Indicators of protein metabolism and intensity of lipid peroxide oxidation in chickens with different vegetative status


Keywords: autonomic nervous regulation, lipid peroxidation, protein metabolism, chickens

Abstract

The tone of the autonomic nervous system, protein metabolism and enzymes in Cobb-500 crossbred chickens aged 35 and 60 days were studied. The experiment involved 24 chickens, 8 in each group. In birds, the tone of the autonomic nervous system was determined by the method of variation pulsometry. The essence of the method is to record the effect of sympathetic and parasympathetic systems on heart rate in chickens. The effect was determined by recording one hundred consecutive cardio intervals and counting the time interval between each cardiocycle. Venous blood samples were taken from each individual at the age of 35 and 60 days to determine the indicators of protein metabolism (total protein, albumin, globulins) and lipid peroxidation depending on the tone of the autonomic nervous system. It was found that the antioxidant level in chickens with a predominance of the sympathetic division prevailed in all other groups regardless of the age of the bird. At 35 days of age, plasma concentrations of diene conjugates were significantly higher in normatonics and vagotonics (P ˂ 0.05). There was a higher level of activity of ketodienes and Schiff bases in chickens with a predominance of the sympathetic division of autonomic nervous regulation compared with normotonics and vagotonics (P ˂ 0.01). The content of total protein, albumin and globulins prevailed in normotonic hens and was the lowest in vagotonic hens at the age of 35 days. The two-month-old bird with a dominant parasympathetic division of the autonomic nervous system and normotonic hens had the highest levels of total protein, globulins, and conversely, sympathicotonic hens outnumbered the latter in albumin levels. Histidine content was highest in chickens with a balanced type of autonomic nervous system compared to sympathicotonics and vagotonics (P ˂ 0.05). The concentration of sulfur-containing amino acid methionine was highest in vagotonic chickens (P < 0.05) compared with sympathicotonics. Threonine was highest in sympathicotonics compared with other groups of animals and significantly outweighed vagotonics (P < 0.05). The level of lipid peroxidation products in 60-day-old sympathicotonic chickens was consistently higher in contrast to normotonics and vagotonics. At the same time there was the smallest increase in the activity of diene conjugates and ketodienes (P < 0.05) in chickens with a dominant sympathetic division of the autonomic nervous system, which indicates a slowdown in the accumulation of the final products of lipid peroxidation.

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Published
2021-06-19
How to Cite
Studenok, A., Shnurenko, E., Karpovskyi, V., Trokoz, V., & Gutyj, B. (2021). Indicators of protein metabolism and intensity of lipid peroxide oxidation in chickens with different vegetative status. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 23(102), 110-118. https://doi.org/10.32718/nvlvet10217