THE EFFECT OF BSA AND REDUCED GLUTATHIONE AS PART OF THE ENVIRONMENT FOR FREEZING THE RAM SPERM ON THE FROZEN-THAWED SPERM QUALITATIVE CHARACTERISTICS


  • C. Grymak Institute of animal biology NAAS, Lviv
Keywords: ram-sires, ewes, cryopreservation, frozen-thawed sperm, sperm kinetic parameters, reduced glutathione, bovine serum albumin, TBA-active products, the fertilizing ability, lambing, lambs, body weight

Abstract

The research findings on the determination of the optimal concentration of reduced glutathione and bovine serum albumin in the lactose-yolk-tris-citrate-glycerine diluent  with ram-sires sperm cryopreservation are revealed. It is established that theaddition of 5 µМ of reduced glutathione and 15 mg/ml of bovine serum albumin into the lactose-yolk-tris-citrate-glycerine diluent contributed to the increase in frozen-thawed  sperm activity by 11,0 %, its resistance to cryopreservation by 6,0 %, intact acrosome by 9,5 %, sperm surviving rate by 23,9 %, absolute survival indicator by 21,8 %, sperm kinetic indicators: VCL  by 14,3 %, VAP  by 12,5 %, VSL  by 15,4 %, LIN  by 0,4 %,  STR  by 2,2%,  which indicates a positive effect of the added bioactive substances into the diluent on the qualitative characteristics of frozen-thawed sperm.  At the same time observed the reduction in the number of TBA-active products in seminal plasma after equilibration by  22,2 % (p<0,01), after sperm thawing – 27,4 % (p<0,01) respectively in comparison with the control.

Assessment of the reproductive indicators of ewes showed that the proposed improved diluent increases the biological value of sperm and their fertilizing capacity. In particular, the fertility of ewes inseminated with frozen-thawed sperm frozen in the improved diluent was higher by 12,0 % in comparison with the animals, inseminated with the frozen sperm using the control diluent.

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Author Biography

C. Grymak, Institute of animal biology NAAS, Lviv
Candidate of Agricultural Sciences

References

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Published
2015-04-10
How to Cite
Grymak, C. (2015). THE EFFECT OF BSA AND REDUCED GLUTATHIONE AS PART OF THE ENVIRONMENT FOR FREEZING THE RAM SPERM ON THE FROZEN-THAWED SPERM QUALITATIVE CHARACTERISTICS. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 17(3), 154-161. Retrieved from https://nvlvet.com.ua/index.php/journal/article/view/538
Section
Producing of feedstuffs, nourishment, selection and animal breeding