Dynamics of hematological parameters in rabbits for osteosubstitution by hydroxyapatite ceramics doped with germanium and in complex with blood coagulation activator


Keywords: erythrocytes, integral hematological indices, leukocytes, bone fractures, reparative osteogenesis, thrombo-cytes.

Abstract

Bone tissue has powerful regenerative properties, thanks to which, with stable fixation, quite large amounts of skeletal bone damage can be successfully repaired. However, in the case of fragmentary fractures, the use of osteosynthesis methods alone does not always ensure the optimal course of reparative regeneration, as its regenerative potential is lost. Therefore, there is a need to replace post-traumatic bone defects and stimulate reparative osteogenesis. For this purpose, doped or doped with various elements (Ge, Si, Zn, Ag, Cu) composite materials. The aim of the study was to investigate the dynamics of hematological parameters in rabbits for osteosubstitution by hydroxyapatite ceramics doped with germanium and in combination with a blood clotting activator. Model defects were formed in the radial diaphysis and femur metaphysis in rabbits with a 3 mm and 4.2 mm diameter drill bit, respectively. Anesthesia included acepromazine, thiopenate, and lidocaine infiltration anesthesia. Animals of the first experimental group (n = 12) were replaced by defects with granules of hydroxyapatite ceramics doped with germanium (HTGe), the second (n = 12) hydroxyapatite ceramics doped with germanium with blood coagulation activator (HTGe + a), the third (n = 12). hydroxyapatite α + β with active (α + β + a), and control granules of undoped ceramics (HT). On day 7, animals of all groups had minor post-traumatic erythrocytopenia and oligochromemia. There was also a gradual increase in the content of leukocytes in the blood with a peak on the 14th day, which in the group HTGe + a lasted until the 30th day. The increase in the number of leukocytes and their peak values occurred within the physiological norm and only approached its upper limit. In most groups there was a pronounced thrombocytosis during the first 14 days with normalization to the 30th day, but in the 2nd experimental group the number of platelets returned to normal only on the 60th day. Changes in most integral hematological indices, which reflect the relationship between blood cell populations, are characteristic of the inflammatory-resorptive phase of reparative osteogenesis. The dynamics of hematological parameters indicates the absence of a pronounced reaction of the body to the implantation of hydroxyapatite composite doped with germanium. The combination of hydroxyapatite ceramics doped with germanium with coagulation activator eliminates post-traumatic oligochromemia and erythrocytopenia and significantly increases the level of hematological integral indices, which indicates a more intensive course of inflammatory-resorptive clinical resorptive aparation phase.

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References

Abd el Rahman A. el Garawany, Othman A. Al Sagair, Ezzat S. el Daly, Kamal A. El Shaikh, Mahmoud H., El Gebaly, Ahmed A., Mousa (2005). Effect of bacterial endotoxin on some metabolites and enzymes in rats serum. Medical Journal of Islamic World Academy of Sciences, 15(2), 65–72. URL: https://jag.journalagent.com/ias/pdfs/IAS_15_2_65_72.pdf.

Bian, D., Zhou, W., Den, J., & Li, Y. (2017). Development of magnesium-based biodegradable metals with dietary trace element germanium as orthopaedic implant applications. Acta Biomaterialia, 64, 421–436. doi: 10.1016/j.actbio.2017.10.004.

Brusko, A. T., & Gajko, G. V. (2005). Funkcional'naya perestrojka kostej i ee klinicheskoe znachenie. Lugansk, Luganskij gosudarstvennyj medicinskij universitet (in Russian).

Burianov, O. A., & Protsenko, V. V. (2014). Porivnialna kharakterystyka hidroksyapatytu i biokompozitu – materialiv dlia zamishchennia defektu kistky pislia vydalennia pukhlyny. Litopys travmatolohii ta ortopedii, 1–2, 50–56. URL: http://nbuv.gov.ua/ UJRN/Lto_2014_1-2_13 (in Ukrainian).

Chemerovskyi, V. O. (2020). Renthenohrafichna, makromorfolohichna i hematolohichna otsinka hidroksyapatytnoi keramiky z riznymy fizyko-khimichnymy vlastyvostiamy. Naukovyi visnyk veterynarnoi medytsyny, 1, 140–152. doi: 10.33245/ 2310-4902-2020-154-1-140-152 (in Ukrainian).

Chemych, M. D. (2003). Vykorystannia intehratyvnykh pokaznykiv endohennoi intoksykatsii, rozrakhovanykh za dopomohoiu Mytsrosoft Ekhtsel, v otsintsi efektyvnosti terapii hostroho shyhelozu. Visnyk Sumskoho derzhavnoho universytetu. Seriia Medytsyna, 7(53), 84–89 (in Ukrainian).

Chen, H., Hayakawa, D., Emura, S., Ozawa, Y., Okumu-ra, T., & Shoumura, S. (2002). Effect of low or high dietary calcium on the morphology of therat femur. Nistol. Histopathol., 17(4), 1129–1135. doi: 10.14670/HH-17.1129.

Chistyakova, G. N., Gazieva, I. A., & Remizova, I. I. (2005). Ispol'zovanie integral'nyh gematologicheskih indeksov dlya ocenki stepeni autointoksikacii organizma pri oslozhnyonnoj gestozom beremennosti. Klinicheskaya laboratornaya diagnostika, 12, 34–37 (in Russian).

Diedukh, N. V., & Nikolchenko, O. A. (2009). Reheneratsiia kistky pry alimentarnomu osteoporozi (eksperymentalne doslidzhennia). Ortopedija, travmatologija i protezirovanie, 2, 34–40. doi: 10.15674/0030-59872009234-40 (in Ukrainian).

Dmitrijev, V. (2018). Features of Dogs Treatment at Fractures of Peripheral Skeleton. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 20(83), 279–281. doi: 10.15421/nvlvet8355.

Dmitrijev, V., & Khomyn, N. (2017). Frequency of occurrence and peculiarities of bones fracture of the peripheral skeleton in dogs. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 19(82), 180-183. URL: https://nvlvet.com.ua/index.php/journal/article/view/1361.

Dolaychuk, O. P., Fedoruk, R. S., Kovalchuk, I. I., & Kropyvka, S. I. (2015). Physiological and biochemical processes in the organisms of rats when feeding them with different amounts of germanium citrate. The Animal Biology, 17(2), 50–56. doi: 10.15407/animbiol17.02.050.

Elikov, A. V. (2011). Zavisimost' pokazatelej eritrocitov u bol'nyh s perelomami kostej goleni v zavisimosti ot sroka. Ortopediya i travmatologiya Rosii, 39, 53–58 (in Russian).

Fedoruk, R. S., Kovalchuk, I. I., Romaniv, L. I., & Khrabko, M. I. (2014). Vplyv tsytrativ hermaniiu ta selenu na vmist lipidiv i vazhkykh metaliv v orhanizmi medonosnykh bdzhil. Biolohiia tvaryn, 16(2), 141–149 (in Ukrainian).

Fujii, A., Kuboyama, N., Yamane, J., Nakao, S., & Furukawa, Y. (1993). Effect of organic germanium compound (Ge-132) on experimental osteoporosis in rats. General Pharmacology, 24(6), 1527–1532. doi: 10.1016/0306-3623(93)90447-6.

Gajko, G. V., & Brusko, A. T. (2013). Teoreticheskie aspekty fiziologicheskoj i reparativnoj regeneracii kostej s pozicij sistemnyh predstavlenij. ZHurnal NAMN Ukrainy, 19(4), 471–481 (in Russian).

Grin, V. K., Fistal, E. Y., & Speransky, I. I. (2006). Integral and other hematologic leukocyte counts as a criterion for assessing the severity of burn disease, its complications and treatment efficacy. Proceedings of the scientific-practical. Conference “Sepsis: the problem of diagnosis, therapy and prevention”, 77–78.

Gurin, A. N., Komlev, V. S., Fadeeva, I.V., & Petrakova, N. V. (2012). Sravnitel'noe issledovanie zameshcheniya defektov kostnoj tkani osteoplasticheskimi materialami na osnove α- i β-trikal'cijfosfata. Stomatologiya, 6, 16–21 (in Russian).

Khrabko, M., Fedoruk, R., & Dolaichuk, O. (2016). Fizioloho-biokhimichni protsesy v orhanizmi samyts F0 i samtsiv F1 shchuriv za umov vypoiuvannia yim “nanohermaniiu” tsytratu i tsytratu hermaniiu khimichno syntezovanoho. Visnyk Lvivskoho universytetu. Seriia biolohichna, 73, 226–234 (in Ukrainian).

Klinger, M. H. F., & Jelkmann, W. (2002). Role of Blood Platelets in Infection and Inflammation. Journal of interferon & cytokine research, 22, 913–922. doi: 10.1089/10799900260286623.

Komisarenko, S. V., Luhovskoi, E. V., Rublenko, M. V., Andriiets, V. H., Korolova, D. S., Chernyshenko, T. M., Hornytska, O. V., Platonova, T. M., Makohonenko, Ye. M., & Chernyshenko, V.O. (2015). Sposib oderzhannia autolohichnoho fibrynovoho heliu dlia stymuliatsii reheneratsii kistkovykh i miakykh tkanyn i znyzhennia intensyvnosti zapalnykh protsesiv. Patent na korysnu model №100467. Ukraina. MPK (2015. 01). A61R 19/00 A61R 31/00 (in Ukrainian).

Li, L., Ruan, T., Lyu, Y., & Wu, B. (2017). Advancesin Effect of Germanium or Germanium Compoundson Animals. Journal of Biosciences and Medicines, 5, 56–73. doi: 10.4236/jbm.2017.57006.

Long, Q. C., Zeng, G. X., & Zhao, X. L. (1996). Pharmacocinetics of germanium after po beta- carboxyethilgermanium sesquioxide in 24 Chinese volunteers. Zhongguo Yao Li Xue Bao, 17(5), 415–418. URL: https://pubmed.ncbi.nlm.nih.gov/9863162.

Lukevic, E. A., Gar, T. K., Ignatovich, L. M., Mironov, V. F. (1990). Biologicheskaya aktivnost' soedinenij germaniya. Riga: Znanie (in Russian).

Menchikov, L. G., & Ignatenko, M. A. (2012). Biologicheskaya aktivnost' organicheskih soedinenij germaniya (obzor). Himiko-farmacevticheskij zhur-nal, 46(11), 3–6 (in Russian).

O’Neill, E., Awale, G., Daneshm, L., Umerah, O., & Lo, K. W.-H. (2018). The roles of ions on bone regeneration. Drug discovery today, 23(4), 879–890. doi: 10.1016/j.drudis.2018.01.049.

Raznatovskaya, E. N. (2012). Integral'nye indeksy endogennoj intoksikacii u bol'nyh himiorezistentnym tuberkulezom legkih. Aktual'nі pitannya farm. і med. nauki ta praktiki, 2(9), 119–120 (in Russian).

Rublenko, M. V., Chemerovskyi, V. O., Vlasenko, V. M., & Ulianchych, N. V. (2018). Otsinka osteointehratsiinykh i osteoinduktyvnykh vlastyvostei keramiky, lehovanoi kremniiem, za modelnykh perelomiv stehnovoi kistky u kroliv. Naukovyi visnyk veterynarnoi medytsyny, 2, 44–53. doi: 10.33245/2310-4902-2018-144-2-44-53 (in Ukraini-an).

Rublenko, M. V., Semeniak, S. A., & Ulianchych, N. V. (2014). Dynamika biomarkeriv reparatyvnoho osteohenezu za umov zamishchennia kistkovykh defektiv. Naukovyi visnyk LNUVMBT im. S. Z. Hzhytskoho, 16(3), 287–294. URL: http://nbuv.gov.ua/ UJRN/nvlnu_2014_16_3%281%29__40 (in Ukraini-an).

Sakhanda, I. V. (2014). Preparaty Hermaniiu ta yikh zastosuvannia v medytsyni. Ukr. nauk.-med. molodizh. zhurnal, 4(84), 83–86 (in Ukrainian).

Schell, H., Duda, G. N., Peters, A., Tsitsilonis, S., Johnson, K. A., & Schmidt-Bleek, K. (2017). The haematoma and its role in bone healing. Journal of Experimental Orthopaedics, 4, 5. doi: 10.1186/s40634-017-0079-3.

Speranskij, I. I., Samojlenko, G. E., & Lobacheva, M. V. (2009). Obshchij analiz krovi – vse li ego vozmozhnosti ischerpany? Integral'nye indeksy intoksikacii kak kriterii ocenki tyazhesti techeniya endogennoj intoksikacii, ee oslozhnenij i effektivnosti provodimogo lecheniya. Zdorov'e Ukrainy, 6(19), 51–57 (in Russian).

Sturmer, K. M. (1996). Pathophysiology disrupted bone healing. Orthopaede, 25(5), 386–393. doi: 10.1007/s001320050039.

Todosiuk, T. P. (2020). Rentheno- ta makromorfolohichna otsinka reparatyvnoho osteohenezu za implantatsii hidroksyapatytnoho kompozytu, lehovanoho hermaniiem. Naukovyi visnyk BNAU, 2, 183–194 (in Ukrainian).

Trufanov, I. I., Mirenkov K. V., & Andriyas, I. A. (2014). Kostnaya tkan' – kompozicionnaya osnova oporno-dvigatel'nogo apparata. Litopys travmatolohii ta ortopedii, 1–2, 26–29 (in Russian).

Winkler, T., Sass, F. A., Duda, G. N., & Schmidt-Bleek, K. (2018). A review of biomaterials in bone defect healing, remaining shortcomings and future opportunities for bone tissue engineering. Bone Joint Res, 7(3), 232–243. doi: 10.1302/2046-3758.73.BJR-2017-0270.R1.

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Published
2021-06-19
How to Cite
Todosiuk, T., Rublenko, M., & Vlasenko, V. (2021). Dynamics of hematological parameters in rabbits for osteosubstitution by hydroxyapatite ceramics doped with germanium and in complex with blood coagulation activator. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 23(102), 78-86. https://doi.org/10.32718/nvlvet10212

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