Results of monitoring studies of caecal samples with animal contents for antimicrobial resistance in 2021


  • O. M. Chechet State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, Kyiv, Ukraine https://orcid.org/0000-0001-5099-5577
  • O. S. Haidei State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, Kyiv, Ukraine https://orcid.org/0000-0003-4503-4047
  • V. O. Andriiashchuk State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, Kyiv, Ukraine https://orcid.org/0000-0002-0983-9297
  • O. I. Horbatiuk State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, Kyiv, Ukraine
  • V. L. Kovalenko State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, Kyiv, Ukraine https://orcid.org/0000-0002-9244-6653
  • I. V. Musiiets State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, Kyiv, Ukraine https://orcid.org/0000-0002-2456-560X
  • D. O. Ordynska State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, Kyiv, Ukraine https://orcid.org/0000-0003-3481-3248
  • V. V. Skliar State Scientific and Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise, Kyiv, Ukraine
  • B. V. Gutyj Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Lviv, Ukraine https://orcid.org/0000-0002-5971-8776
  • O. V. Krushelnytska Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Lviv, Ukraine https://orcid.org/0000-0002-4401-8478
Keywords: antibiotic resistance, Escherichia coli, Salmonella spp., Enterococcus spp., Campylobacter spp., state monitoring, antibacterial drugs

Abstract

The article presents the results of studies of caecum samples (cecal appendages) with contents from cattle, pigs, and poultry following the State Monitoring Plan for Antimicrobial Resistance in Veterinary Medicine for 2021, isolated and identified isolates of zoonoses and commensal microorganisms, namely: Escherichia coli, Salmonella spp., Enterococcus faecium, Enterococcus faecalis, Campylobacter spp. The disk diffusion method conducted a study on determining antimicrobial sensitivity to antibacterial drugs. The results of studies on identifying acquired resistance mechanisms to antibacterial drugs are also presented. Interpretation of growth retardation zones was carried out following EUCAST requirements. As a result of the obtained data, 448 isolates were isolated and identified among 2120 samples submitted for research: E coli accounted for 37.7 %, Salmonella spp. – 4.24 %, Enterococcus faecium – 12.7 %, Enterococcus faecalis – 37.9 %, Campylobacter spp. – 7.4 % of all isolated isolates. When determining the sensitivity to antibacterial drugs, eight sensitive isolates were found. 237 isolates were monoresistant (sensitive to 1–2 ABP), and 203 were polyresistant (sensitive to 3 or more ABP). As a result of the research, production (ESBL) was detected and confirmed in ten Escherichia coli strains. Three vancomycin-resistant strains of Enterococcus faecium, and Enterococcus faecalis were identified. Study the prevalence of Escherichia coli, Salmonella spp., Enterococcus faecium, Enterococcus faecalis, Campylobacter spp. circulating in Ukraine. will promote a standardized approach to data collection, analysis, and sharing on a global scale and will ensure the fulfillment of the National Action Plan for Combating Antibiotic Resistance to Antimicrobial Drugs tasks. Ukraine has developed a National Action Plan to combat antimicrobial resistance. In the countries of the European Union, there is constant monitoring for Escherichia coli, Salmonella spp., Enterococcus faecium, Enterococcus faecalis, Campylobacter spp. The prospect of further research is to continue monitoring antimicrobial resistance against zoonoses and commensal bacteria, namely: isolates of Escherichia coli, Salmonella spp., Enterococcus faecium, Enterococcus faecalis, Campylobacter spp., as they play an important epidemiological role among infectious diseases common to humans and animals, determining their sensitivity and identifying acquired mechanisms of resistance to antibacterial drugs.

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Published
2022-07-16
How to Cite
Chechet, O. M., Haidei, O., Andriiashchuk, V., Horbatiuk, O., Kovalenko, V., Musiiets, I., Ordynska, D., Skliar, V., Gutyj, B., & Krushelnytska, O. (2022). Results of monitoring studies of caecal samples with animal contents for antimicrobial resistance in 2021. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 24(106), 128-135. https://doi.org/10.32718/nvlvet10620

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