Nuclear morphometry of mammary tumors of dogs and histopathological diagnosis

Keywords: cancer, nucleus, area, perimeter, diameter.


For dog’s mammary tumors diagnostics scientists need researches, which can adopt and use methods developed for humans. The aim of this research paper is to set the inter relation between the parameters of nuclear morphometry (nuclear area, diameter, perimeter) and histopathological type of mammary tumors of dogs. Animals aged from 6 to 12 (medium meaning 9.2 ± 1.6 years). According to histopathological research 3 tumors were benign and 25 were malignant, 18 of them – malignant epithelial neoplasms (3 tubular carcinomas, 13 tubulopapillary carcinomas, 1 cystic-papillary carcinoma, 3 colid carcinomas, 1 micropapillary carcinoma), 3 malignant epithelial neoplasms of special type (mucinous, lipid-rich, spindle cell carcinomas) and 4 malignant mesenchymal neoplasms (chondrosarcoma). Nuclear morphometry parameters (nuclear area, perimeter and diameter) of benign tumours (20.48 ± 1.22, 19.13–21.50 µm2; 19.27 ± 0.10, 19.17–19.36 µm and 5.09 ± 0.16, 4.92–5.22 µm) were probably smaller than in malignant tumours, for example in simple carcinoma (38.61 ± 5.61, 29.26–46.16 µm2; 26.42 ± 2.32, 22.10–29.60 µm and 6.96 ± 0.52, 6.03–7.62 µm), tubular (37.89 ± 7.30, 29.94–46.16 µm2, 26.34 ± 2.83, 22,98–29,60 µm and 6.90 ± 0.70, 6.16–7.62 µm), tubulopapillary (40.22 ± 3.48, 34.38–44.75 µm2, 27.02 ± 1.49, 24.41–28.97 µm and 7.12 ± 0.31, 6.58–7.52 µm), colid (43.57 ± 5.54, 37.71–48.73 µm2, 28.05 ± 1.88, 26.54–30.15 µm and 7.41 ± 0.47, 6.91–7.85 µm), other malignant epithelial neoplasms (39.99 ± 5.15, 29.94–48.73 µm2; 26.85 ± 2.03, 22.98–30.15 µm and 7.09 ± 0.50, 6.16–7.85 µm) and malignant epithelial neoplasms of special types (45.89 ± 4.12, 43.41–50.65 µm2; 29.92 ± 0.21, 29.68–30.06 µm and 7.60 ± 0.34, 7.41–8.00 µm). However, there was not statistically significant difference in comparison between benign tumours and sarcomas (25.95 ± 5.21, 21.64–33.00 µm2; 21.85 ± 1.79, 20.21–24.05 µm and 5.68 ± 0.56, 5.21–6.42 µm). Among the different groups of malignant neoplasms lower rates were in sarcoma, the other groups had no difference. Taking into consideration the indicators of nuclear morphometry (nuclear area, diameter and perimeter) different types of neoplasms can be differentiated: benign from malignant tumours and sarcomas from malignant epithelial neoplasms (tubular, tubulopapillary, cystic-papillary, colid, micropapillary, mucinous, lipid-rich and spindle cell carcinomas).


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Fedets, O., Zayats, O., Zaitsev, O., Zakrevska, M., Kurlyak, I., & Midyanyy, S. (2020). Nuclear morphometry of mammary tumors of dogs and histopathological diagnosis. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 22(100), 123-128.