2.5 mg/kg 3 times a week for two weeks. The study was performed on white sexually mature young male Wistar rats weighing 180–200 g, which were kept on the standard diet of the institute vivarium of the State Research Control Institute of Veterinary Drugs and Feed Additives. For the study, three groups of rats of 6 animals in each were formed: control group – intact animals; experimental group R1, in which animals were simulated doxorubicin-induced cardiomyopathy by intraperitoneal administration of doxorubicin; experimental group R2, in which animals after injection of doxorubicin, intragastrically administered the drug “Bendamine” at a dose of 20 mg/kg. According to studies, doxorubicin intoxication in rats enhances LPO processes, as indicated by an increased content of lipid hydroperoxides by 47.4 %, diene conjugates – by 21.4 % and TBA-active products – by 24.9 % for animals of the control group. Doxorubicin cardiomyopathy in rats has a decrease in the activity of antioxidant enzymes in the blood of animals. In particular, the activity of catalase decreases by 36.9 % (P ≤ 0.001), and the activity of SOD – by 20.3 % (P ≤ 0.001). In the study of the activity of the glutathione link of the antioxidant defense system, similar changes were found as in the study of the activity of catalase and SOD. The use of the drug “Bendamine” in the rats of the experimental group helped to inhibit the excessive formation of lipid peroxidation products and increase the activity of both enzymatic and non-enzymatic parts of the antioxidant system.
Grymak, Y., Skoromna, O., Stadnytska, O., Sobolev, O., Gutyj, B., Shalovylo, S., Hachak, Y., Grabovska, O., Bushueva, I., Denys, G., Hudyma, V., Pakholkiv, N., Jarochovich, I., Nahirniak, T., Pavliv, O., Farionik, Т., Bratyuk, V. (2020). Influence of “Thireomagnile” and “Thyrioton” preparations on the antioxidant status of pregnant cows. Ukrainian Journal of Ecology, 10(1), 122–126. doi: 10.15421/2020_19.
Gutyj, B. V., Gufriy, D. F., Binkevych, V. Y., Vasiv, R. O., Demus, N. V., Leskiv, K. Y., Binkevych, O. M., & Pavliv, O. V. (2018). Influence of cadmium loading on glutathione system of antioxidant protection of the bullocks’bodies. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnol-ogies, 20(92), 34–40. doi: 10.32718/nvlvet9207.
Kochie, S. L., Schober, K. E., Rhinehart, J., Winter, R. L., Bonagura, J. D., Showers, A., & Yildez, V. (2020). Effects of pimobendan on left atrial transport function in cats. J Vet Intern Med, 2020. doi: 10.1111/jvim.15976.
Martyshuk, T. V., & Gutyj, B. V. (2019). Influence of feed additive “Butaselmevit-Plus” on antioxidant sta-tus of rats in conditions of oxidative stress. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. Series: Agricultural sciences, 21(90), 76–81. doi: 10.32718/nvlvet-a9013.
Martyshuk, T. V., Gutyj, B. V., & Vishchur, O. I. (2018). Indicators of functional and antioxidant liver status of rats under oxidative stress conditions and on the action of the liposomal drug “Butaselmevit”. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 20(89), 100–107. doi: 10.32718/nvlvet8919.
Oldach, M. S., Ueda, Y., Ontiveros, E. S., Fousse, S. L., Harris, S. P., & Stern, J. A. (2019). Cardiac Effects of a Single Dose of Pimobendan in Cats With Hypertrophic Cardiomyopathy; A Randomized, Placebo-Controlled, Crossover Study. Front Vet Sci., 6, 15. doi: 10.3389/fvets.2019.00015.
Sarcinella, F., Neves, J., Maddox, T.W., Hodgkiss-Geere, H.M., Bode, E.F., Dukes-McEwan, J. (2020). Effect of pimobendan on left atrial function in dogs with preclinical myxomatous mitral valve disease. Open Vet J, 9(4), 375–383. doi: 10.4314/ovj.v9i4.16.
Tjostheim, S. S., Kellihan, H. B., Grint, K. A., & Stepien, R. L. (2019). Effect of sildenafil and pimobendan on intracardiac heartworm infections in four dogs. J Vet Cardiol., 23, 96–103. doi: 10.1016/j.jvc.2019.02.001.
Undhad, V. V., Fefar, D. T., Jivani, B. M., Gupta, H., Ghodasara, D. J., Joshi, B. P. & Prajapati, K. S. (2012). Cardiac troponin: an emerging cardiac biomarker in animal health. Vet. World, 5, 508–511. doi: 10.5455/vetworld.2012.508-511.
Varcholyak, I. S., & Gutyi, B. V. (2019). Determination of the chronic toxicity of preparation “Bendamin” on la-boratory animals. Theoretical and Applied Veterinary Medicine, 7(2), 63‒68. doi: 10.32819/2019.71011.
Varkholiak, I. S. (2016). The usage of medicines at the cardiovascular pathologies in dogs and cats. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 18, 3(71), 261–265. https://nvlvet.com.ua/index.php/ journal/article/view/974.
Varkholiak, I. S., & Gutyj, B. V. (2018). Determination of acute toxicity of “Bendamin” drug in laboratory animals. Scientific Messenger of Lviv National Uni-versity of Veterinary Medicine and Biotechnologies, 20(92), 209–212. doi: 10.32718/nvlvet9243.
Varkholiak, I. S., & Gutyj, B. V. (2019). The degree of cumulation of the “Bendamin” drug in the body of white rats. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnolo-gies. Series: Veterinary sciences, 21(94), 82–85. doi: 10.32718/nvlvet9415.
Varkholiak, I. S., & Gutyj, B. V. (2020). The influence of the preparation “Bendamin” on the morphological and biochemical indices of blood of rats in experimental modeling of heart failure. Ukrainian Journal of Veterinary and Agricultural Sciences, 3(1), 38–41. doi: 10.32718/ujvas3-1.07.
Vlizlo, V. V., Fedoruk, R. S., & Raty`ch, I. B. (2012). Laboratorni metody` doslidzhen` u biologiyi, tvary`nny`cztvi ta vetery`narnij medy`cy`ni: dovidny`k. L`viv: Spolom (in Ukrainian).
Yata, M., Kooistra, H. S., & Beijerink, N. J. (2019). Cardiorenal and endocrine effects of synthetic canine BNP1-32 in dogs with compensated congestive heart failure caused by myxomatous mitral valve disease. J Vet Intern Med., 33(2), 462–470. doi: 10.1111/jvim.15416.
Zhulikova, O. A. (2016). Monitoring rasprostranenija serdechno-sosudistyh zabolevanij sredi koshek i so-bak v g. Blagoveshhensk amurskoj oblasti. Dal'nevos-tochnyj agrarnyj vestnik, 2(38), 49–56. https://cyberleninka.ru/article/n/monitoring-rasprostraneniya-serdechno-sosudistyh-zabolevaniy-sredi-koshek-i-sobak-v-g-blagoveschensk-amurskoy-oblasti (in Russian).
Abstract views: 39 PDF Downloads: 17