• B.O. Chernushkin Lviv national university of veterinary medicine and biotechnologies
  • L.G. Slivinska Lviv national university of veterinary medicine and biotechnologies
  • V.V. Vlizlo Institute of Animal Biology NAAS
Keywords: sheep, hepatodystrophy, oxidative stress, TBA-active products of lipid hydroperoxides, diene conjugates, hepatocytes


The article deals with the results of studies on the causes and clinical manifestations of hepatodystrophy in ewes, indicators status of lipid peroxidation – TBA-active products, lipid hydroperoxides, diene conjugates.

In order to establish the causes of hepatodystrophy in sheep, we have analyzed the feed rations. Sheep investigated 24 local breeds aged 3 – 4 years, weighing 45 – 56 kg. Conducted clinical study animals and blood were collected for laboratory analysis. Serum sheep determined the content of malondialdehyde (MDA-active products) using thiobarbituric acid (Stalnaja I.D., Haryshvyly T.G, 1977) and lipid hydroperoxides using ammonium thiocyanate (Romanova LA, Stalnaja I.D., 1977). In blood examined diene conjugates (Stalnaja I.D., 1977).

High performance is ensured animals by creating appropriate technical conditions and feeding. Violation of maintenance processes and non-biological nutrition of the cause performance degradation, the spread of disease and culling of animals. Development hepatodystrophy animals observed in animals fed low-quality forage and depraved that contain a number of toxic products of oxidation of fats – aldehydes, ketones, low molecular weight acids, hydroxy acids, which have toxic properties and also due to an unbalanced diet for the main nutrients and biologically active substances

The main causes of primary hepatodystrophy in sheep is imbalanced nutrition and lack of essential nutrients in the diet. In analyzing the diet of established lack of dry matter, raw protein, digestible protein, raw fiber, sugar and starch. Value sugar and starch with digestible protein accounted for 1,78: 1, and sugar-protein ratio – 0,77. Rations were scarce for vitamins D and E, microelements – Sulphur, copper, zinc, cobalt and iodine.

In ewes suffering from hepatodystrophy was set the development of oxidative stress – increase in blood activity of TBA-active products, lipid hydroperoxides, diene conjugates. In particular, concentration of diene conjugates was increased by twice the number of hydroperoxides lipids was increased by three times and the activity of TBA-active products was increased by five times compared with clinically healthy animals. Histological examination of liver biopsies of ewes was set that the develop of protein granular and fatty degeneration of hepatocytes.


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How to Cite
Chernushkin, B., Slivinska, L., & Vlizlo, V. (2016). INDICATORS OF LIPIDS PEROXIDATION IN THE BLOOD OF EWES WITH HEPATODYSTROPHY. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 18(2(66), 202-205.