Sexual cycle of cats and non-invasive diagnostic methods

  • O. V. Burlakova Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, Ukraine
  • V. Y. Stefanyk Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, Ukraine
Keywords: wild feline, cat, feline reproduction, sexual cycle of cats, non-invasive diagnostic methods, sex hormones.


Mostly all feral cats are listed in the IUCN International Red Book. This fact is caused by the negative impact of civilization on the ecosystem, including fauna. This trend applies equally to feral cats from the family Felidae (tigers, jaguars, leopards, snow leopards) and the family Felinae (caracal, serval, ocelot, etc.). The low population size of domestic species, namely lynx and forest cat in Ukraine, is alarming. The use of innovative biotechnological techniques in reproduction can have a positive effect on the conservation of endangered carnivorous species. These auxiliary methods are aimed at improving the technique of obtaining and cryopreservation of sperm, oocytes, in vitro fertilization, artificial insemination, embryo transplantation, as well as synchronization and regulation of the sexual cycle. This last element is key in ex situ conservation programs in zoos. Under physiological course, feline reproductive cycle is unique, as the physiology of the body as a whole. The wild cat family is the pinnacle of predator evolution. Today there are 39 species of this family in the world, including the domestic cat. Most feral cats are endangered. The main reasons for the decline in wild cat populations in the wild are habitat loss, fragmentation, human-animal conflict, and for some species, poaching for fur and medicine. Therefore, many scientists in the world face the issue of increasing reproductive capacity, improving methods of monitoring reproductive function and endocrine status, as different species of cats have significant differences in the sexual cycle. The main purpose of the work is to improve the reproductive properties in the reproduction of wild cats using non-invasive diagnostic methods. Reproduction is a key success factor, so understanding the basics of the reproduction function will help develop strategies to conserve and influence populations of different feline species.


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Adachi, I., Kusuda, S., Kawai, H., Ohazama, M., Tanigu-chi, A., Kondo, N., Yoshihara, M., Okuda, R., Ishikawa, T., Kanda, I., & Doi, O. (2011). Fecal Pro-gestagens to Detect and Monitor Pregnancy in Cap-tive Female Cheetahs (Acinonyx jubatus). Journal of Reproduction and Development, 57(2), 262–266. doi: 10.1262/jrd.10-057T.

Banks, D. H., Paape, S. R., & Stabenfeldt, G. H. (1983). Prolactin in the cat: I. Pseudopregnancy, pregnancy and lactation. Biol. Reprod., 28(4), 923–932. doi: 10.1095/biolreprod28.4.923.

Beehner, J. C., & Whitten, P. L. (2004). Modifications of a field method for fecal steroid analysis in baboons. Physiology & Behavior, 82(2–3), 269–277. doi: 10.1016/j.physbeh.2004.03.012.

Brown, J. L. (2006). Comparative endocrinology of do-mestic and nondomestic felids. Theriogenology, 66(1), 25–36. doi: 10.1016/j.theriogenology.2006.03.011.

Brown, J. L. (2011). Female reproductive cycles of wild female felids. Anim Reprod Sci., 124(3-4), 155–162. doi: 10.1016/j.anireprosci.2010.08.024.

Brown, J. L., Wasser, S. K., Wildt, D. E., & Graham, L. H. (1994). Comparative aspects of steroid hormone me-tabolism and ovarian activity in felids, measured non-invasively in feces. Biol Reprod., 51(4), 776–786. doi: 10.1095/biolreprod51.4.776.

Djul'ger, G. P. (2004). Akusherstvo, ginekologija i biotehnika razmnozhenija koshek. Uchebnoe posobie. M.: Kolos (in Russian).

England, G., & von Heimendahl, A. (2010). BSAVA Manual of Canine and feline reproduction and neo-natology. Secondedition, 8–12.

Graham, L., Schwarzenberger, F., Möstl, E., Galama, W., & Savage, A. (2001). A versatile enzyme immunoas-say for the determination of progestogens in feces and serum. Zoobiology, 20(3), 227–236. doi: 10.1002/zoo.1022.

Howard, J. G., Barone, M. A., Donoghue, A. M., & Wildt, D. E. (1992). The effect of preovulatory anaesthesia on ovulation in laparoscopically inseminated domes-tic cats. J. Reprod. Fertil., 96(1), 175–186. doi: 10.1530/jrf.0.0960175.

Howard, J. G., Byers, A. P., Brown, J. L., Schwartz, R. J., Evans, M. Z., & Barrett, S. J. (1996). Successful ovula-tion induction and laparoscopic intrauterine artificial insemination in the clouded leopard (Neofelis nebu-losa). Zoo Biol., 15, 55–69. doi: 10.1095/biolreprod56.4.1059.

Johnson, L. M., & Gay, V. L. (1981). Luteinizing hormone in the cat. I. Tonic secretion. Endocrinology, 109(1), 240–246. doi: 10.1210/endo-109-1-240.

Kumar, V., Reddy, V. P., Kokkiligadda, A., Shivaji, S., & Umapathy, G. (2014). Non-invasive assessment of re-productive status and stress in captive Asian ele-phants in three south Indian zoos. Gen Comp Endo-crinol, 201, 37–44. doi: 10.1016/j.ygcen.2014.03.024.

Lasley, B. L., & Kirkpatrick, J. F. (1991). Monitoring Ovar-ian Function in Captive and Free-Ranging Wildlife by Means of Urinary and Fecal Steroids. Journal of Zoo and Wildlife Medicine, 22(1), 23–31. URL:

Najdenko, S. V. (2015). Biologija razmnozhenija koshachih: Mehanizmy povyshenija reproduktivnogo uspeha. Dissertacija na soiskanie uchenoj stepeni doktora biologicheskih nauk. Moskva (in Russian).

Nowell, K., & Jackson, P. (1996). Wild Cats. Status Survey and Conservation Action Plan. IUCN/SSC Cat Spe-cialist Group. IUCN, Gland. URL: library/node/6998.

Pavlova, E. V., & Najdenko, S. V. (2008). Neinvazivnyj monіtoring gljukokortikoidov v ekskrementah dal'-nevostochnogo lesnogo kota (Prionailurus bengalensis euptilurus). Zoologicheskijzhurnal, 87(11), 1375–1381 (in Russian).

Putranto, H. D. (2011). A non-invasive identification of hormone metabolites, gonadal event and reproductive status of captive female tigers. Biodiversitas Journal of Biological Diversity, 12(3). doi: 10.13057/biodiv/ d120302.

Putranto, H. D., Kusuda, S., Hashikawa, H., Kimura, K., Naito, H., & Doi, O. (2007). Fecal Progestins and Es-trogens for Endocrine Monitoring of Ovarian Cycle and Pregnancy in Sumatran Orangutan (Pongo abelii). Japanese Journal of Zoo and Wildlife Medicine, 12(2), 97–103. doi: 10.5686/jjzwm.12.97.

Putranto, H. D., Kusuda, S., Ito, T., Terada, M., & Inagaki, K. (2007). Reproductive Cyclicity Based on Fecal Steroid Hormones and Behaviors in Sumatran Tigers, Panthera tigris sumatrae. Japanese Journal of Zoo and Wildlife Medicine, 12(2), 111–115. doi: 10.5686/jjzwm.12.111.

Say, L., Devillard, S., Natoli, E., & Pontier, D. (2002). The mating system of feral cats (Felis catus L.) in a sub-Antarctic environment. Polar Biology, 25, 838–842. doi: 10.1007/s00300-002-0427-2.

Schwarzenberger, F., Möstl, E., Palme, R., & Bamberg, E. (1996). Faecal steroid analysis for non-invasive monitoring of reproductive status in farm, wild and zoo animals. Animal Reproduction Science, 42(1–4), 515–526. doi: 10.1016/0378-4320(96)01561-8.

Schwarzenberger, J. C., & Gogarten, W. (2007). Anesthe-sia for patients with congenital heart disease undergo-ing non-cardiac surgery. Anasthesiol Intensivmed Notfallmed Schmerzther, 42(11), 822–832. doi: 10.1055/s-2007-1003596.

Shille, V. M., Munro, C., Farmer, S. W., & Papkoff, H. (1983). Ovarian and endocrine responses in the cat af-ter coitus. J. Reprod. Fertil., 69(1), 29–39. doi: 10.1530/jrf.0.0690029.

Stewart, D. R., & Stabenfeldt, G. H. (1985). Relaxin activi-ty in the pregnant cat. Biol Reprod, 32(4), 848–854. doi: 10.1095/biolreprod32.4.848.

Touma, C., & Palme, R. (2005). Measuring fecal gluco-corticoid metabolites in mammals and birds: the im-portance of validation. Annals of the New York Academy of Sciences, 1046(1), 54–74. doi: 10.1196/annals.1343.006.

Trigo, T. C., Schneider, A., de Oliveira, T. G., Lehugeur, L. M., Silveira, L., Freitas T. R. O., & Eizirik, E. (2013). Molecular data reveal complex hybridization and a cryptic species of neotropical wild cat. Curr Biol., 23(24), 2528–2533. doi: 10.1016/j.cub.2013.10.046.

Umapathy, G., Kumar, V., Wasimuddin, Kabra, M., & Shivaji, S. (2013). Detection of pregnancy and fertility status in big cats using an enzyme immunoassay based on 5α-pregnan-3α-ol-20-one. Gen Comp Endo-crinol, 180, 33–38. doi: 10.1016/j.ygcen.2012.10.009.

Wildt, D. E., Brown, J. L., & Swanson, W. F. (1998). Re-production in cats. In: Knobil, E., Neill, J. (Eds.), Ency-clopedia of Reproduction, 1st ed. Academic Press, New York, 497–510.

Wilting, A., Buckley-Beason, V. A., Feldhaar, H., Gadau, J., O'Brien, S. J., & Eduard Linsenmair K. (2007). Clouded leopard phylogeny revisited: support for spe-cies recognition and population division between Bor-neo and Sumatra. Front Zool., 4, 15. doi: 10.1186/1742-9994-4-15.

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How to Cite
Burlakova, O., & Stefanyk, V. (2021). Sexual cycle of cats and non-invasive diagnostic methods. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 23(101), 124-132.