Pathomorphological changes of the stomach and intestinum crassum in the intestinal form of parvoviral enteritis in dogs


Keywords: dogs, parvovirus enteritis, pathological autopsy, macroscopic changes, histological and histochemical studies pathomorphological diagnosis

Abstract

Based on the analysis of literature sources, monitoring results and our own research, it has been established that viral enteritis occupies a leading place in the infectious pathology of dogs and causes significant damage to animal owners. Viral enteritis, most commonly caused by parvovirus, causes severe gastrointestinal disorders and is one of the most common causes of infectious diarrhea in dogs under 6 months of age. Therefore, given the relevance of this issue, the result of our study is to clarify, supplement and summarize data on the pathomorphological features of the intestinal form of parvovirus infection in dogs. Diagnostic studies to confirm parvovirus enteritis were performed using VetExpert CPV Ag or CPV/CCV Ag rapid tests, as well as by ELISA and PCR. Dissection of dead animals was performed in the conventional sequence. For pathomorphological examination, samples were taken from the gastrointestinal tract, fixed in 10 % neutral formalin solution, followed by dehydration in alcohols of increasing concentration and the procedure of organ compaction according to the generally accepted method. Histological sections 6–10 μm thick were obtained using a microtome. The article presents the results of pathological changes in dogs with parvovirus enteritis, namely macroscopically under the serous membrane of the stomach revealed striped hemorrhages, duodeno-gastric reflux, manifested by gray-green color of the mucous membrane (bile accumulation). Histological methods have shown that the blood vessels of the submucosal base of the mucous membrane are clearly dilated, filled with blood. The mucous membrane is swollen, unevenly colored in all parts of the stomach. In the muscular membrane of the stomach wall, the vast majority of cells were in a state of granular dystrophy, and in some animals, necrotic changes in smooth muscle tissue were recorded. Histochemical studies revealed the development of microscopic changes that indicated hypersecretion of mucus (acidic proteins) in the stomach of sick dogs. No specific macroscopic changes in the colon were recorded. Microscopically, a small amount of cellular detritus with mucus fragments, goblet cell hyperplasia, and a reaction of epithelial cells that were in a state of granular, less often, hydropic dystrophy were recorded on the lumen of the colon on the mucosal surface.

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References

Castro, T., Cassia, R., Cubel, G. N., Gonçalves, L. P. S., Costa, E. M., Marcello, G. C. G., Labarthe, V. N., & Mendes-de-Almeida, F. (2013). Clinical, hematologi-cal and biochemical findings in puppies with corona-virus and parvovirus enteritis. Canadian Veterinary Journal, 54(9), 885–893. URL: https://www.ncbi.nlm.nih.gov/ pmc/articles/PMC3743577.

D’Oliveira, P.S.B., Cargnelutti, J.F., Masuda, E.K., Fighera, R.A., Kommers, G.D., Da’Silva, M.C., Weiblen, R. & Flores, E.F. (2018). Epidemiological, clinical and pathological features of canine parvovirus 2c infec-tion in dogs from southern Brazil. Pesquisa Veterinária Brasileira, 38(1), 113–118. DOI: 10.1590/ 1678-5150-pvb-5122.

Decaro, N., & Buonavoglia, C. (2012). Canine parvovi-rus-a review of epidemiological and diagnostic as-pects, with emphasis on type 2c. Veterinary Microbi-ology, 155(1), 1–12. DOI: 10.1016/j.vetmic.2011.09.007.

Decaro, N., Desario, C., Billi, M., Lorusso, E., Colaianni, M. L., Colao, V. & Buonavoglia, C. (2013). Evalua-tion of an in-clinic assay for the diagnosis of canine parvovirus. Veterinary Journal, 198(2), 504–507. DOI: 10.1016/j.tvjl.2013.08.032.

Filipov, C., Decaro, N., Desario, C., Amorisco, F., Sciarretta, R. & Buonavoglia C. (2011). Canine parvovirus epidemilogy in Bulgaria. Journal of Veterinary Diagnostic Investigation, 23(1), 152–154. DOI: 10.1177/104063871102300129.

Geetha, M. (2015). Epidemiology, pathogenesis, clinical findings and diagnosis of canine parvo viral infection – a mini review. International Journal of Scientific Engineering and Applied Science, 1(9), 21–27. URL: https://ijseas.com/volume1/v1i9/ijseas20150903.pdf.

Horalskyi, L. P., Khomych, V. T., & Kononskyi, O. I. (2019). Osnovy histolohichnoi tekhniky i mor-fofunktsionalni metody doslidzhennia u normi ta pry patolohii [Fundamentals of histological technique and morphofunctional research methods in normal and pathological conditions] Polissia, Zhytomyr (in Ukrainian).

Khatri, R., Mohan, H., Prasad, M., & Pundir, C.S. (2017). Epidemiology, Pathogenesis, Diagnosis and Treatment of Canine Parvovirus Disease in Dogs: A Mini Review. Journal of Veterinary Science & Medical Diagnosis, 6(3), 178–202. DOI: 10.4172/2325-9590.1000233.

Larson, L. J., & Schultz, R. D. (2007). Three-Year Serologic Immunity against Canine Parvovirus Type2 and Canine Adenovirus Type2 in Dogs Vaccinated with a Canine Combination Vaccine. Veterinary Therapeutics, 8(4), 305–310. URL: https://pubmed.ncbi.nlm.nih.gov/ 18183549.

Ling, M., Norris, J.M., Kelman, M., & Ward, M. P. (2012). Risk factors for death from canine parvoviral-related disease in Australia. Veterinary microbiology, 158(3–4), 280–290. DOI: 10.1016/j.vetmic.2012.02.034.

Lisova, V. V., & Radzykhovsʹkyy, M. L. (2018). Patomorfolohichna diahnostyka enterytiv virusnoyi etiolohiyi u sobak [Pathomorphological diagnosis of enteritis of viral etiology in dogs] Naukovyy visnyk LNUVM ta BT im. S.Z. Gzhytsʹkoho, 20(83), 299–303. DOI: 10.15421/nvlvet8360.

Marcovich, J. E., Stucker, K. M., Carr, A. H., Harbison, C. E., Scarlett, J. M., & Parrish, C. R. (2012). Effects of canine parvovirus strain variations on diagnostic test results and clinical management of enteritis in dogs. Journal of the American Veterinary Medical Associa-tion, 241(1), 66–72. DOI: 10.2460/javma.241.1.66.

McClure, V., Schoor, M., Thompson, P. N., Kjelgaard-Hansen, M., & Goddard, A. (2013). Evaluation of the use of serum C-reactive protein concentration to pre-dict outcome in puppies infected with canine parvovi-rus. Journal of the American Veterinary Medical As-sociation, 243(3), 361–366. DOI: 10.2460/ javma.243.3.361.

Mylonakis, M., Kalli, I., & Rallis, T. (2016). Canine par-voviral enteritis: an update on the clinical diagnosis, treatment, and prevention. Journals Veterinary Medi-cine, 7, 91–100. DOI: 10.2147/VMRR.S80971.

Netherton, C. L., & Wileman, T. (2011). Virus factories, double membrane vesicles and viroplasm generated in animal cells. Current opinion in virology, 1(5), 381–387. DOI: 10.1016/j.coviro.2011.09.008.

Parker, J., Murphy, M., Hueffer, K., & Chen, J. (2017). Investigation of a Canine Parvovirus Outbreak using Next Generation Sequencing. Scientific Reports, 7, 9633. DOI: 10.1038/s41598-017-10254-9.

Perez, R., Calleros, L., Marandino, A., Sarute, N., Iraola, G., Grecco, S., Blanc, H. & Vignuzzi, M. (2014). Phy-logenetic and Genome-Wide Deep-Sequencing Anal-yses of Canine Parvovirus Reveal Co-Infection with Field Variants and Emergence of a Recent Recombi-nant Strain. PloS One, 9(11), e111779. DOI: 10.1371/ journal.pone.0111779.

Radzykhovsʹkyy, M. L., & Zayika, S. S. (2017). Patomor-folohichna kharakterystyka parvovirusnoho enterytu v sobak [Pathomorphological characteristics of par-vovirus enteritis in dogs]. Naukovyy visnyk LNUVM ta BT im. S.Z. Gzhytsʹkoho, 19(82), 45–49. DOI: 10.15421/nvlvet8210 (in Ukrainian).

Skrypka, M. V., & Kolych, N. B. (2011). Navchalʹno-metodychnyy posibnyk z patolohichnoyi anatomiyi dlya laboratornykh zanyatʹ [Educational and methodical manual on pathological anatomy for laboratory classes.]. Poltava (in Ukrainian).

Steinel, A., Parrish, C. R., Bloom, M. E. & Truyen, U. (2001). Parvovirus Infections in Wild Carnivores. Journal of Wildlife Diseases, 37(3), 594–607. DOI: 10.7589/0090-3558-37.3.594.

Urbanovych, P. P., Pototsʹkyy, M. K., Hevkan, I. I., & Zon, H. A. (2008). Patolohichna anatomiya tvaryn [Pathological anatomy of animals] Vetinform. Kyiv (in Ukrainian).

Wang, J., Cheng, S., & Cheng, Y. (2012). Evidence for natural recombination between mink enteritis virus and canine parvovirus. Journal of Virology, 9, 252–569. DOI: 10.1186/1743-422X-9-252.

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Published
2022-07-16
How to Cite
Radzykhovskyi, M., Gutyj, B., Dyshkant, O., Sokulsky, I., & Antoniuk, A. (2022). Pathomorphological changes of the stomach and intestinum crassum in the intestinal form of parvoviral enteritis in dogs. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 24(106), 81-86. https://doi.org/10.32718/nvlvet10613