Staphylococcus aureus of raw cow's milk

Keywords: cow's milk, seeding, Staphylococcus aureus, skin of teats, milking equipment.


The presence of Staphylococcus aureus in raw milk is considered a direct danger to consumers. Almost all types of dairy products normalize the amount of Staphylococcus aureus in 1 g (cm3) of product, due to their ability to produce thermostable enterotoxins. The aim of the study was to investigate the role of incision of teats of healthy and sick mastitis cows and milking equipment for irradiation of cow's milk with Staphylococcus aureus. Sanitation of milking equipment in farms was carried out with alkaline detergents based on sodium hypochlorite and acid based on phosphoric acid. Blood agar with 5 % sodium chloride was used to isolate staphylococci from washes and milk. Species identification of pure cultures of staphylococci was performed using RapID Staph Plus kits. At the same time before and after milking the skin of teats with Udder Wash and Udder Forte reduces the incidence of Staphylococcus aureus to 4.1 ± 0.3 % of cases. Quantitative determination of staphylococci, including golden on the skin of the teats revealed a decrease in the latter from 283.5 ± 21.2 to 27.6 ± 2.1 CFU/cm3 flush after milking. It has been established that the number of staphylococci, including golden, on the skin of teats increases sharply under subclinical or clinical ointment. Thus, it was found that in the subclinical form of staphylococcal mastitis from the skin of teats isolated S. aureus in the amount of 790.6 ± 65.4 CFU/cm3 of wash. At the same time, in the clinical form of mastitis, the amount of S. aureus increased, on average to 8 thousand CFU/cm3 of flush. It was found that under the condition of sanitary treatment of milking equipment with alternate use of alkaline and acid detergents and disinfectants, only 25 % of washes were staphylococci, and gold – up to 5 % of samples. It was found that in 43.7 ± 3.9 % of raw milk samples Staphylococcus aureus was not detected in 1 cm3. The number of milk samples containing Staphylococcus aureus up to 100 CFU/cm3 was 39.1 ± 3.3 %, and 17.2 ± 1.4 % of milk samples were contaminated with Staphylococcus aureus in the amount of 101 to 200 CFU/cm3. Therefore, the presence of more than 200 CFU/cm3 in freshly milked Staphylococcus aureus in milk indicates the ineffectiveness of antimastitis measures, skin remediation of teats and sanitation of milking equipment.


Download data is not yet available.


Al-Ashmawy, M. A., Sallam, K. I., Abd-Elghany, S. M., Elhadidy, M., & Tamura, T. (2016). Prevalence, mo-lecular characterization, and antimic robial suscepti-bility of methicillin-resistant Staphylococcus aureus iso lated from milk and dairy products. Foodborne Pathogens and Disease, 13(3), 156–162. doi: 10.1089/fpd.2015.2038.

Berhilevych, O. М., Kasianchuk, V. V., Kukhtyn, M. D., Lotskin, I. М., Garkavenko, T. O., & Shubin, P.A. (2017). Characteristics of antibiotic sensitivity of Staphylococcus aureus isolated from dairy farms in Ukraine. Regulatory Mechanisms in Biosystems, 8(4), 559–563. doi: 10.15421/021786.

Carfora, V., Caprioli, A., Marri, N., Sagrafoli, D., Boselli, C., Giacinti, G., Sor bara L., Dottarelli S., Battisti A., & Amatiste, S. (2015). Enterotoxin genes, enterotoxin production, and methicillin resistance in Staphylococ-cus aureus isolated from milk and dairy products in Central Italy. International Dairy Journal, 42, 12–15. doi: 10.1016/j.idairyj.2014.10.009.

Cremonesi, P., Perez, G., Pisoni, G., Moroni, P., Morandi, S., Luzzana, M., Brasca, M., & Castiglioni, B. (2007). Detection of enterotoxigenic Staphylococcus aureus isolates in raw milk cheese. Letters in applied microbi-ology, 45(6), 586–591. doi: 10.1111/j.1472-765X.2007.02231.x.

Doulgeraki, A. I., Di Ciccio, P., Ianieri, A., & Nychas, G. J. E. (2017). Methicillin-resistant food-related Staphylo-coccus aureus: A review of current knowledge and biofilm formation for future studies and applications. Research in Microbiology, 168(1), 1–15. doi: 10.1016/j.resmic.2016.08.001.

Efimochkina, N. R., Kuvaeva, I. B., Fluer, F. S. (2011). Znachenie nekotoryih potentsialno patogennyih mikroorganizmov v vozniknovenii pischevyih toksikozov. Soobschenie 1. S. aureus i stafilokok-kovyie enterotoksinyi [The importance of some po-tentially pathogenic microorganisms in the occurrence of food toxicosis. Report 1.S. Aureus and staphylo-coccal enterotoxins]. Voprosyi pitaniya, 6 (in Russian).

Habib, F., Rind, R., Durani, N., Bhutto, A. L., Buriro, R. S., Tunio, A., Aijaz, N., Lakho, S. A., Bugti, A. G., & Shoaib, M. (2015). Morphological and cultural char-acterization of Staphylococcus aureus isolated from differ rent animal species. Journal of Applied Envi-ronmental and Biological Sciences, 5(2), 15–26.

Horiuk, Y., Kukhtyn, M., Perkiy, Y., & Horiuk, V. (2018). Distribution of main pathogens of mastitis in cows on dairy farms in the western region of Ukraine. Scientific Messenger of LNU of Veterinary Medicine and Bio-technologies. Series: Veterinary Sciences, 20(83), 115–119. doi: 10.15421/nvlvet8322.

Horiuk,Yu. V., Kukhtyn, M. D., Perkiy, Y. B., & Horiuk, V. V. (2018). Resistance of the mainpathogens of mastitis of cows to modern antimicrobial drugs. Sci-ence and Technology Bulletin of SRC for Biosafety and Environmental Control of AIC, 6(2), 49–53.

Horiuk, Yu., Kukhtyn, M., Kovalenko, V., Kornienko, L., Horiuk, & V., Liniichuk N. (2019). Biofilm formation in bovine mastitis pathogens and the effect on them of antimicrobial drugs. Independent journal of man-agement and production (IJM&P), 7(10), 897–910. doi: 10.14807/ijmp.v10i7.1012.

Jaber, S., Weder, D., Bridy, C., Huguenin, M. C., Robert, L., Hummerjohann, J., & Stephan, R. (2015). Out-break of staphylococcal food poisoning among chil-dren and staff at a Swiss boarding school due to soft cheese made from raw milk. Journal of Dairy Science, 98, 2944–2948. doi: 10.3168/jds.2014-9123.

Kasianchuk, V., Kryzhanivskyi, Ya., Danylenko, I., & Kukhtyn, M. (2003). Osnovni zakonomirnosti obsi-meninnia moloka zolotystym stafilokokom [The main patterns of contamination of milk with Staphy-lococcus aureus]. Veterynarna medytsyna Ukrainy, 10, 43–45 (in Ukrainian).

Kukhtyn, M. D., Horyuk, Y. V., Horyuk, V. V., Yaroshen-ko, T. Y., Vichko, O. I., & Pokotylo, O. S. (2017). Bio-type characterization of Staphylococcus aureus iso-lated from milk and dairy products of private produc-tion in the western regions of Ukraine. Regulatory Mechanisms in Biosystems, 8(3), 384–388. doi: 10.15421/021759.

Kukhtyn, M. D., Kovalenko, V. L., Pokotylo, O. S., Horyuk, Y. V., Horyuk, V. V., & Pokotylo, O. O. (2017) Staphylococcal contamination of raw milk and handmade dairy products, which are realized at the markets of Ukraine. Journal for Veterinary Medi-cine, Biotechnology and Biosafety, 3(1), 12–16. URL:

Macori, G., Bellio, A., Bianchi, D. M., Gallina, S., Adriano, D., Zuccon, F., Chiesa, F., Acutis, P. L., Casalinuovo, F., & Decastelli, L. (2016). Molecular typing of Staph-ylococcus aureus isolate responsible for staphylococ-cal poisoning incident in homemade food. Italian Journal of Food Safety, 5(2), 5736. doi: 10.4081/ijfs.2016.5736.

Noble, W. C. (1998). Skin bacteriology and the role of Staphylococcus aureus in infection. The British Jour-nal of Dermatology, 139(53),9–12. doi: 10.1046/j.1365-2133.1998.1390s3009.x.

Thaker, H. C., Brahmbhatt, M. N., Nayak, J. B., & Thak-er, H. C. (2013). Isolation and identification of Staph-ylococcus aureus from milk and milk products and their drug resistance patterns in Anand, Gujarat. Vet-erinary World, 6(1), 10–13. doi: 10.5455/vetworld.2013.10-13.

Vos, P., Garrity, G., Jones, D., Krieg, N.R., Ludwig, W., Rainey, F.A., & Whitman, W. (Eds.). (2011). Bergey's Manual of Systematic Bacteriology: Volume 3: The Firmicutes (Vol. 3). Springer Science & Business Me-dia.

Wang, S. C., Wu, C. M., Xia, S. C., Yong-Hua, Q. I., Xia, L. N., & Shen, J. Z. (2009). Distribution of superanti-genic toxin genes in Staphylococcus aureus isolates from milk samples of bovine subclinical mastitis cases in two major diary production regions of China. Veterinary Microbiology, 137(3), 276–281. doi: 10.1016/j.vetmic.2009.01.007

Abstract views: 130
PDF Downloads: 100
How to Cite
Kukhtyn, M., Horiuk, Y., Salata, V., Klymyk, V., Vorozhbit, N., & Rushchinskaya, T. (2021). Staphylococcus aureus of raw cow’s milk. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 23(102), 53-59.

Most read articles by the same author(s)