The effect of sodium selenite and feed additive “Metisevit plus” on the protein-synthesizing function and functional state of the liver of rats under prolonged cadmium and lead loading


Keywords: pharmacology, toxicology, Cadmium, Lead, sodium selenite, Metisevit plus

Abstract

In the conditions of modern technogenic pollution of the environment, environmental problems, improving the quality of livestock products and their food safety is an important and urgent issue today. Pollution of the environment with heavy metals due to man-made activities of the population has led to a number of problems of agricultural production. The aim of the study was to investigate the effect of sodium selenite and feed additive Metisevit plus on the protein-synthesizing function and functional state of the liver of rats in lead-cadmium intoxication. It was found that the level of total protein in the blood of rats exposed to cadmium and lead was reduced. These changes were due to a decrease in albumin levels and an increase in globulin levels. Thus, hypoproteinemia resulting from cadmium and lead poisoning may indicate impaired hepatic protein synthesis. Transamination enzymes play a major role in the relationship between protein and carbohydrate metabolism, which is important for switching the oxidation of energy substrates from glucose to amino acid energy nutrition, which is extremely important under the influence of various environmental factors. The most active transaminases are aspartate aminotransferase and alanine aminotransferase. In the study of the activity of aminotransferases in the serum of rats treated with lead acetate and cadmium chloride, an increase in the activity of enzymes in all periods of the study. The highest activity of aminotransferases was on days 21 and 28 of the experiment in the control group of rats, which carried out the combined load of cadmium and lead. Thus, the load of the body of animals with cadmium and lead contributes to the violation of the functional state and protein-synthesizing function of the liver of animals of the experimental groups. With the introduction of sodium selenite into the body of rats, the activity of ALT and AST in the serum of the first experimental group was slightly reduced compared with the control group. However, the activity of these enzymes did not reach physiological values. When setting the feed additive Metisevit plus throughout the experiment, it was found that the activity of aminotransferases in the serum of rats of the second experimental group ranged within physiological values. This is due to the hepatoprotective effect of the components of the feed additive Metisevit Plus. In the study of protein-synthesizing function of rat liver in experimental lead-cadmium toxicosis, it was found that after application to rats of the first experimental group of sodium selenite on days 14, 21 and 28, the level of total protein in serum gradually increased, but did not reach physiological values. Using the feed supplement Metisevit plus rats of the second experimental group, found a slightly higher level of total protein in their blood, which on the 28th day of the experiment reached physiological values and, accordingly, amounted to 66.8 ± 1.99 g/l. It should be noted that when administering the feed additive Metisevit plus protein-synthesizing liver function and liver function of rats of the second experimental group were restored faster than when administering sodium selenite to the first experimental group.

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References

Al-Azemi, M., Omu, F. E., Kehinde, E. O., Anim, J. T., Oriowo, M. A., & Omu, A. E. (2010). Lithium protects against toxic effects of cadmium in the rat testes. J. Assist. Reprod. Genet, 27(8), 469–476. doi: 10.1007/s10815-010-9426-3.

Ali, M. M., Murthy, R. C., & Chandra, S. V. (1986). De-velopmental and longterm neurobehavioral toxicity of low–level in utero Cd exposure in rats. Neurobehavioral Toxicology and Teratology, 8 (5), 463–468. URL: https://www.ncbi.nlm.nih.gov/pubmed/3785508.

Chalaia, O. S. (2013). Vplyv riznykh rivniv kadmiiu ta svyntsiu v ratsioni molodniaku svynei na produktyv-nist i zabiini yakosti. Tavriiskyi naukovyi visnyk, 83, 196–202 (in Ukrainian).

Chen, Q. Y., DesMarais, T., & Costa, M. (2019). Metals and Mechanisms of Carcinogenesis. Annual Review of Pharmacology and Toxicology, 59(1), 537–554. doi: 10.1146/annurev-pharmtox-010818-021031.

Gutyi, B., Ostapiuk, A., Kachmar, N., Stadnytska, O., Sobolev, O., Binkevych, V., Petryshak, R., Petryshak, O., Kulyaba, O., Naumyuk, A., Nedashkivsky, V., Nedashkivska, N., Magrelo, N., Golodyuk, I., Nazaruk, N., & Binkevych, O. (2019). The effect of cadmium loading on protein synthesis function and functional state of laying hens’ liver. Ukrainian Journal of Ecology, 9(3), 222–226. URL: https://www.ujecology.com/articles/ the-effect-of-cadmium-loading-on-protein-synthesis-function-and-functional-state-of-laying-hens-liver.pdf.

Gutyj, B. V., Gufriy, D. F., Binkevych, V. Y., Vasiv, R. O., Demus, N. V., Leskiv, K. Y., Binkevych, O. M., & Pavliv, O. V. (2018). Influence of cadmium loading on glutathione system of antioxidant protection of the bullocks’bodies. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnol-ogies. Series: Veterinary sciences, 20(92), 34–40. doi: 10.32718/nvlvet9207.

Hutyi, B. V. (2015). Aktyvnist systemy antyoksydantnoho zakhystu orhanizmu bychkiv za hostroho kadmiievoho toksykozu. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii im. Gzhytskoho, 17, 1 (1), 31–36. URL: http://nbuv.gov.ua/UJRN/nvlnu_2015_17_1(1)__8 (in Ukrainian).

Kuras, L. D., & Ersteniuk, H. M. (2019). Pokaznyky ener-hetychnoho obminu v sertsevii tkanyni eksperymentalnykh tvaryn za umov vplyvu kadmii khlorydu. Medychna ta klinichna khimiia, 21 (1), 25–31. URL: http://nbuv.gov.ua/UJRN/Medkh_2019_21_1_6 (in Ukrainian).

Lavryshyn, Y. Y., & Gutyj, B. V. (2019). Protein synthe-size function of bulls liver at experimental chronic cadmium toxicity. Scientific Messenger of Lviv Na-tional University of Veterinary Medicine and Biotech-nologies. Series: Veterinary sciences, 21(94), 92–96. doi: 10.32718/nvlvet9417.

Lavryshyn, Y. Y., Gutyj, B. V., Palyadichuk, O. R., & Vishchur, V. Y. (2018). Morphological blood indices of bulls in experimental chronic cadmium toxicosis. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. Series: Veterinary sciences, 20(88), 108–114. doi: 10.32718/nvlvet8820.

Lavryshyn, Y. Y., Gutyj, B. V., Paziuk, I. S., Levkivska, N. D., Romanovych, M. S., Drach, M. P., & Lisnyak, O. I. (2019). The effect of cadmium loading on the activity of the enzyme link of the glutathione system of bull organism. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. Series: Veterinary sciences, 21(95), 107–111. doi: 10.32718/nvlvet9520.

López Alonso, M., & Benedit, J. L. (2002). Interactions Between Toxic and Essential Trace Metals in Cattle from a Region with Low Levels of Pollution. Archives of Environmental Contamination and Toxicology, 42(2), 165–172. doi: 10.1007/s00244-001-0012-7.

Marushko, Yu. V., Tarynska, O. L., Olefir, T. I., Fus, S. V. (2010). Kadmii: nakopychennia ta vplyv na orhanizm dytyny. Medychna nauka Ukrainy, 3, 62–67. URL: http://nbuv.gov.ua/UJRN/nvnmu_2010_3_12 (in Ukrainian).

Myslyva, T. M. (2013). Svynets i kadmii u hruntakh ahrolandshaftiv Zhytomyrskoho Polissia. Visnyk Sumskoho natsionalnoho ahrarnoho universytetu. Se-riia: Ahronomiia i biolohiia, 3, 43–50. URL: http://nbuv.gov.ua/UJRN/Vsna_agro_2013_3_13 (in Ukrainian).

Myslyva, T. M. (2013). Svynets i kadmii u hruntakh pryrodnykh i ahrolandshaftiv Zhytomyrskoho Polissia. Visnyk Zhytomyrskoho natsionalnoho ahroekolohichnoho universytetu, 1(1), 36–49. URL: http://nbuv.gov.ua/UJRN/Vzhnau_2013_1(1)__8 (in Ukrainian).

Ostapyuk, A. Y., & Gutyj, B. V. (2018). Influence of cadmium loading on morphological parameters of blood of the Laying Hens. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. Series: Veterinary sciences, 20(88), 48–52. doi: 10.32718/nvlvet8808.

Ostapyuk, A. Y., & Gutyj, B. V. (2019). Influence of cadmium sulfate at different doses on the functional state of the liver of laying chicken. Scientific Messen-ger of Lviv National University of Veterinary Medi-cine and Biotechnologies. Series: Veterinary sciences, 21(94), 103–108. doi: 10.32718/nvlvet9419.

Petrynych, V. V., Vlasyk, L. I., & Petrynych, O. A. (2017). Svynets: toksykolohichni, hihiienichni ta biolohichni aspekty. Klinichna ta eksperymentalna patolohiia, 16(2), 97–102. URL: http://nbuv.gov.ua/UJRN/ kep_2017_16_2_22 (in Ukrainian).

Slobodian, S. О., Gutyj, B. V., & Leskiv, K. Y. (2019). The level of lipid peroxidation products in the rats blood under prolonged cadmium and lead loading. Ukrainian Journal of Veterinary and Agricultural Sci-ences, 2(3), 15–18. doi: 10.32718/ujvas2-3.04.

Slobodian, S. О., Gutyj, B. V., & Murska, S. D. (2020). Effect of sodium selenite and feed additive “Metisevit plus” on morphological parameters of blood of rats at the intoxication of Cadmium and Lead. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. Series: Veterinary sci-ences, 22(97), 52–57. doi: 10.32718/nvlvet9710.

Stepanchuk, V. V. (2014). Ontohenetychni osoblyvosti tsyrkadiannykh khronorytmiv vilnoradykalnoho ho-meostazu za umov svyntsevoho otruiennia. Klinichna ta eksperymentalna patolohiia, 13(2), 131–133. URL: http://nbuv.gov.ua/UJRN/kep_2014_13_2_33 (in Ukrainian).

Vlizlo, V. V., Fedoruk, R. S., & Ratych, I. B. (2012). Laboratorni metody doslidzhen u biolohii, tvarynny-tstvi ta veterynarnii medytsyni. Dovidnyk. za red. Vlizla, V.V. Lviv. SPOLOM (in Ukrainian).

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Published
2020-12-23
How to Cite
Slobodian, S., Gutyj, B., Gufriy, D., Hnativ, P., & Murska, S. (2020). The effect of sodium selenite and feed additive “Metisevit plus” on the protein-synthesizing function and functional state of the liver of rats under prolonged cadmium and lead loading. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 22(100), 54-59. https://doi.org/10.32718/nvlvet10010

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