Autonomous regulation of antioxidant protection and protein exchange in chickens


  • E. O. Shnurenko National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine https://orcid.org/0000-0002-1121-4106
  • A. A. Studenok National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine https://orcid.org/0000-0002-0740-3609
  • V. I. Karpovskyi National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine https://orcid.org/0000-0003-3858-0111
  • V. O. Trokoz National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine https://orcid.org/0000-0001-8619-195X
  • B. V. Gutyj Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, Ukraine https://orcid.org/0000-0002-5971-8776
  • A. Y. Torzhash National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
  • V. F. Radchikov Scientific and Practical Center of the National Academy of Sciences Belarus on animal husbandry, Zhodino, Belarus https://orcid.org/0000-0003-4090-6635
Keywords: autonomous regulation, antioxidant system, lipid peroxidation, protein metabolism, chickens

Abstract

The tone of the autonomic nervous system, protein metabolism and the activity of the antioxidant system in Cobb-500 cross chickens aged 35 and 60 days were researched. The experiment involved 24 chickens, 8 in each group. In birds, the tone of the autonomic nervous system was determined by the method of variation pulsometry by recording the influence of the sympathetic and parasympathetic systems on the heart rate in chickens. The effect was determined by counting one hundred consecutive cardio intervals and the time interval between each cardiocycle. Venous blood samples were taken from each individual at the age of 35 and 60 days to determine protein metabolism (total protein, albumin, globulins), enzymatic and non-enzymatic links of the antioxidant system and lipid peroxidation depending on the tone of the autonomic nervous system. It was found that the antioxidant level in chickens with a predominance of the sympathetic division prevailed in all other groups regardless of the age of the bird. At 35 days of age, plasma concentrations of diene conjugates were significantly higher in normatonics and vagotonics (P ˂ 0.05). There was a higher level of activity of ketodienes and Schiff bases in chickens with a predominance of the sympathetic division of autonomic nervous regulation compared with normotonics and vagotonics (P ˂ 0.01). The content of total protein, albumin and globulins prevailed in normotonic hens and was the lowest in vagotonic hens at the age of 35 days. The two-month-old bird with a dominant parasympathetic division of the autonomic nervous system and normotonic hens had the highest levels of total protein, globulins, and conversely, sympathicotonic hens outnumbered the latter in albumin levels. Histidine content was highest in chickens with a balanced type of autonomic nervous system compared to sympathicotonics and vagotonics (P ˂ 0.05). The concentration of sulfur-containing amino acid methionine was highest in vagotonic chickens (P < 0.05) compared with sympathicotonics. Threonine was highest in sympathicotonics compared with other groups of animals and significantly outweighed vagotonics (P < 0.05). The level of lipid peroxidation products in 60-day-old sympathicotonic chickens was consistently higher in contrast to normotonics and vagotonics. At the same time there was the smallest increase in the activity of diene conjugates and ketodienes (P < 0.05) in chickens with a dominant sympathetic division of the autonomic nervous system, which indicates a slowdown in the accumulation of the final products of lipid peroxidation. It was found that the predominance of sympathetic tone is characterized by low activity of enzymes of the antioxidant system and tocopherol (P < 0.001, P < 0.01) at 35 and 60 days of age. Indicators of the activity of the antioxidant system had the highest level in vagotonic chickens of the studied age period (P < 0.05, P < 0.01, P < 0.001). The relationship between catalase and tocopherol levels in vagotonic chickens at 35 days of age, which increases at 60 days of age r = 0.53 (P < 0.01) was found.

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References

Castro, F. L. S., Choi H. Su S., Koo, E., & Kim, W. K. (2019a). L-Arginine supplementation enhances growth performance, lean muscle, and bone density but not fat in broiler chickens. Poultry science, 98(4), 1716–1722. doi: 10.3382/ps/pey504.

Castro, F. L. S., Kim, Y., Xu, H. et al. (2019b). The effect of total sulfur amino acid levels on growth perfor-mance, egg quality, and bone metabolism in laying hens subjected to high environmental temperature. Poultry science, 98(10), 4982–4993. doi: 10.3382/ps/pez275.

Conde-Sieira M., Capelli V., Álvarez-Otero R. et al. (2020) Hypothalamic AMPKα2 regulates liver energy metabolism in rainbow trout through vagal innerva-tion. American Journal of Physiology-Regulatory, In-tegrative and Comparative Physiology, 18(1), 122–134. doi: 10.1152/ajpregu.00264.2019.

Corbridge, D. E. C. (2013). Phosphorus: chemistry, bio-chemistry and technology. CRC press, 6th edition.

Danchuk, O. V., Broshkov, M. M., Karpovsky, V. I. et al. (2020b). Types of Higher Nervous Activity in Pigs: Characteristics of Behavior and Effects of Technolog-ical Stress. Neurophysiology, 52(5), 358–366. doi: 10.1007/s11062-021-09892-7.

Danchuk, O. V., Karposvkii, V. I., Tomchuk, V. A. et al. (2020a). Temperament in Cattle: A Method of Evaluation and Main Characteristics. Neurophysiology, 52(1), 73–79. doi: 10.1007/s11062-020-09853-6.

Danchuk, O. V., Karpovskyі, V. I., & Trokoz, V. O. (2018). Antioxidant-prooxidant status in organism of pigs with different types of higher nervous activity under stress. Scientific journal “Fiziologichnyi Zhur-nal,” 64(4), 26–32. doi: 10.15407/fz64.04.026.

De Koning, T. J. (2013). Amino acid synthesis deficien-cies. Pediatric Neurology, 113, 1775–1783. doi: 10.1016/b978-0-444-59565-2.00047-2.

Dubinina, E. E., Salnikova, L. Ya., & Efimova, L. F. (1983). Aktivnost i izofermentnyiy spektr superoksid-dismutazyi eritrotsitov i plazmyi krovi cheloveka. Lab. Delo, 10, 30–33 (in Russian).

Esposito, T., Lobaccaro, J. M., Esposito, M. G. et al. (2016). Effects of low-carbohydrate diet therapy in over weight subjects with autoimmune thyroiditis: possible synergism with ChREBP. Drug Des. Devel. Ther, 10, 2939–2946. doi: 10.2147/DDDT.S106440.

Garaeva, O. (2011). Sulfur-containing amino acids as markers of stress. Buletinul Academiei de Ştiinţe. Şti-inţele vieţii, 3(315), 50–62.

Gotovsky, D. G., Sobolev, D. T., & Gisko, V. N. (2018). Indicators of protein metabolism of replacement chickens when they are grown in conditions with vari-ous microbial air pollution. Veterinary Journal of Bel-arus, 2(9), 6–8.

Huzhahmetova, L. K., & Sentyurova, L. G. (2015). Dinamika protsessov perekisnogo okisleniya lipidov u kryis pri stresse i posle farmakologicheskoy korrektsii. Sovremennyie problemyi nauki i obrazovaniya, 4. URL: https://science-education.ru/ru/article/view?id= 21144 (in Russian).

Kaneko, J. J., Harvey, J. W., Bruss, M. L. et. al. (2008). Clinical biochemistry of domestic animals. Academic press, 6th edition. URL: https://www.elsevier.com/books/clinical-biochemistry-of-domestic-animals/kaneko/978-0-12-370491-7.

Khlybova, S. V., & Cirkin, V. I. (2006). Free L-histidine as one of the regulators of physiological processes. Vyatka medical bulletin, 3-4, 44–48.

Komarova, N. V., & Kamentsev, Ya. S. (2006). A practi-cal guide to the use of capillary electrophoresis sys-tems “Kapel”. ООО “Veda”.

Kondrakhin, I. P., Arkhipov, A. V., & Levchenko, V. I. (2004). Methods of veterinary clinical laboratory di-agnostics. Reference. M.: KolosS.

Korolyuk, M. A., Ivanova, A. I., & Mayorova, I. T. (1988). Metod opredeleniya aktivnosti katalazyi. La-boratornoe delo, 1, 16–19 (in Russian).

Kuznyak, G., & Savchuk, L. (2017). Protein nutrition of poultry and its dependence on age. Agricultural sci-ence and education of Podillya, 1, 334–336. URL: http://sophus.at.ua/Conf_2017/Zb_PDATU_03_2017_p1.pdf.

Lushchak, V. I., Bagnyukova, T. V., & Luzhna, L. I. (2006). Indices of oxidative stress. 2. Lipid peroxides. Ukr. biochemistry magazine, 78(5), 113–119. URL: https://pubmed.ncbi.nlm.nih.gov/17494327.

Machida, N., & Aohagi, Y. (2001). Electrocardiography, heart rates, and heart weights of free living birds. J. Zoo Wild Med, 32(1), 47–54. doi: 10.1638/1042-7260(2001)032[0047:ehrahw]2.0.co;2.

Martínez, Y., Li, X., Liu, G. et al. (2017). The role of methionine on metabolism, oxidative stress, and dis-eases. Amino Acids, 49(12), 2091–2098. doi: 10.1007/s00726-017-2494-2.

Meerson, F. Z. (2001). Heart protection against ischemic lesions: the role of stress-limiting system sand stabi-lizing myocardial structure. Russian journal of cardi-ology, 5, 49–59. URL: https://russjcardiol.elpub.ru/ jour/article/view/2058?locale=en_US.

Messina, G., Vicidomini, C., Viggiano, A. et al. (2012). Enhanced parasympathetic activity of sportive women is paradoxically associated to enhanced resting energy expenditure. Auton. Neurosci. Basic Clin, 169(2), 102–106. doi: 10.1016/j.autneu.2012.05.003.

Opalovskaya, M. (2001). Circadian rhythms of autonomic parameters during mental and physical activity. Bulletin of experimental biology and medicine, 132(5), 1029–1033. doi: 10.1023/a:1017943903292.

Patreva, L. S. (2018). Tehnologiya vyrobnytstva produk-tsiyi ptahivnitstva: kurs lektsiy. Mykolajiv, MNAU (in Ukrainian).

Püschel, G. P. (2004). Control of hepatocyte metabolism by sympathetic and parasympathetic hepatic nerves. The Anatomical Record Part A: Discoveries in Mo-lecular, Cellular, and Evolutionary Biology, 280(1), 854–867. doi: 10.1002/ar.a.20091.

Reddy, B. S., Reddy, P. A., & Venkatasivakumar, R. A (2016). Study on electrocardiographic patterns in tur-keys (Meleagris gallopavo). Inter J. Vet. Sci., 5(2), 79–82. URL: http://www.ijvets.com/pdf-files/Volume-5-no-2-2016/79-82.pdf.

Shnurenko, E. O., Studenok, A. A., Gutyj, B. V., Karpov-skiy, V. I., & Trokoz, V. O. (2021). Age features of the interrelation between catalase and tocopherol activity in chickens with different types of autonomous nervous regulation. Colloquium-journal, 12(99), 12–15. URL: https://journals.indexcopernicus.com/api/ file/viewByFileId/1219406.pdf.

Stalnaya, I. D. (1977). Method for determination of diene conjugation unsaturated higher fatty acids. Modern methods in biochemistry, 63–64. URL: https://chem21.info/page/024222249026222064160077114027067029203041198200.

Studenok, A. A., Shnurenko, E.O., Trokoz, V. O., Kar-povsky, V. I., Zhurenko, O. V., & Krivoruchko, D. I. (2020). A method of assessing the tone of the auto-nomic nervous system in chickens. Patent № 142943. Ukraine. u201910996.

Thrall, M. A., Weiser, G., Allison, R.W. et al. (2012). Veterinary hematology and clinical chemistry. John Wiley & Sons, 2th edition.

Trenzado, C., Hidalgo, M. C., Garsia-Gallego, M. et al. (2005). Antioxidant enzymes and lipid peroxidation in sturgeon Acipenser naccarii and trout Oncorhynchus mykiss, A comparative study. J. Aquaculture, 254(1-4), 758–767. doi: 10.1007/s10695-017-0356-1.

Tybinka, А. M. (2011). Features of variation-pulsometric indexes of chickens. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 13(4(50)), 446–449. URL: http://nbuv.gov.ua/UJRN/nvlnu _2011_13_4%281%29__84.

Vasyliv, A. P. (2017). The role of types of higher nervous activity in protein metabolism in pigs. Author's ref. dis. for science. degree cand. vet. science: special. 03.00.13 – Human and animal physiology. URL: http://dglib.nubip.edu.ua:8080/jspui/bitstream/123456789/4250/1/Vasyliv%20A.P.pdf.

Venditti P, & Meo, S. D. (2006). Thyroid hormone-induced oxidative stress. Cell Mol Life Sci, 63(4), 414–434. doi: 10.1007/s00018-005-5457-9.

Vlislo, V. V. (2012). Laboratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarniy medytsyni [Laboratory methods of research in biology, animal husbandry and veterinary medicine]. Spolom, Lviv (in Ukrainian).

Zenkov, M. K., Lankin, V. Z., & Menshikova, E. B. (2001). Oxidative stress. Biochemical and pathophysiological aspects, Moscov, MAIK “Nauka/Interperiodika.

Сrane, L. J., & Miller D. L. (1977). Plasma protein syn-thesis by isolated rat hepatocytes. Journal of cell biol-ogy, 72(1), 11–25. doi: 10.1083/jcb.72.1.11.

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Published
2021-11-27
How to Cite
Shnurenko, E., Studenok, A., Karpovskyi, V., Trokoz, V., Gutyj, B., Torzhash, A., & Radchikov, V. (2021). Autonomous regulation of antioxidant protection and protein exchange in chickens. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 23(103), 43-50. https://doi.org/10.32718/nvlvet10307

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