INFLUENCE ALLOGENEIC MESENCHYMAL STEM CELLS IN PERITONEAL MACROPHAGES OKSYHENZALEZHNYY METABOLISM MICE S57BL / 6


  • L. Kladnytska NULESU
  • A. Mazurkiewicz NULESU
  • S. Velichko NULESU
  • V. Kovpak NULESU
  • A. Dzhus NULESU
  • D. Shelest NULESU
Keywords: peritoneal macrophages, allogeneic mesenchymal stem cells, oksyhenzalezhnyy metabolism, spontaneous activity, stimulated activity, mouse, Lewis carcinoma

Abstract

The study was conducted on male mice C57BL/6 weighing 20-22 g aged 2-3 months. Receiving allogeneic MSCs cultivation of primary material that was isolated from the bone marrow of mice C57BL/6. Cultivation of cells was carried out in DMEM medium with addition of 20% fetal bovis serum (FBS) and 1% antibiotic-antimycotics (Sigma, USA) at 37 °C, 100% humidity and 5% CO2.

It was inoculated intramuscularly cell suspension metastatic Lewis lung carcinoma (LLC) in a concentration 1×106/0.1 ml Hanks to mice C57BL/6. On the 8th day after tumor cell inoculation it was administered intravenously allogeneic MSCs in a concentration 1,25×104  to 4th  group of animals. After that was formed following groups of animals: 1st included intact animals (control), 2nd – included animals which was administered only allogeneic mesenchymal stem cells (MSC), 3rd  – included animals which was administered suspension metastatic Lewis lung carcinoma (LLC ),  4th  group  – which was administered suspension metastatic Lewis lung carcinoma and allogeneic MSCs (LLC + MSC).

Spontaneous and stimulated oxidative metabolism of peritoneal macrophages were established  in NBT-test.

It was established that administration  allogeneic MSCs   influence on  oxidative metabolism of peritoneal macrophages in mice C57BL/6. The use of allogeneic MSCs provides a probable decrease metabolic activity of peritoneal macrophages in miceC57BL/6 with stimulation  index -12%. It was indicate  that  cells lose of functional reserve. In mice with  Lewis lung carcinoma ( 3rd  group of animals)  metabolic activity of peritoneal macrophages in mice C57BL/6   was decreased  with stimulation  index  -30% like  indicating a losek of cells. functional reserve.

Allogeneic MSCs application in mice C57BL/6 perescheplenoyu Lewis lung carcinoma is lead to a slight increase metabolic activity of peritoneal macrophages   with stimulation  index  8%, which indicates the presence of cells functional reserve.


Downloads

Download data is not yet available.

Author Biographies

L. Kladnytska, NULESU
k.vet.n., Associate Professor
A. Mazurkiewicz, NULESU
d.vet.n., Professor
S. Velichko, NULESU
PhD
V. Kovpak, NULESU
k.vet.n., senior teacher
A. Dzhus, NULESU
graduate student
D. Shelest, NULESU
PhD student

References

Keating, A. Mesenchymal stromal cells. Curr Opin Hematol. 2006; 13: 419–425.

Geissmann, F., Manz, M. G., Jung, S., Sieweke, M. H., Merad, M., Ley K. Development of monocytes, macrophages, and dendritic cells. Science. 2010; 327: 656–661.

Nauta, A. J., Fibbe, W. E. Immunomodulatory properties of mesenchymal stromal cells. Blood. 2007;110:3499–3506.

Uccelli, A., Moretta, L., Pistoia, V. Mesenchymal stem cells in health and disease. Nat Rev Immunol. 2008;8:726–736

Jiang, X. X., Zhang, Y., Liu, B., Zhang, S. X., Wu, Y., et al. Human mesenchymal stem cells inhibit differentiation and function of monocyte-derived dendritic cells. Blood. 2005; 105: 4120–4126.

Julian Maggini Mouse Bone Marrow-Derived Mesenchymal Stromal Cells Turn Activated Macrophages into a Regulatory-Like Profile // Julian Maggini, Gerardo Mirkin, Ianina Bognanni, Josefina Holmberg, Isabel M. Piazzón, Irene Nepomnaschy, Héctor Costa, Cristian Cañones, Silvina Raiden,1 Mónica Vermeulen,1andJorge R. Geffner PLoS One. 2010; 5(2): e9252.Published online 2010 Feb 16. doi: 10.1371/journal.pone.0009252

Li, Y. P., Paczesny, S., Lauret, E., Poirault, S., Bordigoni, P. Human mesenchymal stem cells license adult CD34+ hemopoietic progenitor cells to differentiate into regulatory dendritic cells through activation of the Notch pathway. J Immunol. 2008;180: 1598–1608.

Spaggiari, G. M., Capobianco A., Becchetti, S., Mingari, M. C., Moretta, L. Mesenchymal stem cells-natural killer cell interactions: evidence that activated NK cells are capable of killing MSCs, whereas MSCs can inhibit IL-2-induced NK-cell proliferation. Blood. 2006; 107: 1484–1490.

Spaggiari, G. M., Capobianco, A., Abdelrazik, H., Becchetti, F., Mingari, M. C., et al. Mesenchymal stem cells inhibit natural-killer-cell proliferation, cytotoxicity, and cytokine production: role of indoleamine 2,3-dioxygenase and prostaglandin E2. Blood. 2008; 111:1327–1333.

Krampera, M., Glennie, S., Dyson, J., Scott, D., Laylor, R. Bone marrow mesenchymal stem cells inhibit the response of naïve and memory antigen-specific T cells to their cognate peptide. Blood. 2003; 101: 3722–3729.

Keating, A. How do mesenchymal stromal cells suppress T cells? Cell Stem Cell. 2008; 2: 106–108 .

Rasmusson, I., Ringdén, O., Sundberg, B., Le Blanc, K. Mesenchymal stem cells inhibit the formation of cytotoxic T lymphocytes, but not activated cytotoxic T lymphocytes or natural killer cells. Transplantation. 2003; 76: 1208–1213.

Corcione, A., Benvenuto, F., Ferretti, E., Giunti, D., Cappiello, V., et al. Human mesenchymal stem cells modulate B-cell functions. Blood. 2006; 107: 367–372.

Coligan, J. E., Kruibeek, A. M., Margulies, D. H. Morphological and biochemical assays of apoptosis. 1994. In: Current Protocols in Immunology. Vol 3, New York: Wiley.

Puddu, P., Fantuzzi, L., Borghi, P., Varano, B., Rainaldi, G., et al. IL-12 induces IFN-gamma expression and secretion in mouse peritoneal macrophages. J Immunol. 1997;159: 3490–3497.

Mantovani, A., Sica, A. Macrophages, innate immunity and cancer: balance, tolerance, and diversity // Curr. Opin. Immunol. – 2010. – 22, No 2. – P. 231–237.

Munder, M., Mallo, M., Eichmann, K., Modolell, M. Murine macrophages secrete interferon gamma upon combined stimulation with interleukin (IL)-12 and IL-18: A novel pathway of autocrine macrophage activation. J Exp Med. 1998;187:2103–2108.

Schleicher, U., Hesse, A., Bogdan, C. Minute numbers of contaminant CD8+ T cells or CD11b+CD11c+ NK cells are the source of IFN-γ in IL-12/IL-18 stimulated mouse macrophage populations. Blood. 2005; 105: 1319–1328.

Mosser, D. M., Edwards, J. P. Exploring the full spectrum of macrophage activation. Nat Rev Immunol. 2008; 8: 958–969.

Erwig, L. P., Henson, P. M. Clearance of apoptotic cells by phagocytes. Cell Death Differ. 2008;15: 243–250.

Moncayo, A., Ortiz Yanine, M. I. An update on Chagas disease (human American tripanosomiasis). Ann Trop Med Parasitol. 2006; 100: 663–677.

Stempin, C., Giordanengo, L., Gea, S., Cerbán, F. Alternative activation and increase of Trypasosoma cruzi survival in murine macrophages stimulated by cruzipain, a parasite antigen. J Leuk Biol. 2002; 72: 727–734.

Németh, K., Leelahavanichkul, A., Yuen, P. S., Mayer, B., Parmelee, A., et al. Bone marrow stromal cells attenuate sepsis via prostaglandin E2-dependent reprogramming of host macrophages to increase their IL-10 production.Nat Med. 2009; 15: 42–49.

Mazurkevich, A. Y., Kladnytska, L. V., Kovpak, V. V. Features conditions selection and cultivation mouse bone marrov adhesive fraction mononuclear cells. Bulletin of Taras Shevchenko National University of Kyiv – Vestnik; 2013, Vol. 64 Issue 2, pp. 41–43.

Park, B.H., Fikrig, S.M., Smithwich, E.M. Infection and nitrobluetetrazolium reduction by neutrophils; a diagnostic aid. Lancet. 1968. V. 11 (2). P. 532–534.

Zhang, X. The isolation and characterization of murine macrophages Zhang X. Goncalves R. Mosser D. //Curr.Protoc.Immunol. – 2008. – Chapter 14 unit 14.1 – P.1411–1414.

Abstract views: 72
PDF Downloads: 56
Published
2015-04-01
How to Cite
Kladnytska, L., Mazurkiewicz, A., Velichko, S., Kovpak, V., Dzhus, A., & Shelest, D. (2015). INFLUENCE ALLOGENEIC MESENCHYMAL STEM CELLS IN PERITONEAL MACROPHAGES OKSYHENZALEZHNYY METABOLISM MICE S57BL / 6. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 17(3), 49-56. Retrieved from https://nvlvet.com.ua/index.php/journal/article/view/517
Section
Physiological-biochemical and biotechnological ways of animal productivity increasing