Determination of acute and subacute toxicity of the drug “Tilovet 20 %”


  • V. I. Kushnir State Scientific-Research Control Institute of Veterenary Medicinal Products and Feed Additives, Lviv, Ukraine https://orcid.org/0000-0002-9374-2454
  • I. P. Patereha State Scientific-Research Control Institute of Veterenary Medicinal Products and Feed Additives, Lviv, Ukraine https://orcid.org/0000-0003-4747-9365
  • I. M. Kushnir State Scientific-Research Control Institute of Veterenary Medicinal Products and Feed Additives, Lviv, Ukraine https://orcid.org/0000-0001-6117-9735
  • B. V. Gutyj Stepan Gzhytskyj National University of Veterinary Medicine and Biotechnologies Lviv, Ukraine https://orcid.org/0000-0002-5971-8776
  • S. Yа. Martynyk State Scientific-Research Control Institute of Veterenary Medicinal Products and Feed Additives, Lviv, Ukraine https://orcid.org/0000-0002-5163-5976
  • М. М. Chudyak State Scientific-Research Control Institute of Veterenary Medicinal Products and Feed Additives, Lviv, Ukraine https://orcid.org/0000-0001-5298-2876
  • Т. V. Yurynets State Scientific-Research Control Institute of Veterenary Medicinal Products and Feed Additives, Lviv, Ukraine
  • V. A. Smuk State Scientific-Research Control Institute of Veterenary Medicinal Products and Feed Additives, Lviv, Ukraine
  • A. S. Kishko State Scientific-Research Control Institute of Veterenary Medicinal Products and Feed Additives, Lviv, Ukraine
Keywords: acute toxicity, subacute toxicity, laboratory animals, “Tilovet 20 %”, hematological and biochemical parameters.

Abstract

The article presents the results of determination of acute and subacute toxicity of the drug “Tilovet 20 %”, which was made on the basis of tylosin tartrate. In result of the conducted researches, it was found out, that LD50 of the drug “Tilovet 20 %” by intramuscular injection to white mice (calculated by the method of G. Kerber) is 10000 and mg/kg, and for white rats 9583.33 mg/kg. Long-term use of the drug in a therapeutic dose caused a tendency to the decrease of hemoglobin concentration, amount of erythrocytes, platelets, hematocrit value, mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), content of the total protein, creatinine level, ALP activity on the background of a slight an increase of amount of leukocytes, urea level, AST and ALT activity compared to the values of the control group. The use of the drug in a dose that is 10 times higher than the therapeutic, caused a probable decrease of hemoglobin concentration by 6.6 % (P < 0.05), platelet count by 32.5 % (P < 0.05), mean corpuscular hemoglobin (MCH) by 4.2 % (P < 0.05), mean corpuscular hemoglobin concentration (MCHC) by 1.5 % (P < 0.1), hematocrit value by – 4.95 %, and total protein content by – 10.7 % (P < 0.05), on the background of a slight an increase of amount of leukocytes by 5.3 %, ALP activity by – 17.5 %, compared to the values oft he control group. Therefore, all of the above may indicate to the suppression of hematopoietic processes and a decrease in the protein-synthesizing function of the liver.

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Published
2021-04-05
How to Cite
Kushnir, V., Patereha, I., Kushnir, I., Gutyj, B., Martynyk, S., ChudyakМ., YurynetsТ., Smuk, V., & Kishko, A. (2021). Determination of acute and subacute toxicity of the drug “Tilovet 20 %”. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 23(101), 15-20. https://doi.org/10.32718/nvlvet10103