Indicators of protein metabolism and intensity of lipid peroxide oxidation in chickens with different vegetative status

Keywords: autonomic nervous regulation, lipid peroxidation, protein metabolism, chickens


The tone of the autonomic nervous system, protein metabolism and enzymes in Cobb-500 crossbred chickens aged 35 and 60 days were studied. The experiment involved 24 chickens, 8 in each group. In birds, the tone of the autonomic nervous system was determined by the method of variation pulsometry. The essence of the method is to record the effect of sympathetic and parasympathetic systems on heart rate in chickens. The effect was determined by recording one hundred consecutive cardio intervals and counting the time interval between each cardiocycle. Venous blood samples were taken from each individual at the age of 35 and 60 days to determine the indicators of protein metabolism (total protein, albumin, globulins) and lipid peroxidation depending on the tone of the autonomic nervous system. It was found that the antioxidant level in chickens with a predominance of the sympathetic division prevailed in all other groups regardless of the age of the bird. At 35 days of age, plasma concentrations of diene conjugates were significantly higher in normatonics and vagotonics (P ˂ 0.05). There was a higher level of activity of ketodienes and Schiff bases in chickens with a predominance of the sympathetic division of autonomic nervous regulation compared with normotonics and vagotonics (P ˂ 0.01). The content of total protein, albumin and globulins prevailed in normotonic hens and was the lowest in vagotonic hens at the age of 35 days. The two-month-old bird with a dominant parasympathetic division of the autonomic nervous system and normotonic hens had the highest levels of total protein, globulins, and conversely, sympathicotonic hens outnumbered the latter in albumin levels. Histidine content was highest in chickens with a balanced type of autonomic nervous system compared to sympathicotonics and vagotonics (P ˂ 0.05). The concentration of sulfur-containing amino acid methionine was highest in vagotonic chickens (P < 0.05) compared with sympathicotonics. Threonine was highest in sympathicotonics compared with other groups of animals and significantly outweighed vagotonics (P < 0.05). The level of lipid peroxidation products in 60-day-old sympathicotonic chickens was consistently higher in contrast to normotonics and vagotonics. At the same time there was the smallest increase in the activity of diene conjugates and ketodienes (P < 0.05) in chickens with a dominant sympathetic division of the autonomic nervous system, which indicates a slowdown in the accumulation of the final products of lipid peroxidation.


Download data is not yet available.


Brambilla, G., Civitareale, C., Ballerini, A. et al. (2002). Response to oxidative stress as a welfare parameterin swine. Redox Rep., 7(3), 159–163. doi: 10.1179/135100002125000406.

Castro, F. L. S., Kim, Y., Xu, H. et al. (2019). The effect of total sulfur amino acid levels on growth performance, egg quality, and bone metabolism in laying hens subjected to high environmental temperature. Poultry science, 98(10), 4982–4993. doi: 10.3382/ps/pez275.

Castro, F. L. S., Su, H., Choi, S. et al. (2019). L-Arginine supplementation enhances growth performance, lean muscle, and bone density but not fat in broiler chickens. Poultry science, 98(4), 1716–1722. doi: 10.3382/ps/pey504.

Conde-Sieira M., Capelli, V., Álvarez-Otero, R. et al. (2020). Hypothalamic AMPKα2 regulates liver energy metabolism in rainbow trout through vagal innervation. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 18(1), 122–134. doi: 10.1152/ajpregu.00264.2019.

Conde-Sieira, M., Capelli, V., Álvarez-Otero, R. et al. (2019). Differential Role of Hypothalamic AMPKα Isoforms in Fish: an Evolutive Perspective. Molecular Neurobiology, 56, 5051–5066. doi: 10.1007/s12035-018-1434-9.

Corbridge, D. E. C. (2013). Phosphorus: chemistry, bio-chemistry and technology. CRC press, 6th edition.

Crane L. J., & Miller, D. L. (1977). Plasma protein synthe-sis by isolated rat hepatocytes. Journal of cell biology, 72(1), 11–25. doi: 10.1083/jcb.72.1.11.

Danchuk, O. V., Broshkov, M. M., Karpovsky, V. I. et al. (2020). Types of Higher Nervous Activity in Pigs: Characteristics of Behavior and Effects of Technolog-ical Stress. Neurophysiology, 52, 358–366. doi: 10.1007/s11062-021-09892-7.

Danchuk, O. V., Karposvkii, V. I., Tomchuk, V. A. et al. (2020). Temperament in Cattle: A Method of Evaluation and Main Characteristics. Neurophysiology, 52(1), 73–79. doi: 10.1007/s11062-020-09853-6.

Danchuk, O. V., Karpovskyі, V. I., & Trokoz, V. O. (2018). Antioxidant-prooxidant status in organism of pigs with different types of higher nervous activity under stress. Scientific journal “Fiziologichnyi Zhurnal”, 64(4), 26–32, doi: 10.15407/fz64.04.026.

De Koning, T. J. (2013). Amino acid synthesis deficien-cies. Pediatric Neurology Part III, 40, 1775–1783. doi: 10.1016/b978-0-444-59565-2.00047-2.

Esposito, T., Lobaccaro, J. M., Esposito, M. G. et al. (2016). Effects of low-carbohydrate diet therapy in over weight subjects with autoimmune thyroiditis: possible synergism with ChREBP. DrugDes.Devel. Ther., 10, 2939–2946. doi: 10.2147/DDDT.S106440.

Garaeva, O. (2011). Sulfur-containing amino acids as markers of stress. Buletinul Academiei de Ştiinţe. Ştiinţele vieţii, 3(315), 50–62.

Gotovsky, D. G., Sobolev, D. T., & Gisko, V. N. (2018). Indicators of protein metabolism of replacement chickens when they are grown in conditions with various microbial air pollution. Veterinary Journal of Belarus, 2(9), 6–8.

Havenstein, G. B., Ferket, P. R., & Qureshi, M. A. (2003). Growth, livability, and feed conversion of 1957 versus 2001 broilers when fed representative 1957 and 2001 broiler diets. Poult. Sci, 82(10), 1500–1508. doi: 10.1093/ps/82.10.1500.

Kaneko, J. J., Harvey, J. W., Bruss, M. L. et. al. (2008). Clinical biochemistry of domestic animals. Academic press, 6th edition. doi: 10.1016/B978-0-12-370491-7.00006-4.

Khlybova, S. V., & Cirkin, V. I. (2006). Free L-histidine as one of the regulators of physiological processes. Vyatka medical bulletin, 3–4:, 44–48. URL:

Khuzhakhmetova, L. K., & Teply, D. L. (2016). Pharmacological correction of lipid peroxidation and peroxide hemolysis of red blood cells in mature rats during immobilization stress. Physiology, Natural Sciences, 2(55), 66–71.

Komarova, N. V., & Kamentsev, Ya. S. (2006). A practical guide to the use of capillary electrophoresis systems “Kapel”. ООО “Veda”, 212.

Kondrakhin, I. P., Arkhipov, A. V., & Levchenko, V. I. (2004). Methods of veterinary clinical laboratory diagnostics. Reference, 171–172.

Kuznyak, G., & Savchuk, L. (2017). Protein nutrition of poultry and its dependence on age. Agricultural science and education of Podillya, 1, 334–336.

Kyryliv, B. Ya., Ratych, I. B., Gunchak, A. V., & Fedo-rovych, E. I. (2015). Biological and metabolic features of different species of poultry. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Agricultural Sciences, 17(61), 71–80. URL:

Lushchak, V. I., Bagnyukova, T. V., & Luzhna, L. I. (2006). Indices of oxidative stress. 2. Lipid peroxides. Ukr. biochemistry magazine, 78(5), 113–119. URL:

Machida, N., & Aohagi, Y. (2001). Electrocardiography, heart rates, and heart weights of free living birds. J. Zoo Wild Med, 32(1), 47–54. doi: 10.1638/1042-7260(2001)032[0047:ehrahw];2.

Martínez, Y., Li, X., Liu, G. et al. (2017). The role of methionine on metabolism, oxidative stress, and diseases. Amino Acids, 49(12), 2091–2098. doi: 10.1007/s00726-017-2494-2.

Meerson, F. Z. (2001). Heart protection against ischemic lesions: the role of stress-limiting system sand stabilizing myocardial structure. Russian journal of cardiology, 5, 49–59. URL: view/2058?locale=en_US.

Messina G., Vicidomini C., Viggiano A. et al. (2012). Enhanced parasympathetic activity of sportive women is paradoxically associated to enhanced resting energy expenditure. Auton. Neurosci. Basic Clin, 169(2), 102–106. doi: 10.1016/j.autneu.2012.05.003.

Navarro, X. (2002). Physiology of the autonomic nervous system. Rev. Neurol., 35(6), 553–562. doi: 10.33588/rn.3506.2002013.

Opalovskaya, G. M. (2001). Circadian rhythms of autonomic parameters during mental and physical activity. Bulletin of experimental biology and medicine, 132(5), 1029–1033. doi: 10.1023/a:1017943903292.

Polumbryk, M., Ivanov, S., & Polumbryk, O. (2013). Antioxidants in food systems. Mechanism of action. Ukrainian journal of food science, 1, 15–40.

Püschel, G. P. (2004). Control of hepatocyte metabolism by sympathetic and parasympathetic hepatic nerves. The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology, 280(1), 854–867. doi: 10.1002/ar.a.20091.

Reddy, B. S., Reddy, P. A., & Venkatasivakumar, R. A. (2016). Study on electrocardiographic patterns in turkeys (Meleagris gallopavo). Inter J. Vet. Sci., 5(2), 79–82.

Stalnaya, I. D. (1977). Method for determination of diene conjugation unsaturated higher fatty acids. Modern methods in biochemistry, 63–64.

Studenok, A. A., Shnurenko, E. O., Trokoz, V. O., Karpovsky, V. I., Zhurenko, O. V., Krivoruchko, D. I. (2020). A method of assessing the tone of the autonomic nervous system in chickens. Ukraine Pa-tent 142943. inventors; Nat. Univ. Life and Environm. Sci. Ukraine, assignee (2020, Jul 10).

Sturkie, P. D. (1986). Avian Physiology 4thed. Springer, 167–191.

Thrall, M. A., Weiser, G., Allison, R. W. et al. (2012). Vet-erinary hematology and clinical chemistry. John Wiley & Sons, 2th edition.

Trenzado, C., Hidalgo, M. C., Garsia-Gallego, M. et al. (2005). Antioxidant enzymes and lipid peroxidation in sturgeon Acipenser naccarii and trout Oncorhynchus mykiss. A comparative study J. Aquaculture, 254(1-4), 758–767. doi: 10.1016/j.aquaculture.2005.11.020.

Tybinka, А. M. (2011). Features of variation-pulsometric indexes of chickens. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 13(4(50)), 446–449. URL: 13_4%281%29__84.

West, B. J. (2010). The wisdom of the body; a contemporary view. Front. Physiol., 1(1), 1–2, doi: 10.3389/fphys.2010.00001.

Zenkov, M. K., Lankin, V. Z., & Menshikova, E. B. (2001) Oxidative stress. Biochemical and pathophysiological aspects, Moscov, MAIK “Nau-ka/Interperiodika URL:

Abstract views: 87
PDF Downloads: 49
How to Cite
Studenok, A., Shnurenko, E., Karpovskyi, V., Trokoz, V., & Gutyj, B. (2021). Indicators of protein metabolism and intensity of lipid peroxide oxidation in chickens with different vegetative status. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 23(102), 110-118.

Most read articles by the same author(s)

<< < 1 2