THE NATURAL RESISTANCE’S CONDITION OF SOWS UNDER THE INFLUENCE OF THE PREPARATION «IMUNOLAC»


  • A. Kokarev Dnipropetrovsk state agrarian–economic university, Voroshilov Str., 25, Dnepr, 49600
  • D. Masiuk Dnipropetrovsk state agrarian–economic university, Voroshilov Str., 25, Dnepr, 49600
Keywords: natural resistance, sows, bactericidal activity of blood serum, lysozyme serum activity, circulating immune complexes, red blood cells, white blood cells, hemoglobin, hematocrit, gestation

Abstract

The article presents data on the state of morphological, biochemical and immunological parameters of natural resistance of sows during the late period of gestation and influence of the preparation enzymatic hydrolyzate of the cell wall Lactobacillus Delbrueckii «Imunolak» on these parameters. Aim of this work was to investigate the state of the natural resistance of sows in the second half of gestation under the action of immunotropic preparation «Imunolak». This work was done in the SIC of biosafety and environmental control of agriculture resources of Dnipropetrovsk state agrarian–economic university and in the PrAT «Agro–Soyuz» («Agro–Soyuz», JSC) Synel’nykovskoe district of Dnepropetrovsk region. For the experiment were formed research and control groups of sows hybrids of white and Landrace breeds on the pair’s analogues principle. Each group consisted of 10 animals. Sows of research group were injected preparation «Imunolak» at 60, 75 and 90 days of gestation (TU 21.2–00493675–006:2013) at a dose of 0.05 mg of active ingredient per 1 kg of body weight, and the animals of the control group were injected 0.9% solution of NaCl. Blood samples were taken at 60, 75, 90 and 105 day of gestation. Morphological blood parameters were determined using an automatic hematology analyzer «PCE–90 VET» (USA). Serum bactericidal activity was determined by fotonefelometric method by assessment of oppression the growth of the test culture of E. coli in meat infusion broth by the method of Michel and Treffers (1956) in the modification of I. Hrabustovskiy and Y. Markova (1974). Lysozyme activity in the sow’s blood serum and colostrums were determined by I. Hrabustovskiy with co–authors (1974). Numbers of circulating immune complexes in serum were determined by PEG–test (Y.A. Grinevich, 1988). The obtained results indicate that late period of gestation in sows characterized by a decrease in hemoglobin levels and in the number of circulating immune complexes on the 75th and 105th days of gestation by 17.5% (р ≤ 0.01) and 21.6% (p ≤ 0.05) and 12.2% (р ≤ 0.05) and 23.0% (р ≤ 0.05) respectively. Also the late period of gestation characterized by a decrease on the 90th days the number of leukocytes by 14.5% and a decrease during second half of gestation period the hematocrit index by 19.72% (р ≤ 0.01) concerning the 60th day of gestating. The use of the preparation «Imunolak» contributes to increase in sow’s blood the hemoglobin levels, the number of red blood cells, white blood cells and hematocrit index on the average by 13.8%, 10.9%, 27.6% and 10.2% respectively (р ≤ 0,05 – р ≤ 0,01) and to increase the bactericidal and lysozyme serum activity by 23.0% (r ≤ 0,05) and 22.5% (r ≤ 0,05) respectively.

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Published
2016-10-07
How to Cite
Kokarev, A., & Masiuk, D. (2016). THE NATURAL RESISTANCE’S CONDITION OF SOWS UNDER THE INFLUENCE OF THE PREPARATION «IMUNOLAC». Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 18(4(72), 32-36. Retrieved from https://nvlvet.com.ua/index.php/journal/article/view/980