Therapeutic efficacy of bacteriophage drug Fagomast in clinical mastitis of cows

Keywords: clinical mastitis, cows, therapeutic efficacy, Fagomast


Phage therapy is an effective and safe way to treat infectious diseases caused by various types of bacteria, including mastitis in cows. However, the number of in vivo studies on phages to treat cattle with staphylococcal mastitis is relatively low. This study aimed to evaluate the therapeutic efficacy of the bacteriophage drug Fagomast in treating cows with staphylococcal mastitis in its clinical manifestation and compare the results with commercial antimastitis drugs based on antibiotics. For this purpose, groups of cows with signs of clinical mastitis caused by S. aureus were formed. Cows of the experimental group were administered intracisternal bacteriophage drug Fagomast, and animals of the control group were used drugs based on antibiotics. Cows were considered healthy in the disappearance of clinical manifestations of the disease and adverse reactions in mastidin test. According to the research results, it is established that the effectiveness of therapy in the clinical form of staphylococcal mastitis with the use of intra-tank antimastitis drugs based on antibiotics is from 66.7 to 100 %, and with the help of bacteriophage drug Fagomast – 71.4 %. The period during which the rejection of milk during the treatment of clinical mastitis drug Fagomast was 1.5 times less than in treating such mastitis drugs containing antibiotics. Thus, the drug Fagomast is generally not inferior to traditional treatment methods with antibiotics. Therefore, the use of Fagomast in treating staphylococcal mastitis is relevant and promising.


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Barbu, E. M., Cady, K. C., & Hubby, B. (2016). Phage therapy in the era of synthetic biology. Cold Spring Harbor perspectives in biology, 8(10), a023879. DOI: 10.1101/cshperspect.a023879.

Breyne, K., Honaker, R. W., Hobbs, Z., Richter, M., Żaczek, M., Spangler, T., ... & Mokres, L. (2017). Effi-cacy and safety of a bovine-associated Staphylococ-cus aureus phage cocktail in a murine model of masti-tis. Frontiers in microbiology, 8, 2348. DOI: 10.3389/fmicb.2017.02348.

Capparelli, R., Parlato, M., Borriello, G., Salvatore, P., & Iannelli, D. (2007). Experimental phage therapy against Staphylococcus aureus in mice. Antimicrobial agents and chemotherapy, 51(8), 2765–2773. DOI: 10.1128/AAC.01513-06.

Chan, B. K., Abedon, S. T., & Loc-Carrillo, C. (2013). Phage cocktails and the future of phage therapy. Fu-ture microbiology, 8(6), 769–783. DOI: 10.2217/fmb.13.47.

Geng, H., Zou, W., Zhang, M., Xu, L., Liu, F., Li, X., Wang, L., & Xu, Y. (2020). Evaluation of phage ther-apy in the treatment of Staphylococcus aureus-induced mastitis in mice. Folia Microbiol. 65, 339–351. DOI: 10.1007/s12223-019-00729-9.

Gomes, F., & Henriques, M. (2016). Control of bovine mastitis: old and recent therapeutic approaches. Cur-rent microbiology, 72(4), 377–382. DOI: 10.1007/s00284-015-0958-8.

Górski, A., Jończyk-Matysiak, E., Łusiak-Szelachowska, M., Międzybrodzki, R., Weber-Dąbrowska, B., & Bo-rysowski, J. (2017). The potential of phage therapy in sepsis. Frontiers in immunology, 8, 1783. DOI: 10.3389/fimmu.2017.01783.

Horiuk, Y. V., Havrylianchyk, R. Y., Horiuk, V. V., Ku-khtyn, M. D., Stravskyy, Y. S., & Fotina, H. A. (2018a). Comparison of the minimum bactericidal concentration of antibiotics on planktonic and biofilm forms of Staphylococcus aureus: Mastitis causative agents. Research Journal of Pharmaceutical, Biologi-cal and Chemical Sciences, 9(6), 616–622.

Horiuk, Y. V., Kukhtyn, M. D., Perkiy, Y. B., & Horiuk, V. V. (2018b). Distribution of main pathogens of mastitis in cows on dairy farms in the western region of Ukraine. Scientific Messenger of Lviv National Uni-versity of Veterinary Medicine and Biotechnologies, 20(83), 115–119. DOI: 10.15421/nvlvet8322.

Horiuk, Y., Kukhtyn, M., Salata, V., & Horiuk, V. (2020). Species composition and methicillin resistance of staphylococci taken on dairy farms. Scientific Mes-senger of LNU of Veterinary Medicine and Biotech-nologies. Series: Veterinary Sciences, 22(97), 13–19. DOI: 10.32718/nvlvet9703.

Iwano, H., Inoue, Y., Takasago, T., Kobayashi, H., Fu-rusawa, T., Taniguchi, K., ... & Tamura, Y. (2018). Bacteriophage ΦSA012 has a broad host range against Staphylococcus aureus and effective lytic ca-pacity in a mouse mastitis model. Biology, 7(1), 1–13. DOI: 10.3390/biology7010008.

Kukhtyn, M. D., Kovalenko, V. L., Horyuk, Y. V., Horyuk, V. V., & Stravskyy, Y. S. (2016). Bacterial biofilms formation of Cattle mastitis pathogens. Jour-nal for veterinary medicine, biotechnology and bi-osafety, 2(4), 30–32.

Kurtyak, B. M., Boyko, P. K., Boyko, O. P., Sobko, G. V., Romanovych, M. S., Pundyak, T. O., Mandygra, Yu. M., & Gutyj, B. V. (2021). Autogenous vaccines are an effective means of controlling the epizootic pro-cess of mastitis in cows. Ukrainian Journal of Ecology, 11 (3), 145–152. doi: 10.15421/2021_157.

Moelling, K., Broecker, F., & Willy, C. (2018). A wake-up call: we need phage therapy now. Viruses, 10(12), 688. DOI: 10.3390/v10120688.

Sharun, K., Dhama, K., Tiwari, R., Gugjoo, M. B., Iqbal Yatoo, M., Patel, S. K., & Chaicumpa, W. (2021). Ad-vances in therapeutic and managemental approaches of bovine mastitis: a comprehensive review. Veteri-nary Quarterly, 41(1), 107–136. DOI: 10.1080/01652176.2021.1882713.

Silva, S. R., Araujo, J. P., Guedes, C., Silva, F., Almeida, M., & Cerqueira, J. L. (2021). Precision technologies to address dairy cattle welfare: focus on lameness, mas-titis and body condition. Animals, 11(8), 2253. DOI: 10.3390/ani11082253.

Simmons, M., Drescher, K., Nadell, C. D., & Bucci, V. (2018). Phage mobility is a core determinant of phage–bacteria coexistence in biofilms. The ISME journal, 12(2), 531–543. DOI: 10.1038/ismej.2017.190.

Steele, N., & McDougall, S. (2014). Effect of prolonged duration therapy of subclinical mastitis in lactating dairy cows using penethamate hydriodide. N. Z. Vet. J., 62, 38–46. DOI: 10.1080/00480169.2013.830350.

Tashakkori, N., Khoramian, B., Moghadam, M. F., Hei-darpour, M., Mashayekhi, K., & Farzaneh, N. (2020). Evaluating the effectiveness of two bovine mastitis vaccines and their influences on oxidant and antioxi-dant capacities of milk. Tropical animal health and production, 52(3), 1493–1501. DOI: 10.1007/s11250-019-02156-x.

Teng, F., Xiong, X., Zhang, S., Li, G., Wang, R., Zhang, L., ... & Qiao, X. (2022). Efficacy Assessment of Phage Therapy in Treating Staphylococcus aureus-Induced Mastitis in Mice. Viruses, 14(3), 620. DOI: 10.3390/v14030620.

Titze, I., Lehnherr, T., Lehnherr, H., & Krömker, V. (2020). Efficacy of bacteriophages against Staphylo-coccus aureus isolates from bovine mastitis. Pharma-ceuticals, 13(3), 35. DOI: 10.3390/ph13030035.

Zhang, B., Yu, P., Wang, Z., & Alvarez, P. J. (2020). Hor-metic promotion of biofilm growth by polyvalent bac-teriophages at low concentrations. Environmental Sci-ence & Technology, 54(19), 12358–12365. DOI: 10.1021/acs.est.0c03558

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Horiuk, Y. (2022). Therapeutic efficacy of bacteriophage drug Fagomast in clinical mastitis of cows. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 24(105), 89-93.