The function of antimicrobial peptide MPX on the apoptosis and barrier of IPEC-J2 cells


  • Xueqin Zhao College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China https://orcid.org/0000-0002-6117-8537
Keywords: antimicrobial peptide MPX, Escherichia coli, IPEC-J2 cells

Abstract

The porcine intestinal epithelial cell line IPEC-J2 cells, which were isolated from neonatal piglet mid-jejunum, the main components of the intestinal epithelium and play an important role in the intestine. Escherichia coli is an important cause of diarrhea in human and animal. E.coli infections are the leading cause of travelers' diarrhea and a major cause of diarrhea in developing nations, where it can be life-threatening among children. The aim of this study is to explore MPX against E. coli to inhibit IPEC-J2 cells apoptosis and enhance cell barrier. In this study, scanning electron microscopy results found that E. coli infection caused cell apoptosis and destroyed cell membranes of IPEC-J2 cell. MPX effectively alleviated apoptosis of IPEC-J2 cells. The laser confocal results further found that MPX prevented cell apoptosis by inhibiting caspase-3 and caspase-9 activation. In addition, it was found that MPX regulated the expression of tight junction proteins ZO-1, Occludin, and Claudin-1 in IPEC-J2 cells and is closely related to Rac1 by adding a Rac1 inhibitor. The results of adhesion and invasion experiments further found that MPX inhibited bacterial adhesion and invasion through Rac1. The above results indicated that MPX has better function in inhibiting IPEC-J2 cells apoptosis and enhancing cell barrier.

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Abstract views: 16
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Published
2021-06-19
How to Cite
Zhao, X. (2021). The function of antimicrobial peptide MPX on the apoptosis and barrier of IPEC-J2 cells. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 23(102), 125-129. https://doi.org/10.32718/nvlvet10219