Antioxidant status of fish with inflammation of the swim bladder in yearling carp


  • O. Frishtak Institute of Fisheries NAAS, Kyiv, Ukraine https://orcid.org/0000-0002-9144-8534
  • M. Koryliak Transcarpathian Research Station of the IRG of the National Academy of Sciences, Mukacheve, Ukraine
  • O. Dobrianska Transcarpathian Research Station of the IRG of the National Academy of Sciences, Mukacheve, Ukraine https://orcid.org/0000-0001-7238-5059
Keywords: carp, swim bladder inflammation (SBA), hepatopancreas, skeletal muscles, antioxidants, lipid peroxidation products

Abstract

The literature data on the etiology and pathogenesis of swim bladder inflammation (SBI) in various fish species have been highlighted in the paper. The main goal of this work was to analyze the impact of the disease on the physiological status of the body of the yearling carp, the state of the antioxidant defense system, and the content of lipid peroxidation products (LPP). The yearling Liubyn scaly carps, with an average weight of 40 g, which were grown in the ponds of the Rivne fish and reclamation station, were selected for experimental research. During control catches and ichthyopathological examination of forty fish, inflammation of the swim bladder was detected with an intensity of 35 %. During the pathological autopsy of this yearling carp, thickening of the walls of the swim bladder with purulent exudate, atrophy of the posterior lobe, and hyperemia of internal organs was found. Data on the content of lipid peroxidation products (LPP) and the state of the antioxidant system (AOS) in the cells of the hepatopancreas and skeletal muscles of this year's carp with SPM have been presented. It was determined that in the hepatopancreas of fish with the swim bladder inflammation, primary and secondary LPP products accumulate, and the activity of AOS enzymes decreases. The increase of diene conjugates in the hepatopancreas of the diseased fish was revealed by 11.8 % compared to the control group, and the content of TBC-active products by 29.8 %. The level of SOD activity is 13.5 % lower in the fish affected by SMP than in the control group. Catalase activity in the hepatopancreas of the diseased fish increases by 11.7 % compared to the control group. There was a 45.5 % decrease in superoxide dismutase activity in the skeletal muscles of fish affected by SBI and a significant increase in the level of catalase (Р < 0.01) compared to the control group. Pathogens that cause diseases of the swim bladder cause also have an inhibitory effect on the activity of enzymes of the antioxidant system, and the content of products of lipid peroxidation increases.

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References

Bachmann, P. A., & Ahne, W. (1973). Isolation and Characterization of Agent causing Swim Bladder Inflammation in Carp. Nature, 244, 235–237. DOI: 10.1038/244235a0.

Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72(1-2), 248–254. DOI: 10.1016/0003-2697(76)90527-3.

Chang, H. K., Chen, Y.-A., Tsai, Y.-L., Wu, Y.-C., Shien, J.-H., Chang, W.-F., Hsuan, S.-L., & Lin C.-C. (2016). Outbreak of swim bladder inflammation caused by Sphaerospora dykovae in Koi (Cyprinus carpio koi) in Taiwan. Pak. Vet. J., 36, 499–502. URL: http://www.pvj.com.pk/abstract/36_4/16-152.htm.

Chen, Y., Li, M., Yuan, L., Xie, Y., Li, B., Xu, W., Meng, F., & Wang, R. (2017). Growth, blood health, antioxidant status and immune response in juvenile yellow catfish Pelteobagrus fulvidraco exposed to α-ethinylestradiol (EE2). Fish Shellfish Immunol, 69, 1–5. DOI: 10.1016/j.fsi.2017.08.003.

Davidov, O. N., & Temnihanov, Yu. D. (2004). Bolezni presnovodnyih ryib, K., TOV “Vetinform” (in Russian).

Dubinina, E. E., Sal'nikova, L. A., & Efimova, L. F. (1983). Aktivnost' i izofermentnyy spektr superoksiddismutazy eritrotsitov i plazmy krovi cheloveka [Activity and isoenzyme spectrum of erythrocyte superoxide dismutase and human blood plasma]. Lab. Delo, 10, 30–33 (in Russian).

Eszterbauer, E., Sipos, D., Forró B., Bartošová, P., & Holzer, A. S. (2013). Molecular characterization of Sphaerospora molnari (Myxozoa), the agent of gill sphaerosporosis in common carp Cyprinus carpio carpio. Dis Aquat Organ, 104, 59–67. DOI: 10.3354/dao02584.

Holzer, A. S., Hartigan, A., Patra, S., Pecková, H., & Eszterbauer, E. (2014). Molecular fingerprinting of the myxozoan community in common carp suffering Swim Bladder Inflammation (SBI) identifies multiple etiological agents. Parasites Vectors, 7, 398. DOI: 10.1186/1756-3305-7-398.

Jara, Z., & Chodyniecki, A. (1999). Ichtiopatologia. Wroclaw., Wydaw. Akademii Rolniczej (in Poland).

Karysheva, A. F. (2002). Spetsialna epizootolohiia. Kyiv, Vyshcha osvita (in Ukrainian).

Kocylowski, B., Antychowicz, J., & Zelazny, J. (1970). Studies on the etiology and pathogenesis of carp swimbladder inflammation. Riv. It. Piscic. Ittiopat., 5, 59.

Korobeynikova, E. N. (1989). Modifikatsiya opredeleniya produktov perekisnogo okisleniya lipidov v reaktsii s tiobarbiturovoy kislotoy [Modification of the determination of lipid peroxidation products in the reaction with thiobarbituric acid]. Lab. Delo, 7, 8–9 (in Russian).

Korolyuk, M. A., Ivanova, L. I., & Mayorova, I. G. (1988). Metod opredeleniya aktivnosti katalazy. Laboratornoe delo. [Method for determining catalase activity]. Laboratornoe delo, 1, 16–19 (in Russian).

Lavryshyn, Y. Y., Varkholyak, I. S., Martyschuk, T. V., Guta Z. А. & Ivankiv L. B. (2016). Biolohichne znachennia systemy antyoksydantnoho zakhystu orhanizmu tvaryn. Naukovyi visnyk LNUVMBT imeni S. Z. Gzhytskoho, 18(2(66), 100–111. DOI: 10.15421/nvlvet6622.

Martinez-Alvarez, R. M., Morales, A. E., & Sanz А. (2005). Antioxidant defenses in fish: biotic and abiotic factors. Rev. Fish Biol. Fish., 15(1), 75–88. DOI: 10.1007/s11160-005-7846-4.

Martyshuk, T. V., Gutyj, B. V., & Khalak, V. I. (2021). System of antioxidant protection of the body of piglets under the action of feed additive “Butaselmevit-plus”. Ukrainian Journal of Veterinary and Agricultural Sciences, 4(2), 38–43. DOI: 10.32718/ujvas4-2.07.

Mikheev, V. N., Pasternak, A. F., & Valtonen, E. (2014). Increased ventilation by fish leads to a higher risk of parasitism. Parasit Vectors, 7, 281. DOI: 10.1186/s13071-015-1005-0.

Nilsson, G. E., & Renshaw, G. M. C. (2004). Hypoxic survival strategies in two fishes : extreme anoxia tolerance in the North European crucian carp and natural hypoxic preconditioning in a coral-reef shark. The J. of Exp. Biol., 207, 3131–3139. DOI: 10.1242/jeb.00979.

Oleksiuk, N. P. (2011). Sezonni zminy peroksydnykh protsesiv i aktyvnosti antyoksydantnoi systemy u ziabrakh prisnovodnykh ryb. Biolohiia tvaryn, 13(1-2), 140–147 (in Ukrainian).

Rohlenová, K., Morand, S., Hyršl, P., Tolárová, S., Flajšhans, M., & Šimková, A. (2011). Are fish immune systems really affected by parasites? An immunoecological study of common carp (Cyprinus carpio). Parasit Vectors, 4, 120. DOI: 10.1186/1756-3305-4-120.

Schulz-Mirbach, T., Metscher, B., & Ladich, F. (2012). Relationship between Swim Bladder Morphology and Hearing Abilities. A Case Study on Asian and African Cichlids. PloS ONE, 7(8), e42292. DOI: 10.1371/journal.pone.0042292.

Sekretariuk, K. V., Danko, M. M., & Stybel, V. V. (2002). Veterynarna sanitariia i hihiiena v rybnytstvi. M., Unyversum Pablyshynh (in Ukrainian).

Stal'naya, I. D. (1977). Metod opredeleniya dienovoy kon'yugatsii nenasyshchenykh vysshikh zhirnikh kislot [Method for determination of diene conjugation of unsaturated higher fatty acids]. Sovremennye metody v biokhimii [Modern methods in biochemistry]. M. Meditsina, 63–64 (in Russian).

Storey, K. B. (1996). Oxidative stress: animal adaptations in nature. Bras. J. Med. Biol. Res., 29(12), 1715–1733. URL: https://pubmed.ncbi.nlm.nih.gov/9222437.

Varkholiak, I. S., Gutyj, B. V., Leskiv, Kh. Ya., Kushnir, V. I., Hariv, I. I., Martyshuk, T. V., & Guta, Z. A. (2021). The effect of bendamine on antioxidant protection of rats' myocardium in doxorubicin intoxication. Colloquium-journal, 7(94), 18–21. DOI: 10.24412/2520-6990-2021-794-18-21.

Vovk, N. I. & Bozhyk, V. Y. (2014). Ikhtiopatolohiia. Pidruchnyk. Kyiv, Ahroosvita (in Ukrainian).

Vyslotska, L. V., Gutyj, B. V., Kozenko, O. V., Khalak, V. I., Chornyj, M. V. Martyshuk, T. V., Krempa, N. Yu., Vozna, O. Ye., & Todoriuk, V. B. (2021). System of antioxidant protection of the body of piglets under the action of feed additive “Sylimevit”. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. Series: Veterinary sciences, 23(104), 10–17. DOI: 10.32718/nvlvet10402.

Zhang, J., Shen, H., Wang, X., Wu, J., & Xue, Y. (2004). Effects of chronic exposure of 2,4-dichlorophenol on the antioxidant system in liver of freshwater fish Carassius auratus. Chemosphere, 55(2), 167–174. DOI: 10.1016/j.chemosphere.2003.10.048.

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Published
2022-07-16
How to Cite
Frishtak, O., Koryliak, M., & Dobrianska, O. (2022). Antioxidant status of fish with inflammation of the swim bladder in yearling carp. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 24(106), 172-176. https://doi.org/10.32718/nvlvet10626