THE EFFECT OF BSA AND REDUCED GLUTATHIONE AS PART OF THE ENVIRONMENT FOR FREEZING THE RAM SPERM ON THE FROZEN-THAWED SPERM QUALITATIVE CHARACTERISTICS
Abstract
The research findings on the determination of the optimal concentration of reduced glutathione and bovine serum albumin in the lactose-yolk-tris-citrate-glycerine diluent with ram-sires sperm cryopreservation are revealed. It is established that theaddition of 5 µМ of reduced glutathione and 15 mg/ml of bovine serum albumin into the lactose-yolk-tris-citrate-glycerine diluent contributed to the increase in frozen-thawed sperm activity by 11,0 %, its resistance to cryopreservation by 6,0 %, intact acrosome by 9,5 %, sperm surviving rate by 23,9 %, absolute survival indicator by 21,8 %, sperm kinetic indicators: VCL – by 14,3 %, VAP – by 12,5 %, VSL – by 15,4 %, LIN – by 0,4 %, STR – by 2,2%, which indicates a positive effect of the added bioactive substances into the diluent on the qualitative characteristics of frozen-thawed sperm. At the same time observed the reduction in the number of TBA-active products in seminal plasma after equilibration by 22,2 % (p<0,01), after sperm thawing – 27,4 % (p<0,01) respectively in comparison with the control.
Assessment of the reproductive indicators of ewes showed that the proposed improved diluent increases the biological value of sperm and their fertilizing capacity. In particular, the fertility of ewes inseminated with frozen-thawed sperm frozen in the improved diluent was higher by 12,0 % in comparison with the animals, inseminated with the frozen sperm using the control diluent.
Downloads
References
Salamon, S. Frozen storage of ram semen I. Processing, freezing, thawing and fertility after cervical insemination / S. Salamon, W. M. C. Maxwell // Animal Reproduction Science. - 1995. - Vol. 37. - P. 185-249.
Grigorjan, S. Dejstvie antioksidantov na spermu barana pri glubokom ee zamorazhivanii / S. Grigorjan, V. Nazarjan // Zhivotnovodstvo. 1981. № 9. S. 49-50. (in Russian).
Budai, C. The protective effect of antioxidants on liquid and frozen stored ram semen - Review / C. Budai, I. Egerszegi, J. Olah, A. Javor, A. Kovacs // Animal Science and Biotechnologies. - 2014. - Vol. 46 - P. 46-52.
Solouma, G. M. The influence of adding glutathione on semen characteristics of sohagi rams / G. M. Solouma // Egyptian Journal of Sheep & Goat Sciences. - 2013. - Vol. 8 (2). - P. 61-68.
Uysal, O. Effects of oxidized glutathione, bovine serum albumin, cysteine and lycopene on the quality of frozen-thawed ram semen / O. Uysal, M. N. Bucak // ACTA VET. BRNO. - 2007. - № 76. - P. 383-390.
Tvrdá, E. Bovine serum albumin as a potential protein supplement for in viro cultivation of spermatozoa / E. Tvrdá, Z. Kňažická, P. Massányi, R. Stawarz, G. Formicki, N. Lukáč // MENDELNET. - 2010. - P. 964-969.
Ali, A. Studies of the effect of semi-defined diluents on the cryo-tolerance of spermatozoa and in vitro production embryos / A. Ali, B. Talib // ANNO ACCADEMICO. - 2009-2010. - 138 р.
Ashrafi, I. Antioxidant effects of bovine serum albumin on kinetics, microscopic and oxidative characters of cryopreserved bull spermatozoa / I. Ashrafi, H. Kohram, H. Tayefi-Nasrabadi // Spanish Journal of Agricultural Research. - 2013. - № 11 (3). - P. 695-701.
Sokolovskaja, I. O Znachenii akrosomy v ocenke semeni samcov / I. I. Sokolovskaja, R. N. Ojvadis, A. Abilov // Zhivotnovodstvo. 1981. - № 9. - S. 46–47. (in Russian).
Abstract views: 63 PDF Downloads: 34
This work is licensed under a Creative Commons Attribution 4.0 International License.