The effectiveness of the proposed method of culturing eggs of nematodes Passalurus ambiguus, parasitic in rabbits
Rabbit breeding is one of the promising branches of meat cattle breeding, and also plays an important role in supplying light industry with fur raw materials. One of the factors that ensure the efficient management of the industry and increase the production of rabbit products is the veterinary welfare of farms against parasitic diseases of rabbits, in particular – pasalurosis. It is necessary to prevent the development of the parasite at all stages in order to successfully control and prevent pasalurosis. The aim of the work was to improve and determine the effectiveness of the method of cultivation of Passalurus ambiguus eggs, which can be used to study the embryogenesis of the pathogen pasalurosis in rabbits, obtain a laboratory model of invasion, determine the disinvasive properties of disinvasive and disinfectants and agents. The proposed method of culturing P. ambiguus nematode eggs allows to take into account the sensitivity of eggs to moisture, allows to obtain a high yield of invasive eggs in the experimental culture, as well as to conduct microscopic examinations and microphotography without removing eggs from the substrate in which cultivation takes place. Eggs of P. ambiguus were obtained from the gonads of females of the pathogen pasalurosis, which were removed by helminthic dissection of the colon of rabbits. The proposed method is based on the use of nutrient environment for the cultivation of P. ambiguus eggs. Studies have shown that the improved method was more effective than the well-known method, where as a substrate for the cultivation of Pasaluris eggs used saline. With the use of the improved method, the invasive stage reaches more than 71 % of Pasaluris eggs, which is 38.75% more (P < 0.001) than with the use of the well-known method of cultivation. The obtained results of the conducted research activities lead to further, deeper study of questions concerning biological features of embryonic development of the causative agent of passalurosis, and also disinvasive efficiency of modern disinfectants concerning P. ambiguus eggs.
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