The intensity of the processes of lipid peroxidation in the blood of cows in experimental fasciolosis
Among the most common pathologies of parasitic etiology in ruminants, trematode diseases, namely fasciolosis, occupy a prominent place in terms of the degree of damage and economic damage caused. Activation of lipid peroxidation processes plays a significant role in the mechanisms of the development of fasciolosis in animals. The research aimed to investigate the level of lipid peroxidation products in cows' blood in experimental fasciolosis. For the test, 12 cows of 4–5 years of age, black-spotted breed, were selected, from which 2 groups were formed, six animals in each. Animals in the control group were clinically healthy. Animals of the experimental group were experimentally infected with adolescents. During the research, the rules of compulsory experiments were followed – selecting and keeping analog animals in groups. The cows' diet was balanced in terms of nutrients and minerals. Based on the studies, it was found that after the experimental infestation of cows of the experimental group with the causative agent of fasciolosis, an increase in the level of diene conjugates of their blood on the 15th day of the experiment by 30.3 %, and on the 25th day – by 50.4 % relative to the control group. There was also an increased level of lipid hydroperoxides in their blood, where, respectively, on the 10th and 15th day of the experiment, this figure increased by 18.3 and 32.9 % compared with the control group. On the 25th day of the experiment, the level of lipid hydroperoxides in the blood of cows of the experimental group was the highest. Compared with the control group, it increased by 46.6%, respectively. In the study of the final products of lipid peroxidation, it was found that on the 10th day of the test, the level of TBA-active products in infected cows of the experimental group increased by 9.5 %. On the 15th and 20th days of the experiment, the level of end products of LPO in the experimental group's blood of animals was higher by 20.3 and 26.0 % relative to the control group. The increase in TBA-active products' level reflects the activation of peroxide processes in animals with experimental fasciolosis. Therefore, in the clinical manifestation of fasciolosis, parasites secrete metabolic products that promote the formation of free radicals, which in turn enhance the initiation of lipid peroxidation processes.
Chernushkin, B. O., Vlizlo, V.V., Slivinska, L. G., Gutyj, B. V., Shcherbatyy, A. R., Maksymovych, I. A., Leno, M. I., Rusyn, V. I., Lychuk, M. H., Fedorovych, V. L., Lukashchuk, B. O., Zinko, H. O., & Prystupa, O. I. (2020). Treatment strategies for sheep with acute yel-low athrophy of the liver caused by the fasciolosis. Ukrainian Journal of Ecology, 10(2), 294–301. doi: 10.15421/2020_100.
Cunha-Oliveira, T., Montezinho, L., Mendes, C., Firuzi, O., Saso, L., Oliveira, P. J., & Silva, F. S. G. (2020). Oxidative Stress in Amyotrophic Lateral Sclerosis: Pathophysiology and Opportunities for Pharmacological Intervention. Oxid Med Cell Longev, 2020, 5021694. doi: 10.1155/2020/5021694.
Grymak, Y., Skoromna, O., Stadnytska, O., Sobolev, O., Gutyj, B., Shalovylo, S., Hachak, Y., Grabovska, O., Bushueva, I., Denys, G., Hudyma, V., Pakholkiv, N., Jarochovich, I., Nahirniak, T., Pavliv, O., Farionik, Т., & Bratyuk, V. (2020). Influence of “Thireomagnile” and “Thyrioton” preparations on the antioxidant status of pregnant cows. Ukrainian Journal of Ecology, 10(1), 122–126. doi: 10.15421/2020_19.
Gutyj, B., Khariv, I., Binkevych, V., Binkevych, O., Levkivska, N., Levkivskyj, D., & Vavrysevich, Y. (2017). Research on acute and chronic toxity of the experimental drug Аmprolinsyl. Regulatory Mechanisms in Biosystems, 8(1), 41–45. doi: 10.15421/021708.
Kausar, S, Wang, F, & Cui, H. (2018). The role of mitochondria in reactive oxygen species generation and Its implications for neurodegenerative diseases. Cells, 7(12), 274. doi: 10.3390/cells7120274.
Kulyaba, O. V., Stybel, V. V., & Gutyj, B. V. (2016). The influence of clozaverm A and catozal on antioxidant status of cows organism for the experimental fasciolosis, sensitized atypical mycobacteria. Scientific Messenger LNUVMB named after S. Z. Gzhytskyj, 18, 2(66), 96–99. doi: 10.15421/nvlvet6621.
Kulyaba, O., Stybel, V., & Gutyj, B. (2017). The influence of closaverm а and catosal on indicators of protein synthesizing functions of cows liver by experimental fasciolosis, sentsitized by atypical mycobacteria. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 19(73), 122–125. doi: 10.15421/nvlvet7325.
Kulyaba, O., Stybel, V., Gutyj, B., Turko, I., Peleno, R., Turko, Ya., Golovach, P., Vishchur, V., Prijma, O., Mazur, I., Dutka, V., Todoriuk, V., Golub, O. Dmytriv, O., & Oseredchuk, R. (2019). Effect of experimental fascioliasis on the protein synthesis function of cow liver. Ukrainian Journal of Ecology, 9(4), 612–615. URL: https://www.ujecology.com/articles/effect-of-experimental-fascioliasis-on-the-protein-synthesis-function-of-cow-liver.pdf.
Lesyk, Y., Ivanytska, A., Kovalchuk, I., Monastyrska, S., Hoivanovych, N., Gutyj, B., Zhelavskyi, M., Hulai, O., Midyk, S., Yakubchak, O., & Poltavchenko,T. (2020). Hematological parameters and content of lipids in tissues of the organism of rabbits according to the silicon connection. Ukrainian Journal of Ecology, 10(1), 30–36. doi: 10.15421/2020_5.
Said, W., Stybel, V., Gutyj, B., & Prijma, O. (2020). Antioxidant protection system of dog organism at experimental toxocariasis. Bulletin of Poltava State Agrarian Academy, 3, 233–240. doi: 10.31210/visnyk2020.03.27.
Slivinska, L. G., Shcherbatyy, A. R., Lukashchuk, B. O., & Gutyj, B. V. (2020). The state of antioxidant protec-tion system in cows under the influence of heavy met-als. Regulatory Mechanisms in Biosystems, 11(2), 237–242. doi: 10.15421/022035.
Vlizlo, V. V. (2012). Laboratorni metody doslidzhen u biologiyi, tvarynnycztvi ta veterynarnij medycyni: dovidnyk. Lviv: Spolom (in Ukrainian).
Abstract views: 18 PDF Downloads: 12