The efficiency of the drug based on butaphosphane, B vitamins and L-carnitine in the treatment of horses
An important feature of living organisms, acquired in the process of evolution, is the ability to adapt to various external influences and changes in the environment, to maintain homeostasis and regulatory processes in organs, systems and the body as a whole. However, with modern breeding technologies, the physiological capabilities of the animal's body are often unable to change as quickly as required by the conditions of industrial maintenance, which leads to stress. Vitamin preparations and biologically active feed additives are widely used by veterinary medicine specialists to increase resistance to stressful situations, disease prevention, increase resistance, safety and productivity of farm animals. Preparation L-tsyn, solution for injection (manufactured by BIOTESTLAB LLC), is a drug, the action of which is due to the prescription combination of active substances, namely: butaphosphane, B vitamins (vitamin B12, vitamin B3) and L-carnitine. The aim of the study was to investigate the effectiveness of the drug L-tsyn on horses under the influence of stressors of various natures. Two experiments on working horses with constant, excessive physical activity with chronic exercises and with complex therapy of intoxication with signs of acute renal failure in horse were conducted. Clinical trials of the drug were performed on working horses aged 5–15 years, of different sexes. The drug was administered in a dose of 20–25 ml per animal, 1 time per day for 5 consecutive days. The clinical condition of the experimental animals was observed for 21 days from the start of the drug. The morpho-functional state of the animal's body before and after the drug was established according to the indicators of the clinical condition, morphological and biochemical parameters of the blood, which were determined according to generally accepted methods. In working horses under excessive load the drug L-tsyn activated the processes of hematopoiesis, improved the physiological state of the body and contributed to the increase the general tone of the body. The drug as part of complex therapy in the treatment of intoxication, showed hepato- and nephroprotective properties, reduced the manifestations of intoxication, helped to restoration of the animal’s normal physiological state. According to the results of clinical, hematological and biochemical studies, it can be concluded that the drug L-tsyn has a stimulating effect on metabolic processes in the animals’ body, shows tonic properties, increases the body's resistance to toxins and adverse environmental factors and can be used as monotherapy and for the complex treatment of diseases of various etiologies in horses.
Directive 81/852/EEC and EU guideline “Good clinical practice clinical trials on veterinary medicinal products in the European Union”.
European Treaty Series/123. European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes. Strasbourg, 18.III.1986. URL: https://rm.coe.int/168007a67b.
Grymak, Y., Skoromna, O., Stadnytska, O., Sobolev, O., Gutyj, B., Shalovylo, S., Hachak, Y., Grabovska, O., Bushueva, I., Denys, G., Hudyma, V., Pakholkiv, N., Jarochovich, I., Nahirniak, T., Pavliv, O., Farionik, Т., & Bratyuk, V. (2020). Influence of “Thireomagnile” and “Thyrioton” preparations on the antioxidant sta-tus of pregnant cows. Ukrainian Journal of Ecology, 10(1), 122–126. doi: 10.15421/2020_19.
Horbatiuk B. I. (2004). Metodychni rekomendatsii z diahnostyky ta doslidzhennia zahalnoho stanu orhanizmu tvaryn. Lviv (in Ukrainian).
Hrabovskyi, S. S. (2012). Stresy silskohospodarskykh tvaryn ta yoho naslidky. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii imeni S. Z. Hzhytskoho, 14(3(53), 47–58 (in Ukrainian).
Kotsiumbas, I. Ya, Bisiuk, I. Yu., Horzheiev, V. M., & Malyk O. H. (2013). Klinichni doslidzhennia veterynarnykh preparativ ta kormovykh dobavok. L.:TOV Vydavnychyi dim “SAM” (in Ukrainian).
Kotsiumbas, I. Ya., Malyk, O. H., & Patereha, I. P. (2006). Doklinichni doslidzhennia veterynarnykh likarskykh zasobiv. Lviv: Triada plius (in Ukrainian).
Kotsiumbas, I. Ya., Zhyla, M. I., & Pyatnychko, O. M. (2014). Imunotoksykolohichnyi kontrol veterynarnykh preparativ ta kormovykh dobavok: Metodychni rekomendatsii. Lviv (in Ukrainian).
Kreipe, L., Deniz, A., Bruckmair, R. V., & van Dorland, H. A. (2011). First report about the mode of action of combined butafosfan and cyanocobalamin on hepat-ic metabolism in nonketotic early lactating cows. Journal of dairly science, 94(10), 4904–4914. doi: 10.3168/jds.2010-4080.
Lapovets, L. Ye., Lutsyk, B. D., & Lebed, H. B. (2011). Klinichna laboratorna diahnostyka. Praktykum. Lviv (in Ukrainian).
Leskiv, Kh Ya., Gufriy, D. F., Gutyj, B. V., Khalak, V. I., Hariv, I. I., Martyshuk, T. V., & Guta, Z. A. (2021). The effect of methiphene, methionine, and phenarone on the humoral part of the immune System of piglets in experimental chronic nitrate-nitrite toxicosis. Colloquium-journal, 7(94), 12–15. doi: 10.24412/2520-6990-2021-794-12-15.
Levchenko, V. I., Vlizlo, V. V., & Kondrakhin, I. P. (2002). Veterynarna klinichna biokhimiуa. Bila Tserkva (in Ukrainian).
Levchenko, V. Y., Kondrakhyn, Y. P., Sudakov, N. A., & Chumachenko, V. Iu. (1999). Vnutrennye bolezny zhyvotnykh. Belaya Tserkov (in Russian).
Lourens, D. R., & Benyt. P. N. (1991). Klynycheskaia farmakolohyia. M.: Medytsyna (in Russian).
Martyshchuk, T. V., & Gutyi, B. V. (2019). Influence of feed additive “Butaselmevit Plus” on the indicators of rats blood under the conditions of their poisoning with Tetrachloromethane. Theoretical and Applied Veteri-nary Medicine, 7(2), 79‒83. doi: 10.32819/2019.71014.
Martyshuk, T. V., & Gutyj, B. V. (2019). Influence of feed additive “Butaselmevit-Plus” on antioxidant status of rats in conditions of oxidative stress. Scien-tific Messenger of Lviv National University of Veteri-nary Medicine and Biotechnologies. Series: Agricul-tural sciences, 21(90), 76–81. doi: 10.32718/nvlvet-a9013.
Martyshuk, T. V., Gutyj, B. V., & Vishchur, O. I. (2019). Morphological and biochemical indices of piglets' blood by the action of feed additive “Butaselmevit-plus”. The Animal biology, 21(4), 65–70. doi: 10.15407/animbiol21.04.065.
Martyshuk, T. V., Gutyj, B. V., Vishchur, O. I., & Todori-uk, V. B. (2019). Biochemical indices of piglets blood under the action of feed additive “Butaselmevit-plus”. Ukrainian Journal of Veterinary and Agricultural Sci-ences, 2(2), 27–30. doi: 10.32718/ujvas2-2.06.
Martyshuk, T. V., Gutyj, B. V., Zhelavskyi, M. M., Midyk, S. V., Fedorchenko, A. M., Todoriuk, V. B., Nahirniak, T. B., Kisera, Ya. V., Sus, H. V., Chemerys, V. A., Lev-kivska, N. D., & Iglitskej, I. I. (2020). Effect of Bu-taselmevit-Plus on the immune system of piglets dur-ing and after weaning. Ukrainian Journal of Ecology, 10(2), 347–352. doi: 10.15421/2020_106.
Nazaruk, N. V., Gutyj, B. V., Gufrij, D. F., Leskiv, Kh. Ya., Ivashkiv, R.M., & Martyshuk, T. V. (2021). The effect of methyphen and vitamix se on the level of products of bull lipid peroxide oxidation under nitrate-cadmium load. Colloquium-journal, 7(94), 16-18. doi: 10.24412/2520-6990-2021-794-16-18.
Nelson, D. L., & Cox, M. M. (2017). Lehninger principles of biochemistry. New York: W. H. Freeman.
Nuber, U., van Dorland, H. A., & Bruckmaier, R. M. (2015). Effects of butafosfan with or without cyano-cobalamin on the metabolism of early lactating cows with subclinical ketosis. J. Anim. Physiol. Anim. Nutr. (Berl.), 100(1), 146–155. doi: 10.1111/jpn.12332.
Saliy, O., Baula, N., & Sopizhenko, O. (2020). The analy-sis of the regulatory and legal support for clinical trials of medicines and veterinary preparations in Ukraine and the EU. Management, economy and guality as-surance in pharmacy, 4(6), 36–44. doi: 10.24959/uekj.20.34.
Slivinska, L. G., Shcherbatyy, A. R., Lukashchuk, B. O., & Gutyj, B. V. (2020). The state of antioxidant protec-tion system in cows under the influence of heavy metals. Regulatory Mechanisms in Biosystems, 11(2), 237–242. doi: 10.15421/022035.
Suprun, I. O. (2012). Henezys stresu silskohospodarskykh tvaryn. Biologia tvaryn, 14(1-2), 55–63 (in Ukraini-an).
Tarasenko, L. M., Hryhorenko, V. K., & Neporada, K. S. (2007). Funktsionalna biokhimiia. Vinnytsia (in Ukrainian).
VICH (2000). VICH GL9: Good clinical practices. URL: https://vichsec.org/en.
VICH (2008). VICH GL43. Target Animal Safety for Veterinary Pharmaceutical Products. URL: https://vichsec.org/en.
Vlizlo, V. V., Fedoruk, R. S., & Ratych, I. B. (2012). Laboratorni metody doslidzhennia v biolohii, tvarynnytstvi ta veterynarnii medytsyni: dovidnyk. Lviv: Spolom (in Ukrainian).
Zhyla, M. I. (2011). Laboratorni doslidzhennia pry klinichnomu vyprobuvanni veterynarnykh likarskykh zasobiv. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii imeni S. Z. Hzhytskoho 13, 4(50), 128–134 (in Ukrainian).
Zhyla, M. I., Piatnychko, O. M., & Shkodiak, N. V. (2015). Terapevtychna efektyvnist veterynarnoho likarskoho zasobu Vetozal 10% pry likuvanni porosyat z oznakamy anemii. Naukovo-tekhnichnyi biuleten DNDKI vetpreparativ ta kormovykh dobavok i Instytutu biolohii tvaryn, 16(2), 134–139 (in Ukrainian).
Zhyla, M. I., Piatnychko, O. M., & Shkodiak, N. V. (2016). Kontrol yakosti henerychnykh veterynarnykh likarskykh zasobiv. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii imeni S.Z. Hzhytskoho, 18, 1(65), 35–41 (in Ukrainian).
Abstract views: 18 PDF Downloads: 6